대한약침학회지 (Journal of Pharmacopuncture)

  • 제5권1호
  • /
  • Pages.135-151
  • /
  • 2002
  • /
  • 2093-6966(pISSN)
  • /
  • 2234-6856(eISSN)
대한약침학회 (KOREAN PHARMACOPUNCTURE INSTITUTE)
권호 표지
DOI QR코드

DOI QR Code

래복자(萊?子)가 알러지성 천식(喘息) 동물모형(動物模型)에서 면역반응(免疫反應)에 미치는 영향(影響)

Effects of Raphani Semen on Immuno-response in the Mouse Model of allergi Asthma

  • 박기철 (상지대학교 한의과대학 침구학교실) ;
  • 박재영 (상지대학교 한의과대학 침구학교실) ;
  • 박희수 (상지대학교 한의과대학 침구학교실)
  • Park, Ki-Chul (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Sang-Ji University) ;
  • Park, Jai-Young (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Sang-Ji University) ;
  • Park, Hee-Soo (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Sang-Ji University)
  • 발행 : 2002.02.28
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objective: This study was eanied out to investigate the effects of Raphani Semen on immuno-response in the mouse model of allergic asthma. Methods: In this study, BALB/C mice were divided into 6 groups: Normal (Non-treated group), Control (Group with not treated after allergic sensitization and induction by ovalbumin), Treat I (Group with the oral administration of saline after allergic sensitization and induction by ovalbumin), Treat n (Allergic asthma group treated with acupuncture (BL 13)), Treat III (Allergic asthma group treated with the oral administration of Raphani Semen) and Treat lV (Allergic asthma group treated with the herbal-acupuncture of Raphani Semen (BL 13)). The effect on cytokine was assessed by measuring cytokine (lL-2, IL-4, IL-5, IL-10, IL-12, IFN-r) in bronchoalveoar lavage fluid(ELISA). ResuJts : The results obtained as follows: 1. The production of Interleukin-2 was decreased significantly in Treat I group, Treat n group and Treat IV group as compared with Control group. 2. The production of Interleukin-4 was decreased significantly in Treat I group, Treat II group and Treat IV group as compared with Control group. Among them. the production of Interleukin-4 was decreased remarkably in Treat IV group as compared with other groups. 3. The production of Interleukin-5 was decreased significantly in Treat I group and Treat IV group as compared with Control group. 4. The production of Interleukin-10 was decreased significantly in Treat I group and Treat III group as compared with Control group. 5. The production of Interleukin-12 was all decreased significantly in Treat I group, Treat n group, Treat m group and Treat IV group as compared with Control group. 6. The production of Intelferon- showed no significant changes in Treat I group, Treat n group. Treat m group and Treat Ⅳ group as compared with Control group. Conclusion: These results show that the production of Interleukin-4, 5 was decreased significantly in aJlergic asthma group treated with the herbal-acupuncture of Raph Semen (BL 13), It is known that inactivity of Th2 cell constrained the revelation and controlled hypersenstive action. As to this mechanism, it is suggested that the herbal-acupuncture of Raphani Semen(BL 13) constrained the revelation of allergic asthma.

키워드

참고문헌

  1. 李珩九, 鄭昇杞, 東醫肺系內科學, 서울, 아트동방, pp.187-188, 196-201, 1996
  2. 大韓病理學會, 病理學, 서울, 高文社, pp.604-606, 1990
  3. 全國韓醫科大學 本草學敎室, 本草學, 서울, 永林社, pp.373-374, 1992
  4. 申佶求, 申氏本草學 各論, 서울, 壽文社, pp.691, 1988
  5. 全國韓醫科大學 鍼灸.經血學敎室, 鍼灸學(下), 서울, 集文堂, p.1457, 1993
  6. 全國韓醫科大學 鍼灸.經血學敎室, 鍼灸學(上), 서울, 集文堂, pp.478-479, 1993
  7. 朴喜守, 睮穴硏究鍼灸學, 서울, 醫聖堂, pp.122-123, 1996
  8. 權赫星, 鄭熙才, 鄭昇杞, 李珩九, 淸上補下湯이 Allergy 喘息의 呼吸樣相과 氣管粘膜의 好酸球變化에 미치는 影響, 慶熙韓醫大論文集, 22(1), pp.203-214, 1999
  9. 車恩秀, 鄭熙才, 鄭昇杞, 李珩九, 小靑龍湯이 Asthma model 內의 Cytokine에 미치는 影響, 慶熙韓醫大論文集, 23(1), pp.71-86, 2000
  10. 李珩九, 張仁圭, 五拗湯이 咳嗽 喘息에 미치는 影響, 慶熙韓醫大論文集, 5, pp.175-190, 1982
  11. 權純湖, 鄭熙才, 鄭昇杞, 李珩九, 五拗湯이 알레르기 喘息의 呼吸樣相과 氣管組織에 미치는 影響, 大韓韓醫學會誌, 20(2), pp.98-107
  12. 鄭旭, 鄭熙才, 鄭昇杞, 李珩九, 杏仁과 桔梗이 Asthma model 內의 Cytokine IL-4, IL-5, IL-6에 미치는 影響, 大韓韓方內科學會誌, 21(1), pp.31-38, 2000
  13. 崔炳甲, 數種의 止咳平喘 藥材가 氣管支 平滑筋에 미치는 影響, 圓光大學校 大學院, 博士學位 論文, 1998
  14. 朴永桓, 氣觀支 喘息 생쥐 模型에서 麥門冬 藥鍼이 抗알레르기 및 免疫反應에 미치는 影響, 尙志大學校 大學院, 博士學位論文, 2000
  15. 심문경, 박은정, 정규만, 淸肺散이 마우스의 免疫 및 過敏反應에 미치는 影響, 大韓小兒科學會誌, 10(1), pp.265-297, 1997
  16. 김승수, 정희재, 정승기, 이형구, 解表二陳湯이 알레르기 喘息의 呼吸樣相과 氣管組織에 미치는 影響, 大韓韓方內科學會誌, 19(2), pp.59-71, 1999
  17. Zhang YD, Wayne JE, Lamm RK, Albert RK, Chi EY, Henderson WR, Lewis DB, Influence of the Route of Allergen Administration and Genetic Background on the Murine Allergic Pulmonary Response. AM J Respir Crit Care Mes 155: 661-9,1997 https://doi.org/10.1164/ajrccm.155.2.9032210
  18. 이숙영, 송정섭, 氣管支喘息 생쥐 模型에서 Interleukin-12가 氣道炎症에 미치는 影響, 천식 및 알레르기학회지, 19(1), pp.79-90, 1999
  19. 성은미, 이병렬, 赤芍藥 藥針이 關節炎에 미치는 실험적 연구, 大韓鍼灸學會誌, 17(1), pp.223-224, 2000
  20. Zaalberg, a.B., A simple method for detecting single antibody forming cell, Nature, VoI.202..' 1231, 1964 https://doi.org/10.1038/2021231a0
  21. Nowotony, A., Antigen-antibody interactions in basic exercise in immunochemistry, Springer-Verlay Berlin Heidelberg, N.Y., 217-271, 285-287, 1979
  22. 全國醫科大學敎授, 오늘의 診斷 및 治療, 서울, 한우리, p.287, 1999
  23. 大韓病理學會, 病理學, 서울, 高文社, pp.521-523, 2000
  24. 최병휘, 천식의 동물 모델, 천식 및 알레르기 學會誌, 19(1), pp.16-18, 1999
  25. 黃宮수, 本草求眞, 北京, 人民衛生出版社, p.100, 1987
  26. 上海中醫學院, 中草藥學, 香港, 商務印書館香港分館, pp.451-452, 1983
  27. 楊東喜 編, 本草備要解析, 北京, 國興出版社, p.495, 1991
  28. 黃度淵, 方藥合編, 서울, 南山堂, p.254, 1992
  29. 楊繼洲, 鍼灸大成, 서울, 大星文化社, pp.79-88, 107-109, 114, 403-404, 431, 448, 1984
  30. 洪權義, 蔡禹錫, 喘息의 鍼灸治療에 대한 文獻的 考察, 大田大學校 論文集 韓醫學編, 4(2), pp.137-139, 1996
  31. 許浚, 東醫寶鑑, 서울, 大星文化社, pp.283-284, 1992
  32. 王琦, 黃帝內經素問今釋, 서울, pp.122, 126, 439-440, 1983
  33. 이종걸, Allergy性 喘息에 대한 減感作療法의 效果, 忠南大學校 大學院, 대전, 碩士學位論文, 1993
  34. 유욱상, 麥門冬湯이 알레르기 喘息의 呼吸樣相과 氣管組織에 미치는 影響, 서울, 慶熙大學校 大學院, 碩士學位論文, 1999
  35. 왕중권, 定喘湯이 알레르기성 喘息의 呼吸 樣相과 氣管粘膜의 好酸球浸潤에 미치는 影響, 서울, 慶熙大學校 大學院, 碩士學位論文, 1999
  36. 서창훈, 麥門冬湯加味方이 實驗的 알레르기 喘息에 미치는 影響, 부산, 東義大學校 大學院, 碩士學位論文, 2001
  37. 이준우, 小靑龍湯이 알레르기 喘息 모델 흰쥐의 BALF내 免疫細胞에 미치는 影響, 서울, 慶熙大學校 大學院, 碩士學位論文, 2001
  38. 이상준, 定喘化痰降氣湯이 實驗的 喘息의 組織學的 變化에 미치는 影響, 대구, 慶山大學校 大學院, 碩士學位論文, 2001
  39. 김진주, 麥門冬湯과 定喘化痰降氣湯이 알레르기 喘息 모델 흰쥐의 BALF내 免疫細胞 및 血淸 IgE에 미치는 影響, 서울, 慶熙大學校 大學院, 2001
  40. 서울大學校 醫科大學 編, 免疫學, 서울, 서울大學校 出版部, pp.2-3, 72-5, 136-7, 1994
  41. 김세종, 免疫學, 서울, 고려의학, pp.259-265, 1994
  42. 全南大學校 醫科大學 면역 및 알레르기학 편찬위원회 編, 면역 및 알레르기학, 광주, pp.158-177, 1999
  43. 하대유, 그림으로 본 免疫學, 서울, 高文社, pp.282-283, 294-296, 1994
  44. 菊地浩吉, 最新免疫學, 서울, 集文堂, pp.1-35, 1993
  45. Larsen GL, Late- hase reactions Observation on pathogenesis andprevention, J Allergy ClinImmunol, 11, 665, 1985
  46. Poston RN, Chanez, Immunohistochemical characterization of the cellular infiltration in asthmatic bronchi, AM Rev Resir Dis, 145,918,1992 https://doi.org/10.1164/ajrccm/145.4_Pt_1.918
  47. Lopez AF, Sanderson CJ, Gamble JR, Campbell HR, Young IG, Vadas MA, Recombinant human Interleukin-5 is a selective activator of human eosinophil function, J Exp Med 167, 219-24, 1988 https://doi.org/10.1084/jem.167.1.219
  48. Ryan JJ, Interleukin-4 and its receptor, Essential mediators of the allergic response, J Allergy Clin Immunol 99, 1-5, 1997 https://doi.org/10.1016/S0091-6749(97)70293-8|
  49. Robinson DS, Hamid Q, Ying S, Tsicopoulos A, Barkans J, Bentley AM, Corrigan C, Durham SR, Kay AB, Predominant Th2-like bronchoalveolar TIymphocite population in atopic asthma, N Engl J Med 326,298-304, 1992 https://doi.org/10.1056/NEJM199201303260504
  50. Borish L, Rosenwasser LJ, Update oncytokine, J Allergy Clin Immunol 97, 719-33, 1996 https://doi.org/10.1016/S0091-6749(96)80146-1
  51. Gavett SH, O'Heam DJ, Li X, Huang SK, Finkehnan FD, Wills-Karp M, Interleukin-12 inhibits antigeninduced airway hyperresponsiveness, inflamation, and Th2 cytokine expression in mice, J Exp Med 182, 1527-36,1995 https://doi.org/10.1084/jem.182.5.1527
  52. Sur S, Lam J, Bouchard P, Sigounas A, Holbert D, Metzger WJ, Immunomodu1artory effects of IL-12 on allergic lung inflamation depend on timing of doses, J Immunol 157, 4173-80,1996