Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Antioxidative activity of flavonoid rich extract of Oenothers odorate Jacquin on oxidation of low density lipoprotein

LDL 산화에 대한 달맞이꽃의 플라보노이드 추출물의 항산화 활성

  • Published : 2002.06.01
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Abstract

There is growing interest ill understanding the role and mechanisms of flavonoid as antioxidant on LDL. The antioxidative activity of flavonoid typically present in Oenothers odorate Jacquin was investigated in vitro using a human LDL oxidation assay. In present work, LDL was incubated with increasing concentrations of extracts of extracts of Oenothers odorate Jacquin and LDL oxidation was started by adding CuSO$_4$to the media. Substances in leaves extracts of Oenothers odorate Jacquin are capable of inhibiting the initiation and the propagation of LDL oxidation. They inhibit LDL oxidation, monitored by thiobarbituric acid-reactive substances(TBARS), as well as modification as shown through direct measurement of electrophoretic mobility, diene conjugates. Inhibition is a dose dependent effect that becomes already apparent at concentration of extracts as low as 40$\mu\textrm{g}$/mL. Inhibition is almost complete at 80$\mu\textrm{g}$/mL. Extracts of Oenothers odorate Jacquin were more potent antioxidative activity than either ascorbic acid and dl-a -tocopherol.

달맞이꽃 추출물로써 항산화활성을 알아보기 위하여 사람 low densty lipoprotein(LDL)에 대하여 실험하였다. 사람 LDL에 CuSO$_4$를 첨가한 후 배양하면서 달맞이꽃 추출을 일정 농도씩 증가시키면서 첨가하여 배양하였다. 달맞이꽃 추출물로서 LDL의 산화에 대한 개시와 연쇄 반응에서 thiobarbituric acid reactivy substances (TBARS)와 전기 영동 및 공액 2중 결합으로 측정하였다. 달맞이꽃 추출물을 LDL에 첨가한 후 macrophage J774로써 배양한 결과 TBARS값은 용량 의존형의 항산화 활성을 나타내었으며 전기 영동 이동상에서도 대조구에 비하여 각 시험구에서 LDL의 산화를 억제하였다. 또 공액 2중 결합에 대한 실험에서 용량 의존형산화 억제 효과를 나타내었다. 달맞이꽃 추출물의 항산화 활성은 40 $\mu\textrm{g}$/ml의 농도에서도 항산화 효과가 있었으나 80$\mu\textrm{g}$/mL의 농도에서는 거의 완전한 억제효과가 있었다. 그리고 비타민 C 및 $\alpha$-dl-tocopherol과 비교한 결과 달맞이들 추출물이 약간 높은 항산화 활성을 나타내었다.

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References

  1. Bonanome, A., A. Pagnan, S. Biffanti, A. Opportuno, F. Sorgato, M. Dorella, M. Maiorino and F. Ursini.1992. Effect of dietary mono unsaturated and poly-unsaturated fatty acids on the susceptibility of plasma low density lipoproteins to oxidative modification. Arteriosder. Thromb. 12, 529-533 https://doi.org/10.1161/01.ATV.12.4.529
  2. Bridges, A. B., N. A. Scoot and J. F. Belch. 1991. Protocol, a superoxide free radical scavenger in vitro. Atherosderosis. 89, 263-265 https://doi.org/10.1016/0021-9150(91)90068-E
  3. Cristol, L. S., I. Jialal and M. Grundy. 1992. Effect of low-dose protocol therapy on LDL oxidation and the plasma lipoprotein profile in male volunteers. Atherosderosis. 97, 10-20
  4. Cominacini, L, U. Garbin, B. Cenci, A. Davoli, E. Ratti, G. Gaviraghi, V. Lo Cascio and A. M. Pastorino. 1991. Predisposition to LDL oxidation during copper-catalyzed oxidative modification and its relation to alpha-tocopherol content in Humans. Clin. Chim. Acta. 204, 57-68 https://doi.org/10.1016/0009-8981(91)90217-Z
  5. Esterbauer, H., H. Puhl, M. Dieber-Rotheneder, G. Waeg and H. Rabl. 1991. Effect of antioxidants on oxidative modification of LDL. Ann. Med. 23, 573-581 https://doi.org/10.3109/07853899109150520
  6. Esterbauer, H., G. Striegl, H. Puhl and M. Rotheneder.1989. Continuous monitoring of in vitro oxidation of human low density lipoprotein. free Radic. Res. Commun. 6, 67-75 https://doi.org/10.1080/08824098909359835
  7. Esterbauer, H., H. Puhl, M. Dieber-Rotheneder, G.Striegl and G. Waeg. 1991. Role of vitamin E in preventing the oxidation of low density lipoprotein. Am. J. Clin. Nutr. 53, 314S-321S
  8. Frankel, E. N., J. Kamer, J. B. German and J. E. Kinsella. 1993. Inhibition of oxidation human lowdensity lipoprotein by phenolic substances in red wine. Lancet. 341, 454-457 https://doi.org/10.1016/0140-6736(93)90206-V
  9. Frei, B., Stocker, R. and B. N. Ames. 1977. Antioxidant defenses and lipid peroxidation in human blood plasma. Proc. Natl. Acad. Sci. USA 85, 9748-9752 https://doi.org/10.1073/pnas.85.24.9748
  10. Greenspan, P. and R. L. Gutman. 1993. Detection by Nile red of agarose gel electrophoresised native and modified low density lipoprotein. Etectrophoresis. 14, 65-68 https://doi.org/10.1002/elps.1150140111
  11. Havel, R. J., H. A. Eder and J. H. Bragdon. 1995. The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. J. CIin. Invest. 34, 1345-1352
  12. Heinecke, J. W., H. Rosen and A. Chait. 1984. Iron and copper promote modification of low density lipoprotein by human arterial smooth muscle cells in culture. J. Clin. Invest. 74, 1890-1894 https://doi.org/10.1172/JCI111609
  13. Henriksen, T., E. Mahoney and D. Steinberg. 1981. Enhanced macrophage degradation of low density lipoportein previously incubated with cultured endothelial cells: Recognition by the receptor for acetylated low density lipoprotein. Proc. Natl. Acad. Sci. USA 78, 6499-6503 https://doi.org/10.1073/pnas.78.10.6499
  14. Jialal, I. and C. Scaccini. 1992. Antioxidants and atherosclerosis. Curr. Opin. Lipidol. 3, 324-328 https://doi.org/10.1097/00041433-199210000-00004
  15. Jiala, I., G. L. Vega and S. M. Grundy. 1990. Physiologic levels of ascorbate inhibit the oxidative modification of low density lipoproteins. Atherosclerosis 82, 185-191 https://doi.org/10.1016/0021-9150(90)90039-L
  16. Lowry, O. H., N. J. Rosebrough, A. L. Farr and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275
  17. Morel, D. W., P. E. Dicorleto and G. M. Chisholm.1984. Endothelial and smooth muscle cells after low density lipoproteins in vitro by free radical oxidation. Arteriosderosis 4, 357-364 https://doi.org/10.1161/01.ATV.4.4.357
  18. Renaud, D. and M. Lorgeril. 1993. Wine, alcohol, platelets and the French Paradox for coronary heart disease. Lancet. 239, 1523-1525
  19. Robak, J., F. Shridi, M. Wolbis, M. Krolikowska and J. Sol. 1988. Screening of the influence of flavonoids on lipoxygenase and cycloxygenase activity as well as on non-enzymatic lipid oxidation. Pharmacol. Pharm. 40, 451-458 https://doi.org/10.1111/j.2042-7158.1988.tb06317.x
  20. Roma, P., A. L. Catapano, S. M. Bertulli, L. Varesi, R.Fumagalli and F. Bernini. 1990. Oxidized LDL increase free cholesterol and fail to stimulate cholesterol esterification in murine macrophages. Biochem. Biophys. Res. 171, 123-131 https://doi.org/10.1016/0006-291X(90)91365-Y
  21. Ross, R. 1993. The pathogenesis of atherosclerosis perspective for the 1990. Nature, 362, 801-809 https://doi.org/10.1038/362801a0
  22. Ryu, B. H., J. W. Jeung, L. G. Robert and P. Greenspan.1990. Antioxidative activitv for human low density lipoprotein oxidation by a novel compound purified form marine microbial origin. J. Marine Biotech. 81, 175-182
  23. Steinbrecher, U., S. Parthasarathy, D. S. Leake, J. L Witztum and D. Steinberg. 1984. Modification of low density lipoprotein by endothelial cells involves lipid peroxidation and degradation of low density lipoprotein phospholipids. Proc. Natl. Acad. Sci. USA 83, 3883-3887
  24. Tallahda, R. J. and R. B. Murray. 1987. Manual of pharmacological calculations with computer programs, 2nd (ed), Springer. Verlag. N. Y, pp. 145-148
  25. Yaki, K. 1976. A simple fluorometric assay for lipoprotein in blood plasma. Biochem. Med. 15, 212-217 https://doi.org/10.1016/0006-2944(76)90049-1