KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Simple/Rapid Method for RNA Preparation from Lactobacillus spp.

Lactobacillus spp.로부터 RNA 추출을 위한 신속/간단한 방법

  • 소재성 (인하대학교 생물공학과, 초정밀 생물분리기술연구센터) ;
  • 오은택 (인하대학교 생물공학과, 초정밀 생물분리기술연구센터) ;
  • 최민지 (인하대학교 생물공학과, 초정밀 생물분리기술연구센터) ;
  • 윤현식 (인하대학교 생물공학과, 초정밀 생물분리기술연구센터)
  • Published : 2002.06.01
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Abstract

Lactobacillus spry. are Gram-positive bacteria playing important roles in human health. In this study, we successfully isolated the total RNA from the cells broken by glass beads using hot phenol method. Moreover, we were able to omit lysozyme and proteinase K treatment by using glass beads to break cell more efficiently. This method was more rapid and simple when compared to the previous one. Prepared RNA can be used for the transcriptional analysis of Lactobacillus spp.

L. crispatus KLB46는 Gram-positive bacteria로써 인간의 건강에 중요한 역할을 한다. 본 연구에서는 glass bead를 이용하여 세포벽을 파괴하고 hot phenol RNA 분리방법을 이용하여 RNA를 성공적으로 분리하였다. 또한 Iysozyme과 proteinase K 처리과정을 배제하여 시간적, 경제적인 면에서 유용한 방법임을 확인할 수 있었다. Gram-positive bacteria에서 glass bead를 이용한 RNA 분리는 특수한 조건에 의해 전사 되거나 반감기가 찬은 mRNA의 연구에 유용한 방법이라 사료된다.

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References

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