Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Enhancement of Anticarcinogenic Effect by Combination of Sedum sarmentosum Bunge with Platycodon grandiflorum A. Extracts

도라지 추출물 첨가에 의한 돌나물의 항발암 상승효과

  • 박윤자 (신라대학교 식품영양학과) ;
  • 김미향 (신라대학교 식품영양학과) ;
  • 배송자 (신라대학교 식품영양학과)
  • Published : 2002.02.01
Download PDF
⟨ Previous Next ⟩

Abstract

Anticarcinogen is one of the major strategies for cancer control. It is well established that dietary factors play an important role in modulating the development of certain types of human cancer. We investiagted the anticarcinogenic effects of Sedum sarmentosum Bunge (SS) with Platycodon grandiflorum A. extracts on HepG2, HeLa and MCF-7 cell lines. By the MTT assay, among the five partition layers of methanol extract of SS (SSM), the ethylether partition layer of SS (SSMEE) showed the strongest cytotoxic effects on all cell lines. We also investigated the synergistic effect of the combination of SS and PG extracts on growth inhibition of the HepG2, HeLa and MCF-7 cell lines compared to the effects of five partition layers of SSM. Combination of SS and PG extracts significantly increased cytotoxic effects on all cell lines. Therefore, we were able to conclude that ethylether partition layer, SSMEE might have potentially useful cytotoxic materials on all the human cancer cells which we used. And we could suggest that the combination of SS with PG enhanced the anticarcinogenic effect on HepG2, HeLa and MCF-7 cell lines. We also determined QR activity of partition layers of SSM, among them, SSMEE on HepG2 cells showed the highest QR activity, 3.21 as control value of 1.0.

본 연구는 다른 산채류에 비해 칼슘이 칼슘이 특히 많은 우수한 식품으로 예부터 물김치나 겉절이 무침, 돌나무 김치로 많이 이용되어온 돌나물의 암세포 증식억제 및 암예방 효과를 검색하고 나아가 도라지 추출물과의 암세포 증식억제 상승효과를 보기 위하여 실시하였다. 돌나물의 암세포 증식억제 효과를 MTT assay로 실험한 결과, 3종의 암세포주(HepG2, HeLa, MCF-7) 모두 돌나물의 ethylether 분획층(SSMEE)에서 아주 높은 암세포 증식억제 효과를 보였으며, ethylacetate 분획층(SSMEA)에서도 유의적인 암세포 증식억제 효과를 나타내었다. 또한, 돌나물 분획물의 암예방 QR유도 활성을 HepG2 세포주를 이용하여 실험한 결과, 다른 분획층에 비해 비극성 용매층인 ethylether 분획층(SSMEE)과 hexane 분획층(SSMH)에서 유의적으로 QR유도 활성을 증가시키는 것으로 나타났다. 한편, 돌나물과 도라지와의 일정비 조합을 통해 상승효과를 본 결과, $IC_{50}$/이 되는 도라지의 butanol층 (PGMB)dfm 일정량 첨가했을 때 용매별 돌나물의 분획층 모두에서 암세포 증식억제를 상승시키는 효과를 나타내었고, 암예방 QR 유도활성 효과도 훨씬 상승하였으므로, 돌나물 조리시 유사한 부재료를 사용하는 도라지의 성분을 일정량 첨가하여 겉절이 무침이나 김치로 이용될 때 돌나물 성분의 생리활성 효과가 더욱 상승될 것으로 사료되며, 이후 돌나물의 생리활성 물질 구조 및 기전 규명에 유익한 자료가 될 것으로 사료된다.

Keywords

References

  1. Weinstein, I.B. : Cancer prevention : Recent progress and future opportunities. Cancer Res., 51, 5080s-5085s (1991)
  2. Lee, K.Y., Lee,Y.C., Park, Y.S., Yoon, K.H. and Kim, B.S.: A study of relation between dietary vitamin A intake and serum vitamin A levels and cancer risk in Korea. Korean J. Nutr., 18, 301-311 (1995)
  3. Cooper, G.H. : Elements of Human Cancer. Jones and Bantlett publishers, Inc., Boston, p.31 (1992)
  4. Doll, R. and Peto, R. : The causes of cancer quantitative estimate of avoidable risks of cancer in the united states today. J. Natl. Cancer Inst., 66, 1191-1197 (1981)
  5. Leu, T.H. : Reaction of food, nutrition and cancer. J. Korean Food Sci. Nutr., 14, 305-311 (1985)
  6. Wakabayashi, K., Nagao, M., Esumi, H. and Sugimura, T. : Food derived mutagens and carcinogens. Cancer Res. (suppl), 52, 2092-2098 (1992)
  7. Dewys, W.D., Malone, W.F., Butrum, R. and Setili, M.A.: Clinical trials in cancer prevention cancer. Cancer Res., 60, 650-656 (1987)
  8. Murakami, A., Ohigashi, H. and Soshimizu, K. : Anti tumor promotion with food phytochemicals : A stategy for cancer chemoprevention. Biosci. Biotech. Biochem., 60, 1-9 (1996) https://doi.org/10.1271/bbb.60.1
  9. Calls, L.M. and Sullivan, P.D. : Screening of antioxidants and other compounds for antimutagenic properties towards benzo(a)pyrene-induced mutagenicity in strain Ta98 of salmonella typhimurium. Mutat. Res., 101, 99-105 (1982) https://doi.org/10.1016/0165-1218(82)90001-5
  10. Hasler, C.M. : Functional foods : Their role in disease prevention and health promotion. Food Technol., 52, 63-70 (1998)
  11. Messina, M. and Barnes, S. : The role of soy products in reducing risk of cancer. J. Natl. Cancer Inst., 83, 541-546 (1991) https://doi.org/10.1093/jnci/83.8.541
  12. Clinton, S.K. : Lycopene: Chemistry, biology and implications for human health and disease. Nutr. Rev., 56, 35-51 (1998) https://doi.org/10.1111/j.1753-4887.1998.tb01691.x
  13. Gould, M.N. : Cancer chemoprevention and therapy by monoterpenes. Environ. Health Perspec., 105, 977-979 (1997) https://doi.org/10.2307/3433313
  14. Katiyar, S.K. and Mukhtar, H. : Tea in chemoprevention of cancer : Epidemiologic and experimental studies (review). Intl. J. Oncol., 8, 221-238 (1996)
  15. Han, E.J., Roh, S.B. and Bae, S.J. : Effects of quinone reductase induction and cytotoxicity of the Angelica radix extracts. J. Korean Soc. Food Sci. Nutr., 29, 147-152 (2000)
  16. Shim, S.M., Choi, S.W. and Bae, S.J. : Effects of Punica grantum L. Fractions on quinone reductase induction and growth inhibition on several cancer cells. J. Korean Soc.Food Sci. Nutr., 30, 80-85 (2001)
  17. Choi, S.Y., Cheigh, M.J., Lee, J.J., Kim, H.J., Hong, S.S., Chung, K.S. and Lee, B.K. : Growth suppression effect of traditional fermented soybean paste (doenjang) on the various tumor cells. J. Korean Soc. Food Sci. Nutr., 28, 458-463 (1999)
  18. Cheng, G.C., Lee, J.Y., Kim, D.C., Suh, S.O. and Hwang, W.I. : Inhibitory effect of Salvia miltiorrhiza extract on growth of some cancer cells. J. Korean Soc. Food Sci. Nutr., 29, 726-731 (2000)
  19. Kim, H.J., Han, J., Yang, E.J., Lee, K.R. and Lee, I.S. : hemoprevention effect of Polyozellus nultiplex, a wild and edible mushroom. J. Korean Soc. Food Sci. Nutr., 29, 161-167 (2000)
  20. Choi, O.J. : Component and using of medical plants. Ilwoulsegak, Seoul, p.312-313 (1999)
  21. Michael, C.A., Dominic, A.S., Anne, M., Miriam, L.H., Maciej, J.C., Donald, L.F., Betty, J.A., Joseph, G.M., Robert, H.S. and Michael, R.B. : Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Res., 48, 589-601 (1988)
  22. Carmichael, J., De Graff, W.G., Gazder, A.F., Minna, J.D. and Mitchell, J.B. : Evalution of a tetrazolium based semiautomated colorimetric assay : assessment of chemosensitivity testing. Cancer Res., 47, 936-942 (1987)
  23. Prochaska, H.J. and Santamaria, A.B. : Direct measurement of NAD(P)H : Quinone reductase from cells cultured in microtiter wells : A screening assay for anticarcinogenic enzyme inducers. Anal. Biochem., 169, 328-336 (1988) https://doi.org/10.1016/0003-2697(88)90292-8
  24. Park, H.J. : Induction of quinone reductase and its regulatory mechanism at the transcriptional level by Scutellaria baicalensis. Ph.D. Dissertation, Yonsei University (1998)
  25. Benson, A., Hunkeler, M.J. and Talaly, P. : Increase of NAD (P)H : quinone reductase by dietary antioxidants; possible role in pretection against carcinogenesis and toxicity. Proc. Natl. Acad. USA, 77, 5216-5220 (1980) https://doi.org/10.1073/pnas.77.9.5216
  26. Prostera, T., Holtzclaw, W.D., Zhang, Y. and Talaly, P. : Chemical and detoxify carcinogens. Proc. Natl. Sci. USA, 90, 2965-2969 (1993) https://doi.org/10.1073/pnas.90.7.2965
  27. Kang, H.J. : Screening for anticarcinogenic enzyme inducer(s) from roasted and defatted perilla. M.S. Thesis, Pusan National University (1998)

Cited by

  1. Plant regeneration and soil acclimatization through photoautotrophic culture from leaf explant of a rare species in Sedum tosaense Makino vol.40, pp.2, 2013, https://doi.org/10.5010/JPB.2013.40.2.079
  2. A Study on Microbiological Hazards in Sterilization Processing of Pteridium aquilinum and Platycodon grandiflorum vol.17, pp.1, 2016, https://doi.org/10.5762/KAIS.2016.17.1.646
  3. 돌나물의 기내 활성보존에 영향하는 요인 vol.22, pp.3, 2002, https://doi.org/10.12791/ksbec.2013.22.3.241
  4. 도라지 분말 첨가량에 따른 생면의 품질 특성 vol.33, pp.1, 2002, https://doi.org/10.9799/ksfan.2020.33.1.037