Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Cloning and expression of glutathione S-transferase (GST) cDNA from Gossypium hirsutum L.

  • Kang, Won-Hee (College of Agriculture & Life Science, Kangwon National University) ;
  • Kim, Myong-Jo (College of Agriculture & Life Science, Kangwon National University) ;
  • Lim, Jung-Dae (College of Agriculture & Life Science, Kangwon National University) ;
  • Yun, Song-Joong (Faculty of Biological Resources Sciences, Chonbuk Nat'l Univ.) ;
  • Chung, Ill-Min (Konkuk University) ;
  • Yu, Chang-Yeon (College of Agriculture & Life Science, Kangwon National University)
  • Published : 2002.11.30
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Abstract

A gene coding for the GST of cotton (Gh-5) was cloned into Escherichia coli and experssed. The enzyme remained within the cytoplasm of E. coli. An 696 bp open reading frame was in the 988 base pair fragment of the recombinant plasmid pET-30b(+). The deduced protein sequence consists of 232 amino acids and has a molecular mass of 30235.58 Da. The cloned enzyme conjugated reduced glutathione and 1-chloro-2,4-dinitrobenzene (CDNB). Plant GST cDNA was expressed in microbe and produced polypeptide had function as an enzyme.

Keywords

References

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