The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Characterization of Cucumber mosaic virus Subgroup II Isolated from Paprika (Capsicum annuum var, grossum) in Korea

  • Choi, Gug-Seoun (National Horticultural Research Institute, Rural Development Administration) ;
  • Kim, Jae-Hyun (National Horticultural Research Institute, Rural Development Administration) ;
  • Choi, Jang-Kyung (Division of Biological Environment, College of Agriculture and Life Science, Kangwon National University)
  • Published : 2002.02.01
Download PDF
⟨ Previous Next ⟩

Abstract

An isolate of Cucumber mosaic virus (CMV), PaFMl-CMV causing malformation on the fruit of paprika (Capsicum annuum var, grossum) was characterized based on biological reactions, serological relationships, and partial nucleotide sequence analyses. PaFMl-CMV was distinguishable from other isolates of CMYI Mf-(subgroup I) and LS-CMV (subgroup II), in terms of its reactions to some host plants. Polyclonal antibody against PaFMl-CMV showed homologous antigenic relationship with LS-CMV, however, the antibody formed a spur between PaFMl- and Mf-CMV, In the comparison of molecular size of dsRNAs of PaFMl-CMV with Mf- and LS-CMV, PaFMl-CMV had a slightly smaller RNAl and larger RNA2, RNA3, and RNA4. When the CDNA product of PaFMl-CMV coat protein (CP) gene was digested with some restriction enzymes, the fragment pattern was identical with that of LS-CMV The nucleotide and amino acid sequences of PaFMl-CMV CP gene were 99.5% and 98.6% identical with LS-CMV respectively. The data indicate that PaFMl-CMV belongs to subgroup II of CMV, which is the first report in Korea.

Keywords

References

  1. Choi, J. K., Kim, H. J., Hong, J. S., Kim, D. W. and Lee, S. Y. 1998. Identification and differentiation of cucumber mosaic virus isolates in Korea. Korean J. Plant PathoI. 14:7-12
  2. Choi, H. S., Ryu, J. K., Ahn, K. K., Cho, J. D. and Kim, J. S. 2001. Cucumber mosaic Cucumovirus-CARNA5 causing bud necrosis on table tomato. PIant Pathol. J. 17:169-173
  3. Choi, S. K., Choi, J. K., Park, W. M. and Ryu, K. H. 1999. RTPCR detection and identification of three species of cucumovirus with a genus specific single pair of primers. J. ViroI. Methods 83:67-73 https://doi.org/10.1016/S0166-0934(99)00106-8
  4. Davies, C. and Symons, R. H. 1988. Further implication for the evolutionary relationship between triparticle plant viruses based on cucumber mosaic virus RNA3. ViroIogy 165:216-224
  5. Edwards, M. C. and Gonsalves, D. 1983. Grouping of seven biological defined isolates of cucumber mosaic virus by peptide mapping. Phytopathology 73:1117-1120 https://doi.org/10.1094/Phyto-73-1117
  6. Francki, R. I. B., Randles, J. W., Chambers, T. C. and Wilson, S. B. 1966. Some properties of purified cucumber mosaic virus (Q strain). Virology 28: 729-741 https://doi.org/10.1016/0042-6822(66)90257-1
  7. Gonda, T. J. and Symons, R. H. 1978. The use of hybridization analysis with complementary DNA to determine the RNA sequence homology between strains of plant viruses: Its application to several strains of cucumoviruses. ViroIogy 88:361-370
  8. Laemmli, U. K. and Favre, M. 1973. Maturation of the head of bacteriophage T4. I. DNA packaging event. J. Mol. Biol. 80:575-599 https://doi.org/10.1016/0022-2836(73)90198-8
  9. Morris, T. J. and Dodds, J. A. 1979. Isolation and analysis Of double- stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69:854-858 https://doi.org/10.1094/Phyto-69-854
  10. Mossop, D. W., Francki, R. I. B. and Gribvell, C. J. 1976. Comparative studies on tomato aspermy and cucumber mosaicviruses. V. Purification and properties of a cucumber mosaic virus inducing severe chlorosis. Virology 74:544-546 https://doi.org/10.1016/0042-6822(76)90361-5
  11. Nitta, N., Masuta, C., Kuwata, S. and Takanami, Y. 1988. Inoculation with RNAs 1 and 2 of cucumber mosaic virus induces viral RNA replicase activity in tobacco mesophyll protoplasts. J. Gen. Virol. 69:2695-2700 https://doi.org/10.1099/0022-1317-69-10-2695
  12. Ouchterlony, O. 1968. Handbook of immunodiffusion and immunoelectrophoresis. Prog. Allergy. Ann. Arbor Sci. Publ. 135 p
  13. Owen, J., Shintaku, M., Aeschleman, P., Tahar, S. B. and PaIukaitis, P. 1990. Nucleotide sequence and evolutionary relationship cucumber mosaic virus (CMV) strains: CMV RNA3. J. Gen. ViroI. 11:2243-2249
  14. Palukaitis, P., Roossinck, M. J., Dietzgen, R. G. and Francki, R. I. B. 1992. Cucumber mosaic viras. Adv. Virus Res. 41:281-348 https://doi.org/10.1016/S0065-3527(08)60039-1
  15. Quemada, H., Keaey, C., Gonsalves, D. and Slightom, J. L. 1989. Nucleotide sequence of the coat protein genes and flanking region of cucumber mosaic virus strain C and WL RNA3. J. Gen. ViroI 70:1065-1073 https://doi.org/10.1099/0022-1317-70-5-1065
  16. Rizos, H., Gunn, L. V., Pares, R. D. and Gillings, M. R. 1992. Differentiation of cucumber mosaic virus isolates using the polymerase chain reaction. J. Gen. Virol. 73:2099-2103 https://doi.org/10.1099/0022-1317-73-8-2099
  17. Takanami, Y. 1981. A striking change in symptoms on cucumber mosaic virus-infected tobacco plants induced by a satellite RNA. Virology 109:120-126 https://doi.org/10.1016/0042-6822(81)90476-1
  18. Shintaku, M. and Palukaitis, P. 1990. Genetic mapping Of cucumber mosaic virus. In Viral Genes and PIant Pathogenesis, ed. by Pirone, T. P. and Shaw, J. G., pp. 157-164, Spriger-Verlag, New York
  19. Wahyuni, W. S., Dietzgen, R. G., Hanada, K. and Francki, R. I. B. 1992. Serological and biological variation between and within subgroup I and II strains of cucumber mosaic virus. Plant Pathol. 41:282-297 https://doi.org/10.1111/j.1365-3059.1992.tb02350.x

Cited by

  1. Expression of Cucumber Mosaic Virus Coat Protein and Its Assembly into Virus Like Particles vol.1, pp.3, 2014, https://doi.org/10.17795/bhs-24729
  2. Outbreak of Cucumber mosaic virus and Tomato spotted wilt virus on Bell Pepper Grown in Jeonnam Province in Korea vol.24, pp.1, 2008, https://doi.org/10.5423/PPJ.2008.24.1.093
  3. CMV-P1 Resistance Evaluation Using Enzyme-linked Immunosorbent Assay of Pepper Genetic Sources (Capsicum spp.) vol.31, pp.6, 2013, https://doi.org/10.7235/hort.2013.13021
  4. Characterization ofSclerotinia sclerotiorumIsolated from Paprika vol.34, pp.3, 2006, https://doi.org/10.4489/MYCO.2006.34.3.154
  5. First Report of Tobacco mild green mosaic virus Infecting Pepper in Korea vol.18, pp.6, 2002, https://doi.org/10.5423/PPJ.2002.18.6.323
  6. Characterization ofFusarium oxysporumIsolated from Paprika in Korea vol.35, pp.2, 2007, https://doi.org/10.4489/MYCO.2007.35.2.091