Journal of the Korean Chemical Society (대한화학회지)

Korean Chemical Society (대한화학회)
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Quantitative Analysis of Microcystins, Cyanobacterial Toxins in Soyang Lake

소양호에서 남조류 독소, 마이크로시틴의 정량 분석

  • Lee, Jeong Ae (Department of chemistry, Kangwon National University) ;
  • Lee, So Yeong (Department of chemistry, Kangwon National University) ;
  • Pyo, Dong Jin (Department of chemistry, Kangwon National University)
  • 이정애 (강원대학교 자연과학대학 화학과) ;
  • 이소영 (강원대학교 자연과학대학 화학과) ;
  • 표동진 (강원대학교 자연과학대학 화학과)
  • Published : 2002.12.20
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Abstract

It is very difficult to analyze the microcystins, cyanobacterial toxins quantitatively since it exists in a trace level in lakes. In this paper, two different analytical methods were tried to analyze the microcystins, cyanobacterial toxins quantitatively in water samples collected in Soyang lake. The first method was solid phase extraction method fol-lowed by High Performance Liquid Chromatography(HPLC), and the second method was Enzyme-Linked Immu-nosorbent Assay(ELISA) using the monoclonal antibody of microcystin.

남조류 독소인 마이크로시틴은 호수에서 매우 미량 존재하기 때문에 이를 정확히 정량 분석하기가 쉽지 않다. 본 연구에서는 소양호에서 채취한 물시료 중에 포함된 남조류 독소, 마이크로시틴을 정량 분석하기 위하여 두 가지 서로 다른 정량 분석법을 시도하였으며 이 둘의 정량 결과를 비교${\cdot}$분석 하였다. 첫째는 고체상 추출과 HPLC(High Performance Liquid Chromatography)를 이용한 분석법을 이용하였으며 둘째는 마이크로시틴의 단일클론항체를 이용한 효소면역흡착분석법(Enzyme-Linked Immunosorbent Assay,ELISA)을 이용하였다.

Keywords

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