Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Antioxidative Activities of Ethanol Extract Prepared from Leaves, Seed, Branch and Aerial Part of Crotalaria sessiflora L.

각 부위별 활나물(Crotalaria sessiflora L.) 에탄올 추출물의 항산화효과

  • Published : 2002.12.01
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Abstract

Antioxidative activities of ethanol extracts of seed, branch, leaves, and aerial part of Crotalaria sessiflora L. were compared using in vitro experimental model. Solid contents of each extracts were 12.68, 16.28, 13.04, and 18.59 g/ 100 mL, and phenolic acid contents were $0.082{\pm}0.003,\;0.099{\pm}0.010,\;0.071{\pm}0.002,\;and\;0.094{\pm}0.011\;mg/mL$ in branch, aerial part, seed, and leaves, respectively. Extracts prepared from leaves showed highest electron-donating ability toward DPPH. SOD-liked activities were 78.95, 70.85, 74.65, and 87.49% in aerial part, branch, seed, an leaves respectively. Extracts prepared from leaves showed 59.39% inhibitory effect on peroxidation of egg yolk lecithin.

활나물의 항산화 효과를 측정하기 위하여 잎, 종자, 가지 및 지상부를 부위별로 분류하여 에탄올로 추출한 후 동결 건조한 각 추출물의 항산화 효과를 측정하였다. 각 부위별 추출물의 고형분 함량은 가지 12.68 g/100 mL, 지상부 16.28 g/100 mL, 종자 13.04 g/100 mL, 그리고 잎이 18.59 g/100 mL로 잎의 추출수율이 가장 높았다. 총 페놀함량은 가지 $0.082{\pm}0.003\;mg/mL$, 지상부 $0.099{\pm}0.010\;mg/mL$, 종자 $0.071{\pm}0.002\;mg/mL$, and 잎이 $0.094{\pm}0.011\;mg/mL$로 나타나 지상부의 총 페놀 함량이 높았다. 이들 추출물을 DPPH법으로 free radical 소거능을 측정한 결과 가지 12.29%, 종자 13.18%, 잎 13.76% 및 지상부가 10.82%로 나타났다. SOD 유사활성 측정은 지상부 78.95%, 가지 70.85%, 종자 74.63%, 그리고 잎이 87.49%로 잎 추출물의 활성이 가장 높았다. lecithin 산화 저해율은 잎 $59.39{\pm}1.79%$, 종자 $57.82{\pm}9.27%$, 지상부 $56.39{\pm}2.45%$ 및 가지가 $46.41{\pm}2.25%$로 잎에서 가장 높았고 가지가 가장 낮게 나타났다. 이상의 연구결과 활나물 각 부위별 추출물의 항산화 효과 측정 결과 추출 수율이 높았던 잎에서 활성도 높게 나타나 천연 항산화제로 개발 가능성이 시사되었다.

Keywords

References

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