Journal of Sasang Constitutional Medicine (사상체질의학회지)

The Society Of Sasang Constitutional Medicine (사상체질의학회)
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Effects of Yuldahansotang water extract on Cultured Primary Hippocampal Cell Culture Damaged by Hydrogen Peroxide

열다한소탕(熱多寒少湯) 전탕액(煎湯液)이 Hydrogen Peroxide에 의해 손상(損傷)된 배양(培養) 해마신경세포(海馬神經細胞)에 미치는 영향

  • Park, Hye-Sun (Dept. of Sasang Constitutional medicine, College of Oriental Medicine, Wonkwang Univ.) ;
  • Kim, Kyung-Yo (Dept. of Sasang Constitutional medicine, College of Oriental Medicine, Wonkwang Univ.) ;
  • Go, Gi-Deok (Dept. of Sasang Constitutional medicine, College of Oriental Medicine, Wonkwang Univ.) ;
  • Kim, Il-Hwan ;
  • Lee, Jae-Heung (Dept. of Sasang Constitutional medicine, College of Oriental Medicine, Wonkwang Univ.)
  • 박혜선 (원광대학교 한의과대학 사상체질의학교실) ;
  • 김경요 (원광대학교 한의과대학 사상체질의학교실) ;
  • 고기덕 (원광대학교 한의과대학 사상체질의학교실) ;
  • 김일환 (동신대학교 한의과대학 사상체질의학교실) ;
  • 이재흥 (원광대학교 한의과대학 사상체질의학교실)
  • Published : 2002.04.30
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Abstract

To evaluate the effect of Yuldahansotang(YHT) water extract on cultured hippocampal cell was inhibited by hydrogen peroxide, MTT assay, NR assay, Neurofilament enzymeimmuno assay and DNA synthesis assay were carried out after the cultured hippocampal cells were preincubated with various concentrations of YHT water extract for 3 hours prior to exposure of hydrogen peroxide. The results obtained were as follows: 1. Hydrogen Peroxide decreased the survival rate of the cultured hippocampal cells on NR assay and MIT assay. 2. YHT water extract have efficacy of increasing a amount of neurofilament decreased by hydrogen peroxide in cultured hippocampal cells. 3. YHT water extract have efficacy of increasing DNA synthesis decreased by hydrogen peroxide in cultured hippocampal cells. From above the results, It is concluded that YHT has marked efficacy in preventing for the damages by hydrogen peroxide.

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