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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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cDNA Microarray Analysis of the Differential Gene Expression in the Neuropathic Pain and Electroacupuncture Treatment Models

  • Ko, Je-Sang (Asan Institute for Life Sciences, University of Ulsan College of Medicine) ;
  • Na, Doe-Sun (Department of Biochemistry, University of Ulsan College of Medicine) ;
  • Lee, Young-Han (Department of Biochemistry and Molecular Biology, College of Medicine, Yeungnam University) ;
  • Shin, Soon-Young (Department of Biochemistry and Molecular Biology, College of Medicine, Yeungnam University) ;
  • Kim, Ji-Hoon (Department of East-West Medicine, Graduate School, Kyung Hee University) ;
  • Hwang, Byung-Gil (Department of East-West Medicine, Graduate School, Kyung Hee University) ;
  • Min, Byung-Il (Department of East-West Medicine, Graduate School, Kyung Hee University) ;
  • Park, Dong-Suk (Department of Acupuncture and Moxibustion, College of Oriental Medicine, Kyung Hee University)
  • Published : 2002.07.31
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Abstract

Partial nerve injury is the main cause of neuropathic pain disorders in humans. Acupuncture has long been used to relieve pain. It is known to relieve pain by controlling the activities of the autonomic nervous system. Although the mechanism of neuropathic pain and analgesic effects of electroacupuncture (EA) have been studied in a rat model system, its detailed mechanism at the molecular level remains unclear. To identify genes that might serve as either markers or explain these distinct biological functions, a cDNA microarray analysis was used to compare the expression of 8,400 genes among three sample groups. Messenger RNAs that were pooled from the spinal nerves of 7 normal. 7 neuropathic pain, and 7 EA treatment rat models were compared. Sixty-eight genes were differentially expressed more than 2-fold in the neuropathic rat model when compared to the normal, and restored to the normal expression level after the EA treatment. These genes are involved in a number of biological processes, including the signal transduction, gene expression, and nociceptive pathways. Confirmation of the differential gene expression was performed by a dot-blot analysis. Dot-blotting results showed that the opioid receptor sigma was among those genes. This indicates that opioid-signaling events are involved in neuropathic pain and the analgesic effects of EA. The potential application of these data include the identification and characterization of signaling pathways that are involved in the EA treatment, studies on the role of the opioid receptor in neuropathic pain, and further exploration on the role of selected identified genes in animal models.

Keywords

References

  1. Basbaum, A J. and Fields, H. L. (1984) Endogenous pain control systems. Annu. Rev. Neurosci. 7, 309-338. https://doi.org/10.1146/annurev.ne.07.030184.001521
  2. Bing, Z., Villenueva, L. and Le Bars, D. (1990) Acupuncture and diffuse noxious inhibitory controls: naloxone-reversible depression of activities of trigeminal convergent neurons. Neurosci. 37, 809-818 https://doi.org/10.1016/0306-4522(90)90110-P
  3. Bogoyevitch, M. A, Thien, M. and Ng, D. C. H. (2001) Characterization of protein kinases activated during treatment of cells with okadaic acid. J. Biochem. Mol. Biol. 34, 517-525.
  4. Doucet. S. P., Squinto, S. P. and Bazan, N. G. (1990) Fos-Jun and the primary genomic response in the nervous system: possible physiological role and pathophysiological significance; in Molecular Neurobiology, Bazan, N. G. (ed.), pp. 27-55, Human, Clifton, New Jersey.
  5. Guo, H. F., Cui, X., Hou, Y., Tian, J., Wang, X. and Han, J. (1996) C-Fos proteins are not involved in the activation of preproenkephalin gene expression in rat brain by peripheral electric stimulation (electroacupuncture). Neurosci. Lett. 207, 163-166. https://doi.org/10.1016/0304-3940(96)12523-4
  6. Han, J. S. and Terenius, L. (1982) Neurochemical basis of acupuncture analgesia. Annu. Rev. Pharmacol. Toxicol. 22, 193-220. https://doi.org/10.1146/annurev.pa.22.040182.001205
  7. Hansson, P. (2002) Neuropathic pain: clinical characteristics and diagnostic workup. Eur. J. Pain 6, 47-50. https://doi.org/10.1053/eujp.2001.0322
  8. He, L. F. (1987) Involvement of endogenous opioid peptides in acupuncture analgesia. Pain 31, 99-121. https://doi.org/10.1016/0304-3959(87)90011-X
  9. Ho, W K. and Wen, H. L. (1989) Opioid-like activity in the cerebrospinal fluid of pain patients treated by electroacupuncture. Neurophannacol. 28, 961-966. https://doi.org/10.1016/0028-3908(89)90196-2
  10. Hwang, B. G., Min, B. I., Kim, J. H., Na, H. S. and Park, D. S. (2002) Effects of electroacupuncture on the mechanical allodynia in the rat model of neuropathic pain. Neurosci. Left. 320, 49-52. https://doi.org/10.1016/S0304-3940(02)00027-7
  11. Ji, R. R., Zhang, Z. W, Zhou, Y., Zhang, Q. and Han, J. S. (1993) Induction of c-fos expression in the rostral medulla of rats following electoacupuncture stimulation. Int. J. Neurosci. 72, 183-191. https://doi.org/10.3109/00207459309024107
  12. Kim, H.-H., Kim, H.-M., Kwack, K. B., Kim, S. W and Lee, Z. H. (2001) Osteoclast differentiation factor engages the PI 3- kinase, p38, and ERK pathways for avian osteoclast differentiation. J. Biochem. Mol. Biol. 34, 421-427.
  13. Kim, H. J., Na, H. S., Back, S. K. and Hong, S. K. (2001) Sympathetic sprouting in sensory ganglia depends on the number of injured neurons. Neuroreport 12, 3529-3532. https://doi.org/10.1097/00001756-200111160-00031
  14. Kimura, A and Sato, A (1997) Somatic regulation of autonomic functions in anesthetized animals-neural mechanisms of physical therapy including acupuncture. Jpn. J. Vet. Res. 45, 137-145.
  15. Lee, J. -H. and Beitz, A J. (1992) Electroacupuncture modifies the expression of c-fos in the spinal cord induced by noxious stimulation. Brain Res. 577, 80-91. https://doi.org/10.1016/0006-8993(92)90540-P
  16. Na, H. S., Han, J. S., Ko, K. H. and Hong, S. K. (1994) A behavioral model for peripheral neuropathy produced in a rat's tail by inferior caudal trunk injury. Neurosci. Lett. 177, 50-52. https://doi.org/10.1016/0304-3940(94)90042-6
  17. Pan, B., Castro-Lopes, J. M. and Coimbra, A. (1994) C-fos expression in the hypothalamo-pituitary systems induced by electroacupuncture or noxious stimulation. Neuroreport 5, 1649-1652 https://doi.org/10.1097/00001756-199408150-00027
  18. Talmoush, A. J. (1981) Causalgia: redefinition as a clinical pain syndrome. Pain 10. 187-197. https://doi.org/10.1016/0304-3959(81)90194-9

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