Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Identification of Germination Properties for the Screening of Gamma-irradiated Beans

방사선 조사된 콩류의 선별 검지를 위한 발아특성 확인

  • Oh, Kyeong-Nam (Detection Laboratory of Irradiated Foods, Korea Atomic Energy Research Institute) ;
  • Kang, Eun-Kyoung (Detection Laboratory of Irradiated foods, Korea Atomic Energy Research Institute) ;
  • Park, Choon-Ran (Department of Foods and Nutrition, Chung-Cheong College) ;
  • Yang, Jae-Seung (Detection Laboratory of Irradiated Foods, Korea Atomic Energy Research Institute)
  • 오경남 (한국원자력 연구소 식품검지실) ;
  • 강은경 (한국원자력 연구소 식품검지실) ;
  • 박춘란 (충청대학 식품영양학과) ;
  • 양재승 (한국원자력 연구소 식품검지실)
  • Published : 2002.12.01
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Abstract

Germination method was used to screen the biological changes in soybean, kidney bean, and red bean caused by gamma irradiation. Beans were irradiated at 0.1, 0.3, 0.5, 0.7, and 1.0 kGy. Ten beans of each sample were placed on moistened cotton and germinated at $30{\circ}C$. The root lengths were measured daily for 5 days. Root lengths of all beans grew continuously for 5 days, but the growth rate of irradiated beans decreased significantly from fourth day. Unirradiated beans showed the highest growth rate during 5 days of germination. Gamma-irradiated beans could be screened by measuring the daily growth rate and root length during germination.

본 연구는 콩류의 방사선 조사 여부를 검지하기 위해서 생물학적 방법인 발아법으로 확인하였다. 콩류는 대두, 강남콩, 그리고 팥으로 0.1, 0.3, 0.5, 0.7, 1.0 kGy의 조사선량으로 방사선 조사하고 5일 동안 뿌리의 길이를 측정하여 방사선 조사에 의한 발아 특성을 살펴보았다. 모든 시료는 5일 동안 지속적인 길이 성장을 하였지만, 방사선 조사에 의해서 길이 성장이 억제되어 발아 3일째 부터는 뿌리길이에 유의적인 차이를 보이지 않았다. 대두와 팥은 발아 2일째, 강낭콩은 발아 3일째 비조사 시료와 조사 시료간에 1 cm 단위의 길이 차이를 보였다. 발아 5일째는 조사선량간에 유의적인 차이를 나타내는 경향이었지만 0.5 kGy 이상 조사 시료간에는 유의적인 길이 차이를 보이지 않았다. 또한 모든 시료에서 4일부터 길이 성장정도가 현저히 낮아지고 발아 5일 동안 비조사 시료의 성장정도가 가장 큰 것으로 나타났다. 콩류의 방사선 조사 여부는 발아 5일 동안 뿌리 길이의 유의적인 길이 성장과 비조사 시료와 조사 시료들의 성장정도를 측정함으로써 방사선 조사 여부의 선별검지가 가능한 것으로 판단되었다.

Keywords

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