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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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A Method for Evaluation of the Quality of DNA Microarray Spots

  • Zhang, Bao (Department of Biochemistry, First Military Medical University) ;
  • Ma, Wen-Li (Department of Biochemistry, First Military Medical University) ;
  • Hu, Zi-You (Department of Biochemistry, First Military Medical University) ;
  • Shi, Rong (Department of Biochemistry, First Military Medical University) ;
  • Song, Yan-Bin (Department of Biochemistry, First Military Medical University) ;
  • Zheng, Wen-Ling (Liuhuaqiao Hospital)
  • Published : 2002.09.30
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Abstract

To establish a method to evaluate the quality of the printed microarray and DNA fragments' immobilization. The target gene fragments that were made with the restriction display PCR (RD-PCR) technique were printed on a superamine modified glass slide, then immobilized with UV cross-linking and heat. This chip was hybridized with universal primers that were labeled with cy3-dUTP, as well as cDNA that was labeled with cy3-dCTP, as the conventional protocol. Most of the target gene fragments on the chip showed positive signals, but the negative control showed no signal, and vice versa. We established a method that enables an effective evaluation of the quality of the microarrays.

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References

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