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Effect of Eucommia ulmoides Leaf Water Extract on Hepatotoxicity of Carbon Tetrachloride-Induced Rats

두충잎 물추출물이 사염화탄소를 투여한 흰쥐의 간손상에 미치는 영향

  • 전정례 (영남대학교 식품영양학과) ;
  • 박정륭 (영남대학교 식품영양학과)
  • Published : 2002.02.01

Abstract

The present study was carried out to investigate the pretreatment of effects of water extract obtained from Eucommia ulmoides leaf (ELE) on hepatotoxicity of carbon tetrachloride ($CCl_4$) -treated rats. Thirty two healthy male Sprague-Dawley rats were divided into four groups, i.e. normal group (N), $CCl_4$-treated group (T), ELE-treated group (E), and ELE-treated group after injection of $CCl_4$(TE). The administration of $CCl_4$increased the activities of aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase in serum, but their activities were significantly decreased by the addition of ELE. Alkaline phosphatase activity in serum was significantly decreased in $CCl_4$-treated group, while the activity was not decreased by the extracts. Higher levels of HDL cholesterol was found in E and TE group which showed lower levels of total and LDL-cholesterol. Histopathologic findings by light microscopic examination showed fatty change, ballooning degeneration, acidophilic degeneration, spotty necrosis and zonal necrosis in $CCl_4$treated liver tissue. But the degree of liver damage was not identified in liver tissue of the TE group. These results indicated that Eucommia ulmoides leaf water extracts led migtigation of liver demage induced with $CCl_4$.

두충잎의 생약 및 식품 자원으로서의 이용성을 증진시킬 목적으로 두충잎의 물추출물을 사염화탄소를 투여한 흰쥐의 간손상에 미치는 영향을 검토하기 위해, 정상군, 사염화탄소군, 두충잎 물추출액 투여군, 사염화탄소 처리 후 두충잎 물추출액을 투여한 군의 4개군으로 나누어 6주간 사육하여 얻은 결과는 다음과 같다. 혈청 중의 AST, ALT, LDH 활성은 사염화탄소 투여에 의해 현저히 증가하였으나 두충잎 물추출물을 함께 투여함으로 정상군과 수준으로 회복되었다. 한편 사염화탄소에 의해 증가된 혈청중의 ALP 활성은 두충잎 투여에 의해 유의성 있게 감소하지 않았다. 혈청 중의 중성지질, 총 콜레스테롤 함량과 LDL콜레스테롤의 함량은 사염화탄소군에서만 유의적인 증가를 나타내었을 뿐 나머지 각군간의 유의적인 차이는 없었다. 한편 혈청 중의 HDL콜레스테롤의 함량은 두충 단독 투여군 및 사염화탄소 투여 후 두충잎 투여군이 정상군 및 사염화탄소 단독 투여군들보다 유의적으로 높게 나타났다. 간조직 중의 총콜레스테롤, 총지질, 중성 지질의 함량은 사염화탄소에 의해 유의적인 증가를 나타내었으나 두충잎 물추출액의 투여로 인해 이들의 함량이 유의성 있는 감소는 관찰되지 않았다. 광학현미경에 의한 간소직 세포의 병리학적인 검사에서는 사염화탄소군에서 간손상시 나타나는 간조직의 지방변화, 풍선양 변성, 호산성 변성, 국소성 괴사, 부위별 괴사가 관찰되었으나 그후 두충잎 투여에서 변화가 나타나지 않아 간 손상이 회복되었음을 알 수 있었다. 이상의 결과에서 두충잎 열수 추출물은 $CCl_4$에 의해 손상된 간기능을 회복시키는데 효과가 있었으며, 특히 혈청지질 개선에 탁월한 효과가 있음을 알 수 있었다.

Keywords

References

  1. 小學館: 中藥大辭典Ⅲ. 上海, 上海科學技術出版社, p.1031-1032 (1964)
  2. 喬木敬次郞, 木村正廉, 原田正敏, 大土家公男: 和漢藥物學. 東京, 南山堂, p.159 (1982)
  3. 中國衛生部葯典委員會: 中國藥典(中草及其製品). 上海, 人民衛生出版社, p.285 (1978)
  4. 簡東緖: 家免血壓. 山阜醫記, Vol. 5, p.480 (1957)
  5. Chung, M.H. and Park, C.W. : Studies on the development of antihypertensitive agents from Korean crude drug. Ⅰ,Ⅱ, Ⅲ, Kor. J. Pharmacol., 6, 29-42 (1975)
  6. Hong, N.D., Rho, Y.S., Won, D.H., Kim, N.J. and Cho, B. S. : Studies on the anti-diabetic activity of Eucommia ulmoides Oliver. Kor. J. Pharmacol., 18, 112-117 (1987)
  7. Hong, N.D., Rho, Y.S., Kim, J.W., Won, D.H., Kim, N.J. and Cho, B.S. : Studies on the general pharmacological activities of Eucommia ulmoides Oliver. Kor. J. Pharmacol., 19, 102-110 (1988)
  8. Lee, M.H. : Extraction and purification of anticancer components in Eucommia ulmoides leaves. M.S. Thesis, Korea University (1988)
  9. Park, J.C. and Kim, S.H. : Flavonoid analysis from the leaves of Eucommia ulmoides. J. Korean Soc. Food Sci. Nutr.,24, 901-905 (1995)
  10. Kim, J.B. : Changes of major components during growth and physiological functionality of Eucommia ulmoides leaves. Ph.D. Thesis, Yeungnam University (1999)
  11. Anita, K.C. and James, R.T. : Interaction of hypoxia and carbon tetrachloride toxicity in hepatocyte monolayer. Experimental and Molecular Pathology, 50, 183-189 (1989) https://doi.org/10.1016/0014-4800(89)90029-4
  12. Amdur, M.O., Doull, J. and Klaassen, C.D. : Toxicology. 4th ed., Pergamon Press, p.338 (1991)
  13. Reitman, S. and Frankel, S. : A colorimetric method for determination of serum glutamic oxalacetic and glutamic pyruvic transaminase. Am. J. Clin. Path., 28, 58-63 (1954)
  14. Karmen, A. : A note on the spectrometric assay of glutamicoxalacetic transaminase in human blood serum. J. Clinic. Invst., 34, 131-139 (1955)
  15. Berga, L. and Broida, D. : Sigma Tech. Bull. Sigma Co., Vol. 500, No. 8, p.60-61 (1960)
  16. Kind, R.N. and King, E.T. : Estimation of plasma phophatase by determination of hydrolyzed with antipyrene. J. Clinic. Pathol., 7, 332-338 (1954)
  17. Tietz, N.W. : Fundamentals of Clinical Chemistry. 2nd ed., W. B. Saunder, Philadelphia, p.606-609 (1959)
  18. Frings, C.S. and Dunn, R.T. : A colorimetric method for determination of total serum lipid based on the sulfophosphovanillin reaction. Am. J. Clin. Path., 53, 89-91 (1970) https://doi.org/10.1093/ajcp/53.1.89
  19. Muller, P.H. : A fully enzymatic triglyceride determination. J. Clin. Chem. Clin. Biochem., 15, 457-464 (1977)
  20. Richmond, W. : Use of cholesterol oxidase for assay of total and free cholesterol in serum by continuous flow analysis. Clin. Chem., 22, 1579-1588 (1976)
  21. Folch, J., Lee, M. and Stanley, G.H. : A simple method for the isolation and purification of total lipid from animal tissues. J. Biol. Chem., 226, 497-502 (1975)
  22. Zak, B. and Dickman, R.C. : Rapid estimation of free and total cholesterol. Am. J. Clin. Pathol., 24, 1307-1314 (1954) https://doi.org/10.1093/ajcp/24.11_ts.1307
  23. Laemmli, U.K. : Cleavage of the structural protein during the assembly of the head of bacteriophage T4. Nature, 227,680-685 (1970) https://doi.org/10.1038/227680a0
  24. Lee, K.H., Park, H.C. and Her, E.S. : Statistics and data analysis method. Hyoil Press, Seoul, p.253-296 (1998)
  25. Bae, S.J., Kim, N.H., Ha, B.J., Jung, B.M. and Roh, S.B. : Effect of Godulbaegi leaf extracts on CCl4-induced hepatotoxicity in rats. J. Korean Soc. Food Sci. Nutr., 26, 137-143 (1997)
  26. Mun, S.I., Ryu, H.S. and Choi, J.S. : Inhibition effects of Zanthoxylum schinifolium and its active principle on lipid peroxidation and liver damage in carbon tetrachloride treated mice. J. Korean Soc. Food Sci. Nutr., 26, 943-951 (1997)
  27. Yoon, C.G., Lee, S.I. and Shin, J.K. : Effect of carbon tetrachloride on the changes of xanthine oxidase activity in rats previously fed low or high protein diet. J. Korean Soc. Food Sci. Nutr., 20, 527-537 (1991)
  28. Lee, M.Y. and Jun, S.J. : Effect of Cuscutae extract on experimentally induced liver damage in rabbits. J. Korean Soc. Food Sci. Nutr., 18, 333-337 (1989)
  29. Chung, M.H., Kang, S.C. and Kim, K.W. : The acute toxicity of Liocolae vermiculus extract in mice and its effect on hepatic damage induced by CCl4 in rats. Kor. J. Pharmacogn., 22, 36-44 (1991)
  30. Han, E.G. : Effect of Codonopsis lanceolata extracts on carbontetrachloride-induced hepatotoxicity. Ph.D. Thesis, Yeungnam University (1996)
  31. Kim, G.H. and Han, H.K. : The effect of mushroom extracts on carbon tetrachroride-induced hepatotoxicity in rats. J.Korean Soc. Food Sci. Nutr., 27, 326-332 (1998)
  32. Grundy, S.M. : Monounsaturated fatty acids, plasma cholesterol and coronary heart disease. Am. J. Clin. Nutr., 45, 1168-1174 (1987) https://doi.org/10.1093/ajcn/45.5.1168
  33. Joo, E.Y. : Induction of cytochrome p450 protein in wounded avocado roots (Persea americanna, Mill). J. Korean Soc.Food Sci. Nutr., 2, 328-332 (1997)
  34. Bae, J.T. : Studies on the cancer chemoprevention of Sarcodon aspratus extracts. Ph.D. Thesis, Yeungnam University (1999)

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