한국식품과학회지 (Korean Journal of Food Science and Technology)

한국식품과학회 (Korean Society of Food Science and Technology)
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상용하는 식물성 기름에서 지질과산화의 독성물질 4-hydroxy-2-alkenals 정량

Quantification of 4-Hydroxyalkenals in Oils Consumed in Korea

  • 발행 : 2002.10.01
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초록

한국인이 상용하는 식물성 유지로부터 n-3 지방산과 n-6 지방산 산화의 독성 산물인 4-hydroxy-2-hexenal(HHE)과 4-hydroxy-2-nonenal(HNE)를 높은 감도로 동시 정량 하기 위하여 GC/MS/SIM(m/z = 157)을 이용하여 정량 하고 노출량을 추정하였다. 한국인이 섭취하는 식물성 유지의 98%를 차지하는 4종류의 유지로부터 4-hydroxy-2-alkenals의 총 노출량은 $2.7\;{\mu}g/day$로 나타났고, 시장에서 수거한 튀김 기름의 경우에는 상온에서 보관한 유지류에 비해 높은 양의 HHE와 HNE가 검출되었다. 튀김 과정 중에 형성된 4-hydroxy-2-alkenals는 튀김 음식으로 이행될 가능성이 있으므로, 튀김 음식을 자주 섭취하는 집단은 4-hydroxy-2-alkenals에 대한 노출량이 더 높을 것으로 사료된다. n-3와 n-6 지방산의 생리 활성에 대한 보고로 인하여 이들 고도 불포화 지방산의 섭취가 증가하고 있으므로, 불포화 지방산을 다량 함유하고 있는 식품으로부터 n-3와 n-6 지방산의 특이적 산화 지표인 4-hydroxy-2-hexenal과 4-hydroxy-2-nonenal을 동시 정량 하여 식품 품질 및 안전성 평가를 하는 것은 바람직하다고 생각된다. 본 실험에서 보여 준, 상용 식물성 유지의 4-hydroxy-2-alkenals 함량에 관한 데이터는 식품을 통해 노출된 4-hydroxy-2-alkenals가 인체에 미치는 생리적 효과를 평가하기 위한 앞으로의 연구에 중요한 정보를 제공할 것이다.

4-Hydroxyalkenals are cytotoxic aldehydes generated by the oxidation of n-6 and n-3 polyunsaturated fatty acids. To evaluate the potential risk of 4-hydroxyalkenals on Koreans, quantitative data of various oils are necessary. Simultaneous monitoring of 4-hydroxyhexenal and 4-hydroxynoneal in 39 samples including new and used ones through single ion monitoring mode of GC/MS detected both aldehydes in all samples tested, ranging from 0.21 to 26.9 nmol/g for 4-hydroxy-2-hexenal and 0.06 to 56.6 nmol/g for 4-hydroxy-2-nonenal. Frying oils collected from local markets showed 2.28-7.90 and 8.31-30.5 nmol/g of 4-hydroxyhexenal and 4-hydroxynoneal, respectively. National health and nutrition survey data were employed to determine the exposure effect to these 4-hydroxyalkenals from the four most consumed oils in Korea. Daily exposures to hydroxyalkenals excluding possible exposure from fried food were $1.9\;{\mu}g$ from soybean oil, $0.5\;{\mu}g$ from sesame oil, $0.2\;{\mu}g$ from corn oil, and $0.1\;{\mu}g$ from perilla oil. Due to the increasing consumption of polyunsaturated fatty acids in Korea, these data may provide valuable information for evaluating possible physiological effects of 4-hydroxyalkenals from vegetable oils.

키워드

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