The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
권호 표지

Antibacterial Activities of Honeys on the Staphylococcus aureus

Staphylococcus aureus에 대한 벌꿀의 항균 활성

  • 백승화 (도립 충북과학대학 식품생명과학과) ;
  • 정동현 (한국양봉농업협동조합)
  • Published : 2002.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study examined for the investigation the effect of honey on antibacterial activity. The experimental honey were used the domestics, or chestnut honey, multiflower honey, acassia honey, native honey and the foreign, or manuka honey, clover honey, canola honey, and the artificial honey, made with the diluted solution of each 12.5%, 25.0%, 50.0%. The result of compared the occasion of added-catalase with not added-catalase about the honey's antibacterial activity on Staphylococcus aureus by agar well diffusion assay were as follows. When the catalase was not added, manuka honey antibacterial activity was superior to chestnut honey's in the diluted honey of 12.5% and on the occasion of the diluted honey of 25.0%, it was approved in the order of manuka honey > chestnut honey > multiflower honey 〉 native honey > clover honey > acassia honey and the occasion of the diluted honey of 50.0%, it was approved in the order of manuka honey > chestnut honey > canola honey > native honey > multiflower honey > clover honey > acassia honey(p > 0.01). The clear zone representing inhibition of growth in diluted honey of 12.5, 25.0, 50.0 % with non-treat catalase ranged from 5.85 to 6.60, 4.26 to 8.27, 5.24 to 11.49 mm, respectively. When the catalase was added, antibacterial activity only showed in the manuka honey of 12.5% and on the occasion of the diluted honey of 25.0%, manuka honey's antibacterial activity was superior to chestnut honey (p > 0.01). On the occasion of the diluted honey of 50.0%, antibacterial activity was high in the order of manuka honey > chestnut honey > clover honey > canola honey > native honey(p > 0.01). The correlation was approved significantly among the manuka honey, chestnut honey, clover honey, canola honey and native honey.

본 실험은 벌꿀이 항균활성에 미치는 영향을 규명하기 위해 국내산 벌꿀인 밤꿀, 잡화, 아카시아, 재래종 벌꿀과 외국산 벌꿀인 마누카, 클로버, 캐놀라 벌꿀 그리고 인공벌꿀을 각각 12.5%, 25.0%, 50%의 희석액으로 조제하여 catalase무첨가 또는 첨가한 경우에 있어서 벌꿀의 Staphylococcus aureus에 대한 항균활성을 agar well diffusion assay로 비교한 바 다음과 같은 결과를 얻었다. Catalase 무첨가의 경우 12.5%희석한 벌꿀은 마누카꿀 > 밤꿀이, 25.0%로 희석한 벌꿀은 마누카 꿀 > 밤꿀 > 잡화꿀 > 재래종꿀 > 클로버 꿀 > 아카시아꿀이, 50.0%로 희석한 벌꿀은 마누카꿀 > 밤꿀 > 캐롤라꿀 > 재래종꿀 > 잡화꿀>클로버꿀 > 아카시아꿀 순으로 항균활성이 인정되었다(p>0.01). Catalase 무첨가의 경우 12.5%, 25.0%, 50.0%로 희석한 벌꿀의 생육억제환은 각각 5.85∼6.60mm, 4.26∼8.27 mm, 5.24∼11.49mm 범위였다. Catalase 첨가의 경우 12.5%로 희석한 벌꿀은 마누카꿀에서 만 항균활성을 나타냈다. 25.0%로 희석한 벌꿀은 마누카꿀이 밤꿀보다 항균활성이 더 높게 나타냈다.(p > 0.01). 50.0%로 희석한 벌꿀은 마누카꿀 > 밤꿀>클로버꿀)캐롤라꿀>재래종꿀 순으로 항균활성이 높았으며 마누카꿀, 밤꿀, 클로버꿀, 캐롤라꿀, 재래종꿀 사이에서 유의성이 인정되었다(p > 0.01). Catalase 첨가의 경우 12. 5%, 25.0%, 50.0%로 희석한 벌꿀의 생육억제환은 각각 5.89mm, 5.01∼6.84mm, 3.10 ∼8.28mm범위였다.

Keywords

References

  1. Gunther, R. T. : (Reprinted) The Greek Herbal of Dioscorides, Hafner, New York
  2. Sackett, W. G. : Honey as a carrier of intestinal diseases. Bull. Colo. St. Univ. Agric. Exp. Stn., No. 252(1919)
  3. Dold, H., Du, D. H., and Dziao, S. T. : Nachweis antibakterieller, hitze-und lichtemp-findlicher Hemmungssoffe(inhibine) im Naturhonig Blutenhonig Z. Hyg. Infekt. Krankh., 120, 155-167(1937) https://doi.org/10.1007/BF02178015
  4. White, J. W., Mary, J. R., Subers, H. and Schepartz, A. I. : The identification of inhibine, the antibacterial factor in honey as hydrogen peroxide and its orgin in a honey glucose-oxidase system, Biochim. Biophys. Acta., 73, 57-70(1963) https://doi.org/10.1016/0006-3002(63)90359-7
  5. White. J. W. and Subers, M. H. : Studies of honey inhibine, 3. The effect of heat. J. Apic. Res., 3, 454-450(1964)
  6. Dustmann, J. H. : Ueber den Einfluss des Lichtes auf den peroxid-Wert des Honigs, Z. Lebensm., Unters. Forsch., 148, 263-268(1972) https://doi.org/10.1007/BF01458717
  7. Molan, P. C. : The Antimicrobial activity of honey. l. The nature of the antibacterial activity, Bee Wld., 73, 15~28(1992)
  8. Willix, D., Molan, P. C. and Reid, G. M. : A comparison of the sensitivity of wound infecting species of bacteria to the antibacterial activity of Manuka honey and other honey, J. Appl. Bacteriol., 73(5) 388-394(1992) https://doi.org/10.1111/j.1365-2672.1992.tb04993.x
  9. Molan, P. C. and Russel K. M. : Non-peroxide antibacterial activity in some New Zealand Honeys, J. Apic. Res., 27(1) 62-67(1988) https://doi.org/10.1080/00218839.1988.11100783
  10. Molan, P. C., Smith, I. M. and Reid, G. M. : A comparison of the antibacterial activity of some New Zealand Honeys. J. Apic. Res., 27(4) 252-256(1988) https://doi.org/10.1080/00218839.1988.11100811
  11. Russell, K. M. et al. : Identification of some Antibacterial constituents of New Zealand Manuka Honeys, J. Agric. Food Chem., 38, 10-13(1990) https://doi.org/10.1021/jf00091a002
  12. Allen, K. L., Molan, P. C. and Reid, G. M. : A Survey of the Antibacterial activity of some New Zealand Honeys, J. Pharm. Pharmacol., 43, 817-822(1991) https://doi.org/10.1111/j.2042-7158.1991.tb03186.x
  13. Molan, P. C. and Allen, K. L. : The effect of Gamma irradiation on the antibacterial activity of honey, J. Pharm. Pharmacol., 48, 1206-1209(1996) https://doi.org/10.1111/j.2042-7158.1996.tb03922.x
  14. Bogdanov, S. : Non-peroxide antibacterial activity of honey, Bee products, New York, 39-47(1996)
  15. Wahdan, H. A. : Causes of the antibacterial activity honey, Infection, 26(1) 26-30(1998) https://doi.org/10.1007/BF02768748
  16. Weston, R. J. et al. : Antibacterial phenolic components of New Zealand Manuka Honey, Food Chem., 65, 295-301(1999)
  17. Dustmann, J. H. : Antibacterial eggect honey. Apiacta. 14, 7-11(1979)
  18. Morse, R. A. : The antibiotic properties of honey. Panpacific Entomologist. 62, 370-371(1986)
  19. Hawer, W. D., Ha, J. H., and Nam, Y. J. : The Quality Assessment of Honey by Stable Carbon Isotope Analysis. Analytical Sci. & Techn., 5, 229-234(1992)
  20. Adcock, D. : The effect of catalase on the inhibine and peroxide values of various honeys. J. Apic. Res., 1, 38-40(1962) https://doi.org/10.1080/00218839.1962.11100047
  21. Echigo, T. : Honey, Honeybee Sci., 5(4) 167-176(1984)
  22. Effem, S. E. E. : Clinical observation on the wound healing properties of honey. Br., J. Surg., 75, 679-681(1988) https://doi.org/10.1002/bjs.1800750718
  23. Gupta, S. K., Singh, H., Varshney, A. C. and Parkash, P. : Therapeutic efficacy of honey in infected wounds in buffaloes, Ind., J. Anim., Sci., 62(6) 521-523(1992)
  24. Subrahmanyam, M. : Tropical application of honey in treatment of burns, Br., J. Surg., 78(4), 497-498(1991) https://doi.org/10.1002/bjs.1800780435
  25. Subrahmanyam, M. : Honey-impregnated gauze versus amniotic membrane in treatment of burns. 20(4) 331-333(1994)
  26. Molan, P. C. : The antibacterial properties of honey, Chem. New Zealand, July, 10-14(1995)
  27. Weston, R. J. : Identification and quantiative levels of antibacterial components of some New Zealand Honeys, Food Chem., 70, 427-435(2000) https://doi.org/10.1016/S0308-8146(00)00127-8
  28. Martos, I. Fereres, F. and Thomas-Barberan, F. A. : Identification of flavonoid markers for the botanical origin of Eucalyptus honey, J. Agric. Food Chem., 48, 1498-1502(2000) https://doi.org/10.1021/jf991166q
  29. Andrade, P. : Determination of phenolic compounds in honeys with different floral origin by capillary zone electrophoresis, Food Chem., 62, 79-84(1997)
  30. Martos. I. Francisco, A. and Tomas-Barberan, F. A. : Flavonoid composition of Tunistan Honeys and propolis, J. Agric. Food Chem., 45, 2824-2829(1997) https://doi.org/10.1021/jf9609284