Optimal Condition for Mycelial Growth of Beauveria bassiana and Its Extracellular Enzyme Activity

백강균(Beauveria bassiana)의 균사체 최적 배양조건 및 효소활성

  • Published : 2002.03.01

Abstract

The optimum temperature and pH for mycelial growth of B. bassiana DGUM 34001 were $24^{\circ}C$ and pH 7.0, respectively. Among the complex media used, mushroom complex medium (MCM) was the most favorable for mycelial growth. When Czapek-Dox medium was used as a minimal medium, glucose was an excellent source for carbon and energy. Soytone and sodium phosphate were favorable constituent for culture medium as a source of organic nitrogen and phosphorus, respectively. When the fungus was grown in MCM broth, the specific activity of extracellular enzyme of ${\alpha}$-amylase, lipase, chitinase, CMCase and pretease were 297.0, 0.058, 0.33, 0.21 and 22.8 units/mg protein, respectively. When various sources of organic nitrogen and chitin were supplemented to determine the production of enzymes, casein and soluble chitosan enhanced the production of extracellular protease and chitinase.

백강균(Beauveria bassiana DGUM 34001)은 $24^{\circ}C$의 온도와pH 7.0의 초기 pH에서 최적의 균사체 생육을나타내었다. 사용한 복합배지 중 mushroom complex배지(MCM)에서 가장 우수한 균사생육을 나타내었다. Czapek-Dox 한천배지를 최소배지로 각각 탄소원, 질소원 및 인산원의 영향을 조사한 결과, glucose, soytone 및 sodium phosphate ($NaH_2$$PO_4$)에서 가장 우수한 균사 생육을 보여주었다. MCM액체배지에서 균사 배양 후 세포외 효소 활성을 측정한 결과,$\alpha$-amylase, lipase, chitinase, CMCase및 pretense의 비효소활성은 각각 297.0, 0.058, 0.33, 0.21 및 22.8 units/mg protein이었다. Casein과 soluble chitosan을 첨가할 경우 세포외 분비 pretense와 chitinase의 효소 활성 이 증가되었다.

Keywords

References

  1. 한국균학회지 곤충병원성 진균 Beauveria속의 동정 및 계통분석에 관한 연구 김기영;이재동;민응기;한영환
  2. 미생물학회지 v.36 녹강균(Metarhizium anisopliae)의 최적 배양조건 및 효소활성 박영일;한영환
  3. 한국약용버섯도감 박완희;이호득
  4. 한국균학회지 v.26 송이균(Tricholoma matsutake) 배양액의 세포외 효소 활성 이창윤;홍운표;정명준;한영환
  5. Can. J. Bot. v.73 Comparative analysis of the in vivo and in vitro metabolites produced by the entomopathogen Beauveria bassiana Boucias, D.G.;I. Mazet;J. Pendland;S.Y. Hung https://doi.org/10.1139/b95-363
  6. Appl. Env. Microbiol. v.53 Purification and properties of an extracellular protease produced by the entomopathogenic fungus Beauveria bassiana Bidochka, M.J.;G.G. Khachatourians
  7. J. Inverteb. Pathol. v.41 Growth and sporulation of Beauveria bassiana and Metarhizium anisopliae in a basal medium containing various carbohydrate sources Campbell R.K.;G.L. Barnes;B.O. Cartweright;R.D. Elkenbary https://doi.org/10.1016/0022-2011(83)90242-2
  8. J. Gen. Microbiol. v.135 Ultrastructural localization of a cuticle-degrading protease produced by the entomophathogenic fungus Metarhizium anisopliae during penetration of host (Manduca sexta) cuticle Goettel, M.S.;R.J. Leger;N.W. Rizzo;R.C. Staple;D.W. Roverts
  9. Toxicol. v.35 Toxic properties of Beauveria bassiana pigments on erythrocyte membranes Jeffs, L.B.;G.K. George
  10. J. Inverter. Pathol. v.17 Toxins of the entomophagous fungus Beauveria bassiana Ⅱ. Effect of nitrogen sources ofn formation of the toxic protease in submerged culture Kucera, M. https://doi.org/10.1016/0022-2011(71)90093-0
  11. Indian J. Exp. Biol. v.30 Effect of temperature, pH and light on toxin production by Beauveria bassiana Sumita, S.;G.P. Agarwal;R.C. Rajak
  12. Proc. Biochem. v.34 Impact of process parameters on chitinase production by an alkalophilic marine Beauveria bassiana in solid state fermentation Suresh, P.V.;M. Chandrasekaran https://doi.org/10.1016/S0032-9592(98)00092-2