Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지

Optimization of Major Culture Elements on Growth and Shikonin Production in the Lithospermum erythrorhizon Hairy Root Culture

  • Hwang, Ok-Jin (Faculty of Biological Sciences, Chonnam National University) ;
  • Kim, Yu-Jeong (Faculty of Biological Sciences, Chonnam National University) ;
  • Sung, Nak-Sul (National Crop Experiment Station, RDA) ;
  • Ahn, Jun-Cheul (Dept. of Life Sciences, Seonam University) ;
  • Kim, Sik-Eung (Dept. of Life Sciences, Seonam University) ;
  • Hwang, Baik (Faculty of Biological Sciences, Chonnam National University)
  • Published : 2002.11.30
Download PDF
⟨ Previous Next ⟩

Abstract

The effects of basal media, carbon, nitrogen, phosphate and some major macro elements on growth and shikonin production in Lithospermum erythrorhizon hairy root culture were studied. Among examined media, growth of hairy root cultured in B5 liquid medium was rapid, whereas shikonin production was high in MS liquid medium. Under B5 basal medium, sucrose concentration for optimal growth and shikonin production was 9% and 4% respectively. The growth and shikonin production on pH changes in B5 medium resulted little effect in pH 5.8 to pH 8.8 ranges, whereas growth was decreased dramatically in both above 8.8 and under 5.8. Nitrogen source and concentration effected on the growth and shikonin production. The highest growth rate was in B5 medium (50 mM $KNO_3$ and 1 mM $NaH_2PO_4)$, whereas the highest shikonin production was in the condition supplemented with 5 mM $KNO_3$ and 10 mM $NaH_2PO_4$.

Keywords

References

  1. Aimin XU, Bellamya AR, Taylor JA (1999) Expression oftranslationally controlled tumor protein is regulated by calcium at both the transcriptional and post-transcriptional level. Biochem. J. 342 : 683-689 https://doi.org/10.1042/0264-6021:3420683
  2. Cho MH, Paik YS, Hahn TR (1999) Physical Stability of Shikonin Derivatives from the Roots of Lithospermum erythrorhizon Cultivated in Korea. J. Agric, Food Chem. 47 : 4117-4120 https://doi.org/10.1021/jf9902853
  3. Fujita Y. Hata Y. Ogino T. Suga C (1981a) Production of shikonin derivatives by cell suspension cultures of Lithospennum erythrorhizon I. Effects of nitrogen sources on the production of shikonin derivatives. Plant Cell Reports 1 : 59-60 https://doi.org/10.1007/BF00269272
  4. Fujita Y, Hara Y, Suga C, Morhmoto T (1981b) Production of shikonin derivatives by cell suspension cultures of Lithospermum erythrorhizon, II. A new medium for the production of shikonin derivatives. Plant Cell Reports 1 : 61-63 https://doi.org/10.1007/BF00269273
  5. Gaisser S, Heide L (1996) Inhibition and regulation of shikonin biosynthesis in suspension cultures of Lithospermum. Phytochenistry41 : 1065-1072 https://doi.org/10.1016/0031-9422(95)00633-8
  6. Heide L, Tabata M (1987) Geranylpyrophosphate : $\rho$-hydeoxybenzoate geranyltransferase acitivity in extracts of Lithospermum erythrorhizon cell cultures. Phytochemistry 26 : 1651-1655 https://doi.org/10.1016/S0031-9422(00)82263-3
  7. Heide L, Nishioka N, Fukui H, Tabata M (1989) Enzymatic regulation of shikonin biosynthesis in Lithospermum erythrorhizon cell cultures. Phytochemistry 28 : 1873-1877 https://doi.org/10.1016/S0031-9422(00)97877-4
  8. Mizukani H, Konoshima M (1977) Effects of nutritional factors on shikonin derivative formation in Lithospermum erythrorhizon callus cultures. Phytochemistiy 16 : 1183-1186 https://doi.org/10.1016/S0031-9422(00)94356-5
  9. Mizukami H, Konoshima M, Tabata M (1978) Variation in pigment production in Lithospermum erythrorhizon callus cultures. Phytochemistry 17 : 95-97 https://doi.org/10.1016/S0031-9422(00)89687-9
  10. Shimomura, K, Lude H, Saga H, Kamada, H (1991) Shikonin production and secretion by hairy root cultures of Lithospermum erythrorhizon. Plant Cell Reports 10 : 282-285
  11. Touno K., Harada K, Yoshimatsu K, Yazaki K, Shimomura K (2000) Histological Observation of Red Pigment Foimed on Shoot Stem of Lithospermum erythrorhizon. Plant Biotechnology. 17(2) : 127-130 https://doi.org/10.5511/plantbiotechnology.17.127