BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
권호 표지
DOI QR코드

DOI QR Code

Identification and Characterization of a Conserved Baculoviral Structural Protein ODVP-6E/ODV-E56 from Choristoneura fumiferana Granulovirus

  • Published : 2002.11.30
Download PDF
⟨ Previous Next ⟩

Abstract

A gene that encodes a homologue to baculoviral ODVP-6E/ODV-E56, a baculoviral envelope-associated viral structural protein, has been identified and sequenced on the genome of Choristoneura fumiferana granulovirus (ChfuGV). The ChfuGV odvp-6e/odv-e56 gene was located on an 11-kb BamHI subgenomic fragment using different sets of degenerated primers, which were designed using the results of the protein sequencing of a major 39 kDa structural protein that is associated with the occlusion-derived virus (ODV). The gene has a 1062 nucleotide (nt) open-reading frame (ORF) that encodes a protein with 353 amino acids with a predicated molecular mass of 38.5 kDa. The amino acid sequence data that was derived from the nucleotide sequence in ChfuGV was compared to those of other baculoviruses. ChfuGV ODVP-6E/ODV-E56, along with othe baculoviral ODVP-6E/ODV-E56 proteins, all contained two putative transmembrane domains at their C-terminus. Several putative N-and O-glycosylation, N-myristoylation, and phosphorylation sites were detected in the ChfuGV ODVP-6E/ODV-E56 protein. A similar pattern was detected when a hydrophobicity-plots comparison was performed on ChfuGV ODVP-6E/ODV-E56 with other baculoviral homologue proteins. At the nucleotide level, a late promoter motif (GTAAG) was located at -14 nt upstream to the start codon of the GhfuGV odvp-6e/odv-e56 gene. a slight variant of the polyadenylation signal, AATAAT, was detected at the position +10 nt that is downstream from the termination signal. A phylogenetic tree for baculoviral ODVP-6E/ODV-E56 was constructed using a maximum parsimony analysis. The phylogenetic estimation demonstrated that ChfuGV ODVP-6E/ODV-E56 is most closely related to those of Cydia pomonella granulovirus (CpGV) and Plutella xylostella granulovirus (PxGV).

Keywords

References

  1. Altshul, S. E., Gish, W., Miller, W., Meyers, E. W. and Lipman, D. J. (1990) Basic local alignment search tool. J. Mol. Biol. 215, 403-410. https://doi.org/10.1016/S0022-2836(05)80360-2
  2. Bah, A., Bergeron, J., Arella, M., Lucarotti, C. J. and Guertin, C. (1997) Identification and sequence analyses of the granulin gene of Choristoneura fumiferana granulovirus. Arch. Virol. 142, 1577-1584. https://doi.org/10.1007/s007050050181
  3. Bairoch, A., Bucher, P. and Hofmann, K. (1997) The PROSlTE database, its status in 1997. Nucleic Acids Res. 25, 217-221. https://doi.org/10.1093/nar/25.1.217
  4. Braunagel, S. C., He, H., Ramamurthy, P. and Summers, M. D. (1996) Transcription, translation, and cellular localization of three Autographa califomica nuclear polyhedrosis virus structural proteins: ODV-EI8. ODV-E35, and ODV-EC27. Virology 222, 100-114. https://doi.org/10.1006/viro.1996.0401
  5. Braunugel. S. C. and Summers, M. D. (1994) Autographa caltfornica nuclear polyhedrosis virus, PDV, and ECV viral envelopes and nucleocapsids: structural proteins, antigens, lipid and fatty acid profiles. Virology 202, 315-328. https://doi.org/10.1006/viro.1994.1348
  6. Darvey, J. (1989) Sorting out the secretory pathway. Bioassays 11, 185-187. https://doi.org/10.1002/bies.950110607
  7. Federici, B. A. (1999) Naturally occurring baculoviruses for insect pest control. Methods in Biotechnology 5, 301-305.
  8. Forte, A. J., Guertin, C. and Cabana, J. (1999) Pathogenicity of a granulovirus towards Choristoneura fumiferana. Canadian Entomologist 131. 725-727. https://doi.org/10.4039/Ent131725-6
  9. Funk, C. J. and Consigli, R. A. (1993) Temporal expression and immunogold localization of Plodia interpunctella granulosis virus structural proteins. Virus Res. 28, 57-66. https://doi.org/10.1016/0168-1702(93)90089-6
  10. Garnier, J., Gibrat, J. -F. and Robson, B. (1996) GDR secondary structure prediction method version IV; in Methods in Enzymology vol. 266. Doolittle, R. F. (ed), pp. 540-553, Academic Press. San Diego, Carlifornia. https://doi.org/10.1016/S0076-6879(96)66034-0
  11. Hanllen, J. E., Lund, O., Tolstrup, N., Gooley, A. A., Williams, K. L. and Brunak. S. (1998) NetOglyc: prediction of mucin type O-glycosylation sites based on sequence context and surface accessibibty.Glycoconj. J. 15, 115-130. https://doi.org/10.1023/A:1006960004440
  12. Hofmann, K. and Stoffel, W (1993) TMbase-A database of membrane spanning proteins segments. Biol. Chem. Hoppe-Seyler 374, 166.
  13. Hong, T., Braunagel, S. C. and Summers, M. D. (1994) Transcription, translation, and cellular localization of PDV-E66: a structural protein of the PDV envelope of Autographa califomica nuclear polyhedrosis virus. Virology 204, 210-222. https://doi.org/10.1006/viro.1994.1525
  14. Kozak, M. (1986) Point mutations define a sequence flanking the AUG initiator codon that modulate translation by eukaryotic ribosomes. Cell 44, 282-292.
  15. Kuzio, J., Jaques, R. and Faulkner, P. (1989) Identification of p74, a gene essential for virulence of baculovirus occlusion bodies. Virology 173, 759-763. https://doi.org/10.1016/0042-6822(89)90593-X
  16. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680- 685 https://doi.org/10.1038/227680a0
  17. Luque, T., Finch, R., Crook, N., O'Reilly, D. R. and Winstanley, D. (2001) The complete sequence of the Cydia pomonella granulovirus genome. J. Gen. Viral. 82, 2531-2547 https://doi.org/10.1099/0022-1317-82-10-2531
  18. Moller, S., Croning, M. D. R. and Apweiler, R. (2001) Evaluation of methods for the prediction of membrane spanning regions. Bioinformatics 17, 646-653. https://doi.org/10.1093/bioinformatics/17.7.646
  19. Rohrmann, G. F. (1992) Baculovirus structural proteins, J. Gen. Virol. 73, 749-761. https://doi.org/10.1099/0022-1317-73-4-749
  20. Russell, R. L. and Rormann, G.F. (1993) A 25-kDa protein is associated with the envelopes of occluded baculovirus virions. Virology 195, 532-540. https://doi.org/10.1006/viro.1993.1404
  21. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989) Molecular cloning: a laboratory manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
  22. Swofford, D. L. (2000) PAUP Version 4, Sinauer Associates, Sunderland, Massachusetts.
  23. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. and Higgins, D. G. (1997) The ClustalX windows interface: tlexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 24, 4876-4882.
  24. Whitford, M. and Faulkner, P. (1993) Nucleotide sequence and transcriptional analysis of a gene encoding gp41, a structural glycoprotein of the baculovirus Autographa californica nuclear polyhedrosis virus. J Virol. 67. 2427.

Cited by

  1. Penetration of baculoviruses into cell: Universal mechanism and intriguing details vol.24, pp.2, 2009, https://doi.org/10.3103/S0891416809020013
  2. ODV-Associated Proteins of thePieris rapaeGranulovirus vol.10, pp.6, 2011, https://doi.org/10.1021/pr2000804
  3. Location and phylogenetic analysis of the region immediately upstream of the granulin gene of the Clostera anachoreta granulovirus vol.121, pp.1, 2006, https://doi.org/10.1016/j.virusres.2005.10.005
  4. Transcription, Translation, and Immunolocalization of ODVP-6E/ODV-E56 and p74 Proteins: Two Highly Conserved ODV-associated Envelope Proteins of Choristoneura fumiferana Granulovirus vol.38, pp.1, 2005, https://doi.org/10.5483/BMBRep.2005.38.1.065
  5. Polydnavirus hidden face: The genes producing virus particles of parasitic wasps vol.101, pp.3, 2009, https://doi.org/10.1016/j.jip.2009.04.006
  6. Identification, transcriptional and phylogenetic analysis of the DNA polymerase gene of Pieris rapae granulovirus vol.34, pp.3, 2007, https://doi.org/10.1007/s11262-006-0020-9
  7. Choristoneura fumiferana Granulovirus p74 Protein, a Highly Conserved Baculoviral Envelope Protein vol.36, pp.5, 2003, https://doi.org/10.5483/BMBRep.2003.36.5.475
  8. Choristoneura fumiferana Granulovirus pk-1: A Baculoviral Protein Kinase vol.38, pp.4, 2005, https://doi.org/10.5483/BMBRep.2005.38.4.457