BMB Reports

생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
권호 표지
DOI QR코드

DOI QR Code

DNA Fingerprinting of Jute Germplasm by RAPD

  • Hossain, Mohammad Belayat (Department of Biochemistry & Molecular Biology, University of Dhaka) ;
  • Haque, Samiul (Physiology Department, Bangladesh Jute Research Institute) ;
  • Khan, Haseena (Department of Biochemistry & Molecular Biology, University of Dhaka)
  • 발행 : 2002.07.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The genotype characteristic of cultivars was investigated, along with varieties of both of the jute species, Corchorus olitorius and Corchorus capsularis, in the germplasm collection at the Bangladesh Jute Research Institute (BJRI). DNA fingerprinting was generated for 9 different varieties and 12 accessions of jute cultivars by using random amplified polymorphic DNA(RAPD). A total of 29 arbitrary oligonucleotide primers were screened. Seven primers gave polymorphism within the varieties, and 6 primers detected polymorphism within the accessions that were tested. A dendrogram was engendered from these data, and this gave a distinct clustering of the cultivated species of jute. Therefore, we generated RAPD markers, which are species-specific. These primers can distinguish between C. olitorius and C. capsularis. From the dendrogram that we generated between the various members of these two species, we found the existing genetic classification that agrees with our molecular marking data. A different dendrogram showed that jute accessions could be clustered into three groups. These data will be invaluable in the conservation and utilization of the genetic pool in the germplasm collection.

키워드

참고문헌

  1. Cheng, Z., Baldwin, B.S., Ohtani, Y. and Sameshima, K. (2000) Identification of Method and Genetic Relationship among Kenaf (Hibiscus cannobinus L.) Varieties based on RAPD (Random Amplified Polymorphic DNA) Analysis. Proceedings of the Final workshop on "Application of Biotechnology in the Improvement of .Jute, Kenaf and Allied Fibres- Phase II," (IJO/AGR/10) Beijing. China 10-12 August 2000. pp. 61-72.
  2. Debener, T., Bartels, C. and Matiesch, L. (1996) RAPD analysis of genetic variation between a group of rose varieties and selected wild rose species. Mol. Breeding 2, 321-327. https://doi.org/10.1007/BF00437910
  3. Doyle, J. J. and Doyle, J. L. (1990) Isolation of plant DNA from fresh tissue. Focus 12, 13-15.
  4. Edmonds, J. M. (1990) Herbarium Survey of African Corchorus L. Species, International Board For Plant Genetic Resources (IBPGR)/IJO. Rome, Italy pp. 32-33.
  5. Fujishiro, T. and Sasakuma, T. (1994) Variety identification and molecular characterization of newly bred line by RAPD marker in Brassica juncea. Breeding Science (Suppl.) 1, 132.
  6. Hanboonsong, Y., Vinijsanun, T. and Ponragdee, W. (2000) Molecular Characterization and Genetic Relationships of Roselle Germplasm in Thailand. Proceedings of the Final Workshop on "Application of Biotechnology in the Improvement of .lute, Kenaf and Allied Fibres-Phase II," (IJO/ AGR/10). Beijing, China 10-12 August 2000. pp. 95-106.
  7. Howell, E. C., Newbury, H. J., Swennen, R. L., Withers, L. A. and Ford-Lloyd, B. V. (1994) The use of RAPD for identifying and classing Musa germplasm. Genome 37, 328-332. https://doi.org/10.1139/g94-045
  8. Hu, J. and Quiros, C. F. (1991) Identification of broccoli and cauliflower cultivars with RAPD markers. Plant Cell Rep. 10, 505-511.
  9. Islam, A. S., Haque, M. M., Haque, M. I. and Seraj, Z. I. (1992) Tissue culture and micropropagation of jute (Corchorus spp.); in Biotechnology in Agriculture and Foresty, Vol 19, High Tech and Micropropagation, Bajaj, P. S. (ed.), pp. 505-526, Springer Veriag, Heidelberg. https://doi.org/10.1007/978-3-662-07770-2_31
  10. Kaemmer, D., Afza, R., Weising, K., Kahl, G. and Novak, F. J. (1992) Oligonucleotide and amplification fingerprinting of wild species and cultivars of banana (Musa spp.) Biotechnology 10, 1030-1035. https://doi.org/10.1038/nbt0992-1030
  11. Koller, B., Lehman. A., McDermott, J. M. and Gesseler, C. (1993) Identification of apple cultivars. Theor. Appl. Genet. 85, 901-904.
  12. Kresovich, S., Williams, J. G. K., McFerson, J. R., Routman, E. J. and Schaal, B. A. (1992) Characterization of genetic identities and relationships of Brassica oleracea L. via a random amplified polymorphic DNA assay. Theor. Appl. Genet. 85, 190-196.
  13. Lawson, W. R., Henry. R. J., Kochrnan,J1. K. and Kong, G. A. (1994) Genetic diversity in sunflower (Helianthus annuus L.) as revealed by random amplified polymorphic DNA analysis. Aust . J. Agric. Res. 45, 1319-1327. https://doi.org/10.1071/AR9941319
  14. Lin, J. J., Kuo, J., Ma, J., Saunders, J. A., Bread, H. S., Macdonald, M. H., Kenworthy, W., Ude, G. N. and Mattews, B. F. (1996) Identification of molecular markers in soybean comparing RFLP, RAPD and AFLP DNA Mapping Techniques. Plant Mol. Biol. Reporter 14, 156-169. https://doi.org/10.1007/BF02684905
  15. Novy, R. G., Kobak, C., Goffreda and Vorsa, N. (1994) RAPDs identify varietal misclassification and regional divergence in cranberry (Vaccinium macrocarpon (Ait.) Pursh). Theor. Appl. Genet. 88, 1004-1010.
  16. Powell, W., Morgante, M., Andre, C., Hanafey, M., Yogel, J., Tingey, S. V., Tingey, S. and Rafalski, A. (1996) The comparison of RFLP, RAPD, AFLP and SSR (microsattelite) markers for germplasm analysis. Mol. Breeding 2, 225-238. https://doi.org/10.1007/BF00564200
  17. StatSoft. (1994) STATISTICA Users Guide Version 4.1. Stat Soft Inc., Tulsa, USA 1064 pp.
  18. Stiles, J. I., Lemme, C., Sondur, S., Morshidi, M. B. and Manshardt, R. (1993) Using randomly amplified polymorphic DNA for evaluating genetic relationships among papaya cultivars. Theor. Appl. Genet. 85, 697-701.
  19. Takeuchi, A. (1994) Identification of close related varieties in Niigata Pref. Based on DNA markers (in Japanese). Breeding Science 44 (Suppl. 1), 129.
  20. Wilde, J., Waugh, R. and Powell, W. (1992) Genetic fingerprinting of Theobroma clones using randomly amplified polymorphic DNA markers. Theor. Appl. Genet. 83, 871-877.
  21. Wilkie, S. E., Issac, P. G. and Slater, R. J. (1993) Random amplified polymorphic DNA (RAPD) markers for genetic analysis in Allium. Theor. Appl. Genet. 86, 497-504. https://doi.org/10.1007/BF00838566
  22. Williams, J. G. K., Kubelik, A. K., Livac, K. J., Rafalski, J. A. and Tingey, S. V. (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18,6531-6535. https://doi.org/10.1093/nar/18.22.6531
  23. Yang, X. and Quiros, C. F. (1993) Identification and classification of celery cultivars with RAPD markers. Theor. Appl. Genet. 86, 205-212.
  24. Yazaki, S., Kawata, M., Monma, B., Muraki, M., Miura, Y., Takaiwa, F., Shimamto, Y. and Ueda, S. (1994) Analysis of DNA polymorphisms among maize inbred lines by RAPDs. Breeding Sci. 44 (Suppl. 1), 130.
  25. Zhou, Z., Bebeli, P. J., Somers, D. J. and Gustafson, J. P. (1998) Analysis of DNA polymorphisms among Kenaf (Hibiscus cannabinus L.) varieties by RAPD (Random Amplified Polymorphic DNA). Mol. Breeding 8, 232-347.

피인용 문헌

  1. Development of linkage map in F2 population of selected parents with respect toMacrophomina phaseolinaresistance trait using screened polymorphic RAPD and developed SCAR markers of jute vol.44, pp.7, 2011, https://doi.org/10.1080/03235400903308883
  2. SSR and RAPID Profile Based Grouping of Selected Jute Germplasm with Respect to Fibre Fineness Trait vol.17, pp.1, 2008, https://doi.org/10.1007/BF03263256
  3. Genetic assessment of eight Corchorus spp. (Tiliaceae) using RAPD and ISSR markers vol.56, pp.1, 2013, https://doi.org/10.1007/s13237-013-0076-6
  4. Origins of white (Corchorus capsularis L.) and dark (C. olitorius L.) jute: a reevaluation based on nuclear and chloroplast microsatellites vol.22, pp.4, 2013, https://doi.org/10.1007/s13562-012-0165-7
  5. A complete genetic linkage map and QTL analyses for bast fibre quality traits, yield and yield components in jute (Corchorus olitorius L.) vol.47, pp.3, 2013, https://doi.org/10.3103/S0095452713030092
  6. Genetic linkage map construction for white jute (Corchorus capsularisL.) using SRAP, ISSR and RAPD markers vol.133, pp.6, 2014, https://doi.org/10.1111/pbr.12205
  7. Evaluation of genetic diversity in jute (Corchorus species) using STMS, ISSR and RAPD markers vol.125, pp.3, 2006, https://doi.org/10.1111/j.1439-0523.2006.01208.x
  8. Collection and ethnobotanical investigation of Corchorus species in Ethiopia: potential leafy vegetables for dry regions vol.57, pp.2, 2010, https://doi.org/10.1007/s10722-009-9470-y
  9. A preliminary genetic analysis of fibre traits and the use of new genomic SSRs for genetic diversity in jute vol.161, pp.3, 2008, https://doi.org/10.1007/s10681-007-9597-x
  10. Distinction between Cold-sensitive and -tolerant Jute by DNA Polymorphisms vol.36, pp.5, 2003, https://doi.org/10.5483/BMBRep.2003.36.5.427