DOI QR코드

DOI QR Code

Effects of Dietary Glutamine and Glutamate Supplementation on Small Intestinal Structure, Active Absorption and DNA, RNA Concentrations in Skeletal Muscle Tissue of Weaned Piglets during d 28 to 42 of Age

  • Liu, Tao (Key Laboratory of Pig Genetics and Breeding, Ministry of Agriculture) ;
  • Peng, Jian (Key Laboratory of Pig Genetics and Breeding, Ministry of Agriculture) ;
  • Xiong, Yuanzhu (Key Laboratory of Pig Genetics and Breeding, Ministry of Agriculture) ;
  • Zhou, Shiqi (Department of Animal Science, Huazhong Agricultural University) ;
  • Cheng, Xuehui (Key Laboratory of Pig Genetics and Breeding, Ministry of Agriculture)
  • Received : 2001.06.07
  • Accepted : 2001.08.30
  • Published : 2002.02.01

Abstract

Seventy-four piglets were used to investigate the effects of dietary glutamine (Gln) and glutamate (Glu) on the mucosal structure and active absorption of small intestinal, DNA and RNA concentrations of skeletal muscle tissue in piglets during d 28 to 42 of age. Postweaning piglets were fed for 14 d corn- and soybean meal-based diets supplemented with 0.0 or 1.0% L-Gln or L-Glu. On d 7 and 14 postweaning, pigs' small intestinal sections and longissimus dorsi were collected, at the same time, the D-xylose absorption test was conducted. The results suggested that in comparison to control piglets, jejunal atrophy during the first week postweaning was prevented by the glutamine and glutamate supplementation (1%) and the capability of small intestine to absorb Dxylose was improved. Furthermore the RNA concentration in skeletal muscle tissue was increased. These results provide an experimental basis for use of glutamine and glutamate on alleviating the weaning stresses and improving piglets' growth performance.

Keywords

References

  1. Alverdy, A. M. 1990. Effects of glutamine-supplemented diets on immunology of the gut. J. P. E. N. 14:109s-113s.
  2. Ardawi, M. S. M. 1988. Glutamine and glucose metabolism in human peripheral lymphocytes. Metabolism 37:99-103. https://doi.org/10.1016/0026-0495(88)90036-4
  3. Brand, K., W. Fekl, J. von Hintenstern, K. Langer, P. Luppa and C. Schoemer. 1989. Metabolism of glutamine in lymphocytes. Metabolism 38:29-33. https://doi.org/10.1016/0026-0495(89)90136-4
  4. Castell, L. M. and E. A. Newsholme. 2001. The relation between glutamine and the immunodepression observed in exercise. Amino Acids. 20:49-61.
  5. Grant, J. 1988. Use of L-glutamine in total parenteral nutrition. J. Surg. Res. 44(5):506. https://doi.org/10.1016/0022-4804(88)90155-2
  6. Hamilton, P. B. 1945. Glutamine: a major constituent of free.$\alpha$-amino acids in animal tissues and blood plasma. J. Biol. Chem. 158:397-409.
  7. Hampson, D. J. 1986. Alterations in piglet small intestinal structure at weaning. Res. in Vet. Sci. 40:32-40.
  8. Helton, W. S. 1994. The pathophysiologic significance of alterations in intestinal permeability induced by total parenteral nutrition and glutamine. J. P. E. N. 18:289.
  9. Hetty, M. G., Van Beers-Schreurs, J. A. Marias Nabuurs, Liebe Vellenga, J. Hilda and Kalsbeek-Van der Valk. 1998. Weaning and the weaning diet Influence the villous height and crypt depth in small intestine of pigs and alter the concentration of short-chain fatty acids in the large intestine and blood. J. Nutr. 128:947-953.
  10. Kapadia, C. R., M. F. Colpoys, Z. M. Jiang, D. J. Johnson, R. J. Smith and D. W. Wilmore. 1985. Maintenance of skeletal muscle intracellular glutamine during standard surgical trauma. J. P. E. N. 9:583.
  11. Klasing, K. C. and R. E. Austic. 1984. Changes in protein degradation in chicken due to an inflammatory challenge. Pro. Soc. Eep. Biol. Med. 176:292-296.
  12. Klasing, K. C. and R. E. Austic. 1984. Changes in protein synthesis due to an inflammatory challenge. Pro. Soc. Eep. Biol. Med. 176:285-291. https://doi.org/10.3181/00379727-176-41872
  13. Klasing, K. C., D. E. Laurin, D. K. Peng and D. M. Fry. 1987. Immunologically mediated growth depression in chicken influence of feed intake, corticosterone and interleukin-1. J. Nutr. 117:629-1637.
  14. Lewis, S. A., J. R. Berg and T. J. Kleine. 1995. Modulation of epithelial permeability by extracellular macromolecules. Physiol. Rev. 75:561.
  15. Li, Y. SH., Fangnan Liu, Xueqin Sheng, Jieshou Li. 1995. Testing and implication of D-xylose in serum and urine. Shannxi Medical Testing. 10:2-4.
  16. Li, J., B. Langkamp-Henken, K. Suzuki and L. H. Stahlgren. 1994. Glutamine prevents parenteral nutrition-induced increases in intestine permeability. J. P. E. N. 18:303-307.
  17. Liu Tao and Jian Peng. 1999. Effects of glutamine and glutamate on the performance of early-Weaned piglets. Journal of Huazhong Agricultural University. 18(5):457-460.
  18. Miller, B. G., T. J. Newby, C. R. Stokes, D. J. Hampson, P. J. Brown and F. J. Boukne. 1984. The Importance of dietary antigen in the cause of postweaning diarrhea in Pigs. Am. J. Vet. Res. 45:9.
  19. Millward, D. J., M. M. Jepson and A. Omer. 1989. Muscle glutamine concentration and protein turnover in vivo in malnutrition and in endotoxemia. Metabolism 38(Suppl. 1):6. https://doi.org/10.1016/0026-0495(89)90132-7
  20. Neame, K. D. and G. Wiseman. 1957. The transamination of glutamic and aspartic acids during absorption by the small intestine of the dog in vivo. J. Physiol. 135:442-450. https://doi.org/10.1113/jphysiol.1957.sp005722
  21. Newsholme, E. A. and B. M. Parry. 1990. Properties of glutamine release from muscle and its importance for the immune system. J. P. E. N. 14:63s-67s.
  22. O'Dwyer, S. T., R. J. Smith, T. L. Hwang and D. W. Wilmore. 1989. Maintenance of small bowel mucosa with glutamineenriched parenteral nutrition. J. P. E. N. 13:579.
  23. Parry-Billings, M., E. Blomstrand, N. McAndrew and E. A. Newsholme. 1990. A communication link between skeletal muscle, brain, and cells of the immune system. In J. Sports med. 11:122-128. https://doi.org/10.1055/s-2007-1024863
  24. Reeds, P. J., D. G. Burrin, B. Stoll and F. Jahoor. 2000. Intestinal glutamate metabolism. J. Nutr. 130:978s-982s.
  25. Reeds, P. J., D. G. Burrin, B. Stoll, F. Jahoor, L. Wykes, J. Henry and M. E. Frazer. 1997. Enteral glutamine is the preferential precursor for mucosal glutathione synthesis in the piglet. Am. J. Physiol. 273:E408-E415.
  26. Reeds, P. J., L. J. Wykes, J. E. Henry, M. E. Frazer, D. G. Burrin and F. Jahoor. 1996. Enteral glutamate is almost completely metabolized in first pass by the gastrointestinal tract of infant pigs. Am. J. Phyisol. 220:E413-E418.
  27. Rhoads J. M., E. O. Keko and J. P. Woodard. 1992. L-Glutamine and D-glucose stimulate oxidative metabolism and NaCl absorption in piglet jejunum. Am. J. Physiol. 10(3):683-691.
  28. Rhoads, J. M., W. Chen, D. Chu, H. M. Berschneider, R. A. Argenzio and A. M. Parodiso. 1994. L-Glutamine and Lasparagine stimulate $Na^+$/$H^+$ exchange in porcine jejunal enterocytes. Am. J. Physiol. 266:G828-G838.
  29. Rhoads, J. M., E. O. Keku, J. Qujnn, J. Woosely and J. G. Lecce. 1991. L-Glutamine stimulates jejunal sodium and chloride absorption pig rotavirus enteritis. Gastroenterology 100:83-691.
  30. Salloum, R. M., K. A. Herskowitz and W. W. Souba. 1990. Intravenous glutamine stimulates brush border gut glutamine transport. Surg. Forum. 40:195.
  31. SAS. 1990. SAS User's Guide: Statistics, SAS Inst., Inc., Cary, NC.
  32. Smith, R. and K. Panico. 1985. Automated analysis of ophthalaidehyde derivatives of amino acids in physiological fluids by reverse high performance liquid chromatography. J. Lip. Chromatogram. 8:1783-1788. https://doi.org/10.1080/01483918508074095
  33. Souba, W. W., V. S. Klimberg and D. A. Plumley. 1990. The role of glutamine in maintaining a healthy gut and supporting the metabolic response to injury and infection. J. Sury. Res. 48: 383-329. https://doi.org/10.1016/0022-4804(90)90080-L
  34. Souba, W. W., R. J. Smith and D. W. Wilmore. 1985. Effect of glucocorticoids on glutamine metabolism in visceral organs. Metabolism 34:450. https://doi.org/10.1016/0026-0495(85)90211-2
  35. Tong M, B., A. G. Laarrveld, D. L Van Kessel, A. Hamilton and J. F. P. Estrada. 1999. Effect of segregated early weaning on postweaning small intestinal development in Pigs. J. Anim. Sic. 1077:3139-3200.
  36. Vinnars, E. 1975. Influence of the postoperative state on the intracellular free amino acid in human muscle tissue. Ann. Sury. 182:665. https://doi.org/10.1097/00000658-197512000-00001
  37. Windmueller, H. G. and A. E Spaeth. 1980. Respiratory fuels and nitrogen metabolism in vivo in small intestine of fed rats. J. Biol. Chem. 255:107-112.
  38. Windmueller, H. G. 1982. Glutamine utilization by the small intestine. Adv. Enzymol. 53:202.
  39. Wu, G. and D. A. Knabe. 1994. Free and protein-bound amino acids in sow's colostrum and milk. J. Nutr. 124:415-424.
  40. Wu, G., S. A. Meier and D. A. Knabe. 1996. Dietry glutamine supplementation prevents jejunal atrophy in weaned pigs. J. Nutr. 126:2578-2584.
  41. Yoo, S. S., C. J. Field and M. I. McBurney. 1997. Glutamine supplementation maintains irtramuscular glutamine concentrations and normalizes lymphocyte function in infected early weaned pigs. J. Nutr. 127:2253-2259.
  42. Zhu, J., K. M. Cao, R. Q. Zhou, W. Ch. Cai and H. J. Yuan. 1981. Biochemistry Experiment. 1st Ed. Science and Technology. Press, Shanghai, China. 133-139.

Cited by

  1. Rôles of glutamine and nucleotides in combination in growth, immune responses and FMD antibody titres of weaned pigs vol.75, pp.03, 2002, https://doi.org/10.1017/S1357729800053157
  2. Glutamina, nucleotídeos e plasma suíno em rações para leitões desmamados vol.39, pp.3, 2010, https://doi.org/10.1590/S1516-35982010000300010
  3. Small intestine development in chicks after hatch and in pigs around the time of weaning and its relation with nutrition: A review vol.62, pp.1, 2012, https://doi.org/10.1080/09064702.2012.676061
  4. Inclusion of glutamine associated with glutamic acid in the diet of piglets weaned at 21 days of age vol.15, pp.4, 2014, https://doi.org/10.1590/S1519-99402014000400013
  5. GLUTAMIC ACID IMPROVES BODY WEIGHT GAIN AND INTESTINAL MORPHOLOGY OF BROILER CHICKENS SUBMITTED TO HEAT STRESS vol.17, pp.3, 2015, https://doi.org/10.1590/1516-635x1703355-362
  6. Dietary glutamine supplementation enhances expression of ZO-1 and occludin and promotes intestinal development in Min piglets pp.1651-1972, 2017, https://doi.org/10.1080/09064702.2017.1333133
  7. Effect of glutamine and spray-dried plasma on growth performance, small intestinal morphology, and immune responses of Escherichia coli K88+-challenged weaned pigs1,2 vol.83, pp.3, 2005, https://doi.org/10.2527/2005.833634x
  8. l-Glutamate nutrition and metabolism in swine vol.50, pp.11, 2018, https://doi.org/10.1007/s00726-018-2634-3
  9. Timing carbon turnover (δ13C) in weaned piglet’s brain by IRMS vol.90, pp.2 suppl 1, 2018, https://doi.org/10.1590/0001-3765201820170861
  10. Dietary requirements of synthesizable amino acids by animals: a paradigm shift in protein nutrition vol.5, pp.1, 2014, https://doi.org/10.1186/2049-1891-5-34
  11. Effects of Lactitol and Tributyrin on Growth Performance, Small Intestinal Morphology and Enzyme Activity in Weaned Pigs vol.19, pp.10, 2002, https://doi.org/10.5713/ajas.2006.1470
  12. Effects of Biocom as a Replacement of Glutamine on Performance and Blood Biochemical Indexes of Early Weaned Piglets vol.19, pp.6, 2006, https://doi.org/10.5713/ajas.2006.872
  13. Effect and Mechanism of Glutamine on Productive Performance and Egg Quality of Laying Hens vol.23, pp.8, 2002, https://doi.org/10.5713/ajas.2010.90611
  14. Dietary supplementation with monosodium glutamate is safe and improves growth performance in postweaning pigs vol.44, pp.3, 2002, https://doi.org/10.1007/s00726-012-1420-x
  15. Supplementation of diets with glutamine and glutamic acid attenuated the effects of cold stress on intestinal mucosa and performance of weaned piglets vol.59, pp.10, 2002, https://doi.org/10.1071/an17630
  16. Effects of oral monosodium glutamate administration on serum metabolomics of suckling piglets vol.104, pp.1, 2020, https://doi.org/10.1111/jpn.13212
  17. Free Amino Acids in Human Milk: A Potential Role for Glutamine and Glutamate in the Protection Against Neonatal Allergies and Infections vol.11, pp.None, 2020, https://doi.org/10.3389/fimmu.2020.01007
  18. Functional Amino Acids in Pigs and Chickens: Implication for Gut Health vol.8, pp.None, 2021, https://doi.org/10.3389/fvets.2021.663727