Angiotensin Converting Enzyme Inhibitory Activity in Peptic Hydrolysates of Cooking Discards from Anchovy Factory Ship

멸치 가공선 자숙폐액 Pepsin 가수분해물의 Angiotensin 전환효소 저해작용

  • Ji, Cheong-Il (Faculty of Food and Biotechnology/Institute of Seafood Science, Pukyong National University) ;
  • Lee, Ji-Hye (Faculty of Food and Biotechnology/Institute of Seafood Science, Pukyong National University) ;
  • Park, Douck-Choun (Faculty of Food and Biotechnology/Institute of Seafood Science, Pukyong National University) ;
  • Gu, Yeun-Suk (Faculty of Food and Biotechnology/Institute of Seafood Science, Pukyong National University) ;
  • Kim, In-Soo (Division of Marine Bioscience/Institute of Marine Industry, Gyeongsang National University) ;
  • Lee, Tae-Gee (Department of Marine Food Industry, Provincial College of Namdo) ;
  • Jung, Kyoo-Jin (Department of Marine Food Industry, Provincial College of Namdo) ;
  • Park, Yeung-Ho (Faculty of Food and Biotechnology/Institute of Seafood Science, Pukyong National University) ;
  • Kim, Seon-Bong (Faculty of Food and Biotechnology/Institute of Seafood Science, Pukyong National University)
  • 지청일 (부경대학교 식품생명공학부/수산식품연구소) ;
  • 이지혜 (부경대학교 식품생명공학부/수산식품연구소) ;
  • 박덕천 (부경대학교 식품생명공학부/수산식품연구소) ;
  • 구연숙 (부경대학교 식품생명공학부/수산식품연구소) ;
  • 김인수 (경상대학교 해양생물이용학부) ;
  • 이태기 (남도대학 해양식품산업과) ;
  • 정규진 (남도대학 해양식품산업과) ;
  • 박영호 (부경대학교 식품생명공학부/수산식품연구소) ;
  • 김선봉 (부경대학교 식품생명공학부/수산식품연구소)
  • Published : 2002.06.01

Abstract

The angiotensin converting enzyme (ACE) inhibitory activity in peptic hydrolysate of raw anchovy cooking discards was 51.3% at 1 mg of protein per $100\;{\mu}L$ sample solution. While, after the treatment of pepsin for 4 h, was 65.8%. The crude peptides fractionated through Bio-gel P-2 column chromatography consisted of five fractions $({P-1}{\sim}{P-5})$ and had maximum inhibitory activity in the fraction P-2 ($IC_{50}$=0.319 mg protein/mL). The fraction P-2 was rich in aspartic acid, glutamic acid, and glycine.

현재 우리나라 남해안에서 많이 가공되고 있는 멸치 자건품은 통상 멸치 가공선에서 어획 즉시 자숙처리를 하여 제조되고 있는데, 이때 가공부산물로써 다량 배출되는 자숙액은 현재로서는 이용가치가 없을 뿐만 아니라 그대로 바다에 폐기되어 해양오염까지 유발시키고 있는 실정이다. 그리하여 이들 멸치 자숙 폐액을 대상으로 기능성 조미소재로서의 이용가치를 살펴보기 위해 소화효소인 pepsin에 의한 가수분해와 겔크로마토그라피를 이용하여 angiotensin 전환효소(ACE)저해 peptide를 검색하고 그 효과를 살펴보았다. 먼저, 멸치 자숙 폐액의 단백질 농도에 따른 ACE 저해작용을 살펴 본 결과 시료 용액 $100\;{\mu}L$당 1 mg으로 하였을 때 51.3%의 저해능을 나타내었다. 한편, pepsin을 이용하여 가수분해할 경우, ACE 저해효과는 4시간째에 65.8%로써 가장 높게 나타내었다. 이 가수분해물을 Bio-gel P-2 column chromatography에 의하여 분리한 결과, $({P-1}{\sim}{P-5})$의 5개의 획분을 얻었고 ACE 저해효과가 가장 우수한 획분은 P-2($IC_{50}$=0.319 mg protein/mL)로 나타났으며 이 획분의 아미노산 조정을 살펴본 결과 aspartic acid, glutamic acid 및 glycine의 함량이 높았다.

Keywords

References

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