Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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Shenqi-wan Increases Cell Proliferation of Cultured Hippocampal Cell Line HiB5 and Dentate Gyrus of Young Sprague-Dawley Rats

신기환 약침이 HiB5 해마세포와 어린 Sprague-Dawley계 흰쥐의 치상회 세포생성에 미치는 영향

  • Kim Youn-Jung (Dept. of Physiology, College of Medicine, Kyung Hee University) ;
  • Jang Mi-Hyun (Dept. of Physiology, College of Medicine, Kyung Hee University) ;
  • Shin Min-Chul (Dept. of Physiology, College of Medicine, Kyung Hee University) ;
  • Lim Baek-Vin (Dept. of Physiology, College of Medicine, Kyung Hee University) ;
  • Chung Joo-Ho (Dept. of Pharmacology, College of Medicine, Kyung Hee University) ;
  • Bahn Gyun-Ho (Dept. of Psychiatry, College of Medicine, Kyung Hee University) ;
  • Paik Eun-Kyung (Gochun Oriental Medical Clinic) ;
  • Park Jae-Hyung (Gochun Oriental Medical Clinic) ;
  • Kim Ee-Hwa (Dept. of Meridian & Acupoint, College of Oriental Medicine, Semyung University) ;
  • Kim Chang-Ju (Dept. of Physiology, College of Medicine, Kyung Hee University)
  • 김연정 (경희대학교 의과대학 생리학교실) ;
  • 장미현 (경희대학교 의과대학 생리학교실) ;
  • 신민철 (경희대학교 의과대학 생리학교실) ;
  • 임백빈 (경희대학교 의과대학 생리학교실) ;
  • 정주호 (경희대학교 의과대학 약리학교실) ;
  • 반건호 (경희대학교 의과대학 정신과학교실) ;
  • 백은경 (고천한의원) ;
  • 박재형 (고천한의원) ;
  • 김이화 (세명대학교 한의과대학 경혈학교실) ;
  • 김창주 (경희대학교 의과대학 생리학교실)
  • Published : 2002.03.27
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Abstract

목적 : 소아의 정신적 및 신체적 발달지연에 사용되고 있는 신기환이 해마의 신경세포 생성에 미치는 영향을 관찰하고자 하였다. 방법 : 신기환이 세포생성에 미치는 영향을 알아보기 위하여 해마 세포인 HiB5에 신기환을 처리, 배양하여 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay를 통해서 세포생성을 관찰하였다. 또한 3주령의 Sprague-Dawley (S-D)계 흰쥐의 중완혈에 신기환 약침을 자침후 해마의 치상회에서 bromodeoxyuridine (BrdU) immunohistochemistry를 시행하여 세포생성을 관찰하였다. 결과 : HiB5 배양세포에서는 신기환을 처리시 배양시간과 농도별로 세포생성율이 증가하였다. 또한, 대조군의 치상회에서 BrdU 양성세포수는 $128.50{\pm}9.53$, 1 mg/kg 신기환 자침군은 $157.08{\pm}10.82$ 및 10 mg/kg 신기환 자침군은 $204.80{\pm}17.68$로 나타났다. 이러한 결과로 신기환은 어린 S-D계 흰쥐의 치상회에서 세포생성을 증가시키는 것으로 사려된다.

Keywords

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