BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Identification of the Interaction between Rat Translationally Controlled Tumor Protein/IgE-dependent Histamine Releasing Factor and Myosin Light Chain

  • Kim, Min-Jeong (College of Pharmacy, Center for Cell Signaling Research, Division of Molecular Life Sciences, Ewha Womans University) ;
  • Jung, Jae-Hoon (Center for Cell Signaling Research, Division of Molecular Life Sciences, Medical School, Ewha Womans University) ;
  • Choi, Eung-Chil (College of Pharmacy, Seoul National University) ;
  • Park, Hae-Young (Medical School, Ewha Womans University) ;
  • Lee, Kyung-Lim (College of Pharmacy, Center for Cell Signaling Research, Division of Molecular Life Sciences, Ewha Womans University)
  • Received : 2001.07.11
  • Accepted : 2001.08.17
  • Published : 2001.11.30
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Abstract

The translationally controlled tumor protein (TCTP), also known as the IgE-dependent histamine releasing factor (HRF), was used in the yeast two-hybrid system to screen the interacting molecules. We obtained the N-terminus truncated rat fast myosin alkai light chain from the rat skeletal muscle cDNA library in the screening. Since either TCTP/HRF or the myosin light chain is known to be associated with histamine secretion from RBL-2H3 cells, we investigated the possible interaction between rat TCTP/HRF and nonmuscle myosin light chain in these cells. We used affinity chromatography and coimmunoprecipitation. Our data suggests that HRF and the myosin light chain interact, which may play an important role in histamine release in RBL-2H3 cells.

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