Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Analysis of Melatonin Content from Domestic Edible Plants

국내산 식용식물체의 멜라토닌 함량 분석

  • Kim, Seok-Joong (Department of Food Science and Technology, Catholic University of Daegu)
  • 김석중 (대구가톨릭대학교 식품공학과)
  • Published : 2002.12.01
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Abstract

Melatonin, which is a hormone secreted from pineal gland of brain and known to prevent oxidative damages of various tissues, was analyzed in 26 domestic edible plants. For the preparation of melatonin fraction, 50% ethanol extract prepared from lyophilized plant powder was filtered and applied on TLC plate. Melatonin position on TLC developed with acetone was identified by fluorescence light and extracted with methanol. This methanolic fraction was injected into HPLC comprising ODS-A column, fluorescence detector, and mobile phase consisting of a mixture (30 : 70, v/v) of 70% ammonium acetate and methanol at a flow rate of 1.0 mL/min. Melatonin was identified at the retention time of 17 min. Results revealed that celery, leek, broccoli, and cauliflower had higher melatonin contents than others.

뇌의 송과선에서 분비되는 호르몬인 melatonin은 생체의 산화적 손상을 방어하는 효능이 있다고 알려져 있는데, 26종의 식물체로부터 그 존재 및 함량을 분석하였다. Melatonin 함유 분획을 조제하기 위하여 동결건조된 식물체 분말을 50% ethanol로 추출한 다음 여과 후 TLC 상에서 acetone으로 전개시켜 melatonin을 분리하였다. 형광하에서 확인된 melatonin spot은 methanol로 추출한 다음 HPLC로 분석하였다. HPLC 분석시 ODS-A column을 사용하였으며 이동상은 70% ammonium acetate와 메탄올을 30 : 70(v/v)으로 섞어서 1.0 mL/min의 속도로 흘려주었다. Melatonin peak의 확인은 형광 detector를 이용하였으며 retention time은 17분이었다. 이같은 분석조건에서 셀러리, 부추, 브로콜리, 컬리플라워 등에서 높은 melatonin 함량이 확인되었다.

Keywords

References

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