DOI QR코드

DOI QR Code

H-약침(藥鍼)의 급성(急性).아급성(亞急性) 독성실험(毒性實驗) 및 항암효과(抗癌效果)에 관(關)한 실험적(實驗的) 연구(硏究)

The Study on Acute.Subacute Toxicity and Anti-cancer Effect of H Herbal-acupuncture

  • 김태희 (상지대학교 한의과대학 침구학교실) ;
  • 권기록 (상지대학교 한의과대학 침구학교실) ;
  • 이선구 (상지대학교 한의과대학 병리학교실)
  • Kim, Tae-Hui (Dept. of Acupuncture & Moxibustion, Oriental Medical college Sangji University) ;
  • Kwon, Ki-Rok (Dept. of Acupuncture & Moxibustion, Oriental Medical college Sangji University) ;
  • Lee, Seon-Goo (Department of Pathology, Oriental Medical college Sangji University)
  • 발행 : 2002.12.01

초록

Objectives : The purpose of this study is to investigate Acute and Subacute Toxicity, and Anti-cancer Effect of H Herbal-acupuncture on mice and rats. Methods : Balb/c mice were injected intraperitoneally with H Herbal-acupuncture for $LD_{50}$ and acute toxicity test. Sprague-Dawley rats were experimented in the same way for subacute toxicity test. H Herbal-acupuncture was injected into abdomen of mice having S-180 cancer cell line. Result : 1. During the test, $LD_{50}$ could not be counted since there was no expired subjects. 2. In an acute toxicity test, the loss of motility and reflex action was observed, but weight increased in the treatment group, compared with those in the normal group (P<0.05). 3. In an acute toxicity test of serum biochemical values of mice, glucose increased in the treatment group II while total cholesterol was increased in the all treatment groups (P<0.05). 4. In a subacute toxicity test, a little loss of motility and reflex action was observed in the treatment group. Weight of mice in the treatment group decreased on the 28th day. 5. In a subacute toxicity test, liver weight was decreased but lung weight of mice increased in the all treatment groups (P<0.05). 6. As a result of measuring Complete Blood Count test (CBC) of rat, HCT was decreased in treatments even though it was not significant, compared with the normal group (P<0.05). 7. In a serum biochemical value test of subacute toxicity, total protein and albumin decreased in the all treatment groups. Creatinine, glucose, GOT increased in the treatment group I compared with the control group. Alkaline phos-phatase decreased in treatment II group, compared with the control group (P<0.05). 8. Median survival time that was measured in the rats treated with sarcoma-180 cancer cell Median decreased in the treatment group, compared with the control group (P<0.05). 9. Natural killer cell activity showed significant reduction at 100:1 and 10:1 E/T ratio while it increased at 50:1 E/T ratio. It is inferred that there was an error in the experiment (P<0.05). 10. In an interleukin-2 productivity test, even though it decreased in lung cancer, and increased in abdomen cancer, but it was only a small difference (P<0.005). 11. After injecting B16F10 cell into a capillary vessel of C57BL/6 mice and generating metastasized lung cancer, the lung was examined with the naked eye. It was not possible to see metastasized cancer in the all groups on the seventh day but the cancer was viewed on the fourteenth day. The number and volume of metastasized cancer in the treatment group enlarged in the treatment group, compared with the control group. Conclusion : According to the results, H herbal-acupuncture took no effects in cancer.

키워드

참고문헌

  1. 대한면역학회지 v.8 no.1 암면역학과 면역요법 김진복
  2. 병리학 I 대한병리학회
  3. 약침요법 시술 지침서 대한약침학회
  4. 대한약침학회지 v.4 no.3 호도약침의 급성${\cdot}$아급성 독성에 관한 실험적 연구 강계성(외) https://doi.org/10.3831/KPI.2001.4.3.085
  5. 대한약침학회지 v.5 no.1 호도약침의 Sarcoma-180 항암효과에 관한 실험적 연구 이선구(외) https://doi.org/10.3831/KPI.2002.5.1.043
  6. 대한약침학회지 v.5 no.1 홍화자약침의 급성${\cdot}$아급성 독성 및 Sarcoma-180 항암효과에 관한 실험적 연구 안창석(외) https://doi.org/10.3831/KPI.2002.5.1.007
  7. 대한약침학회지 v.5 no.1 파두약침의 급성${\cdot}$아급성 독성 및 Sarcoma-180 항암효과에 간한 실험적 연구 유창길(외) https://doi.org/10.3831/KPI.2002.5.1.027
  8. 대한약침학회지 v.5 no.1 행인약침의 급성${\cdot}$아급성 독성 및 Sarcoma-180 항암효과에 관한 실험적 연구 김옥(외) https://doi.org/10.3831/KPI.2002.5.1.061
  9. 癌瘤防治硏究 賈곤
  10. 대통령 각하 ! 암을 정복했습니다 한동규
  11. 의약품 독성시험기준 제 98-116호 식품의약품안전본부
  12. Cancer chemotherapy Reports Protocol for screening chemical Agents and Natural products against Animal Tumors and other Biological system Geran, R.I.;Greenberg, N.H.;Macdinald, M.M.;Schumacher, A.M.;Abbot, B.J.
  13. 종양학 서울대학교 의과대학
  14. Current Medical Diagnosis and Treatment 전국의과대학교수(譯)
  15. 東醫腫瘍學 崔卑勳
  16. 황제내경 소문영추해석 楊維傑(編)
  17. 대한한방종양학회지 v.9 no.2 암종의 병인병리에 관한 문헌적 고찰 田炳旭(外)
  18. 大韓韓醫學會誌 v.9 no.2 人蔘水鍼 前處置가 發癌豫防에 미치는 影響 金泰潤
  19. 大韓韓醫學會誌 v.14 no.2 蜂毒藥鍼刺戟이 3-MCA 誘發 上皮腫에 對한 亢癌 및 免疫反應에 미치는 影響 權奇祿(外)
  20. 大韓韓醫學會誌 v.18 no.1 四君子湯 및 四物湯 藥鍼이 免疫反應에 미치는 影響 李秉烈
  21. 大韓韓醫學會誌 v.16 no.2 金銀花 藥鍼液이 亢癌 및 癌豫防 效果에 미치는 影響 김중완
  22. 大韓韓醫學會誌 v.17 no.3 전갈 약침액의 항돌연변이 및 항암효과 김소형
  23. 大韓韓醫學會誌 v.18 no.3 益智仁 藥鍼이 亢癌 및 免疫機能에 미치는 實驗的 硏究 朴祥鎔
  24. 大韓韓醫學會誌 v.18 no.3 토絲子藥鍼이 亢癌作用 및 免疫效果에 對한 實驗的 硏究 이재복
  25. 大韓韓醫學會誌 v.18 no.1 魚腥草藥鍼이 B16 흑색종 癌모델에 對한 亢腫瘍效果 및 免疫反應에 미치는 影響 배원형(외)
  26. 독성학 김양강
  27. 면역학 김세종
  28. Science v.193 Selective in vitro growth of T lymphocytes from normal human bone marrow Morgan, D.A.;Ruscetti, F.W.;Gallo, R.C. https://doi.org/10.1126/science.181845
  29. 임상병리파일(제3판) 이귀녕(외)