한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제31권1호
  • /
  • Pages.26-31
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  • 2002
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  • 1226-3311(pISSN)
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  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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Headspace법에 의한 솔향의 분석

Comparisons of Volatile Compounds Extracted from Pinus densiflora by Headspace Analysis

  • 이미정 (부경대학교 식품생명공학부·수산식품연구소) ;
  • 정은주 (부경대학교 식품생명공학부·수산식품연구소) ;
  • 이신조 (부경대학교 식품생명공학부·수산식품연구소) ;
  • 조지은 (부경대학교 식품생명공학부·수산식품연구소) ;
  • 이양봉 (부경대학교 식품생명공학부·수산식품연구소) ;
  • 조현종 (농협중앙회 식품연구소) ;
  • 윤정로 (강릉대학교 식품과학과)
  • 발행 : 2002.02.01
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초록

Pinus densiflora(2엽송) 1년생을 채취하여 가지, 잎, 새순 부분으로 구분하고, 시료병에 넣어 밀봉한 뒤, solide phase microextraction (SPME)과 dynamic headspace analysis(DHA)를 이용하여 휘발성 성분을 분리, 동정하였다. SPME와 DHA의 결과를 terpenes의 구성 성분에 따라 monoterpenes과 sesquiterpenes, oxygenated terpenes으로 나누어 조성비를 살펴보면, monoterpenes의 조성은 잎의 경우 각각 66.7%와 14.3%, 새순은 90.6%와 0.7%, 가지에서는 90.6%와 1.2%로 나타났다. Sesquiterpenes의 함량은 SPMe의 결과에서만, 잎이 25.8%, 가지 4.4%, 새순 1.5%로 나타났다. 산소를 함유하고 있는 terpenes의 함량은 새순 부위가 4.7%, 79.0%로 나타났으며, 가지 부위는 3.7%와 70.4%, 잎에서 1.0%와 50.7%를 각각 차지하였다. SPME의 결과는 이전의 용매추출을 통한 결과와 다소 비슷한 양상을 보였으며, DHA의 결과는 10$0^{\circ}C$이상의 고비점 화합물들이 나타나지 않은 반면, 5$0^{\circ}C$이하의 저비점 화합물이 15개 분리되었다.

Volatile compounds from three parts of needles, sprouts and twigs in Pinus densiflora were extracted by solid phase microextraction (SPME) and dynamic headspace analysis (DHA). separated by gas chromatography, and identified by using mass selective detecter and Kovat's retention index. The amounts of monoterpenes isolated by SPME and DHA were 66.7% and 14.3% in needles, 90.6% and 0.7% in sprouts, and 90.6% and 1.2% in twings, respectively. The amounts of sesquiterpenes detected only by SPME were 25.8% in needles, 4.4% in twings and 1.5% in sprouts. And the amounts of oxygenated terpenes isolated by SPME and DHA were 4.7% and 79.0% in sprouts, 3.7% and 70.4% in twigs, and 1.0% and 50.7% in needles, respectively. The kinds of volatile compounds isolated by SPME were similar to those by solvent extraction, but the number of compounds identified to hate a boiling point below 5$0^{\circ}C$ by UHA was larger than that of SPME.

키워드

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피인용 문헌

  1. Volatile components and antibacterial effects of pine needle (Pinus densiflora S. and Z.) extracts vol.22, pp.1, 2005, https://doi.org/10.1016/j.fm.2004.05.002
  2. Rapid Diagnosis of the Infection of Pine Tree with Pine Wood Nematode (Bursaphelenchus xylophilus) by Use of Host-Tree Volatiles vol.60, pp.30, 2012, https://doi.org/10.1021/jf302484m
  3. Immunosuppressive Effect of the Intraperitonially Injected Pine Needle Distillate in Mice vol.8, pp.1, 2002, https://doi.org/10.3746/jfn.2003.8.1.007