미생물학회지 (Korean Journal of Microbiology)

  • 제38권2호
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  • Pages.113-118
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  • 2002
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  • 0440-2413(pISSN)
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  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Denitrifying Phosphate Accumulating Organisms (dPAOs)을 이용한 영양소제거 및 반응조내 미생물 분포 조사

Nutrient Removal using the Denitrifying Phosphate Accumulating Organisms (dPAOs) and Microbial Community Analysis in Anaerobic-Anoxic Sequencing Batch Reactor

  • 박용근 (고려대학교 생명공학원) ;
  • 이진우 (고려대학교 환경시스템 공학과) ;
  • 이한웅 (고려대학교 생명공학원) ;
  • 이수연 (고려대학교 생명공학원) ;
  • 최의소 (고려대학교 토목·환경공학과)
  • 발행 : 2002.06.01
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⟨ 이전 논문 다음 논문 ⟩

초록

혐기-무산소조건으로 구성된 회분식 반응조에서 질산염을 이용하여 인(P)도 동시에 제거될 수 있는 가능성을 알아보기 위해서 인의 제거 양상을 혐기-호기조건의 반응조와 비교하여 조사하였고, 질산염과 인을 동시에 제거하는 미생물분포를 분석하였다. 그 결과 비교적 낮은 농도의 유기물이 적용되었을 때(평균 CODcr=130mg/ι)두 반응조 모두 인이 효과적으로 제거되었으며 반응조내의 최종 인의 농도를 1 mg P/L. 이하로 유지하였다. 특히, 질산염을 전자수용체로 이용한 혐기-무산소조건의 반응조는 기존의 영양소제거 시스템과 비교하여 5-7 mg (P+N)/ι의 영양소를 추가적으로 제거하여 유기물의 효과적 인 이용이 가능한 것으로 판명되었다. 혐기-무산조 조건의 방응조내 미생물 분포를 조사 한 결과 질소원을 제거하는 미생물군(denitrifying bacteria)과 인을 제거하는 미생물군(polyphosphate accumulating bacteria)이 함께 존재하고 있음이 밝혀졌고, 이들 중 $\beta$-proteobacteria에 속하는 Zoogloea ramigera와 Rhodocyclus에 포함되는Alcaligenes defragrans 등은 탈질능력 이 있으면서 anoxic상태에서 인을 동시에 축적할 수 있는 탈질-탈인균주(denitrifying phosphate accumulating organisms; dPAO)로 조사되었다.

Laboratory experiments were aimed to evaluate the effect of nitrate as a electron acceptor during the biological phosphorus uptake and to investigate the microbial community. Anaerobic-anoxic sequencing batch reactor (SBR) compared the removal behaviour to anaerobic-oxic SBR, both SBRs maintained lower effluent quality with 1.0 mgp/1. Anaerobic-anoxic SBR was able to remove additional 5.0 to 7.0 mg (P+N)/ι than other biological nutrient removal (BM) system. Therefore, it was proposed that the anaerobic-anoxic SBR was more effective at weak sewage. From the results of the maicrobial community analysis, it can be inferred that denitrifying bacteria and polyphosphate accumulating bacteria coexist in anaerobic-anoxic SBR during stable condition for removing the nitrogen and phosphorus. Particularly, it was suggested that the Zoogloea ramigera in the $\beta$-subclass of proteobacteria and the Alcaligenes defragrans of the Rhodocyclus group in the $\beta$-subclass of proteobacteria played a major role for removing the nitrogen and phosphorus as dPAOs (denitrifying phosphate accumulating organisms).

키워드

참고문헌

  1. 1998년 한국 수질보전 학회 학술연구 발표회 논문 초록집(고려대학교) v.33 Autothrophic denitrification using sulfide 이진우;최의소
  2. Wat. Sci. Tech. v.23 The effect of organic compound on biological phosphorous removal Abu-ghararah, Z.H.;C.W. Randall
  3. Microbiol. Rev. v.59 Phylogenetic identification and in situ detection of individual microbial cells without cultivation Amann, R.;I.W. Ludwig;K.H. Schleifer
  4. Standard Methods for Examination of Water & Wastewater(19th Ed.) APHA
  5. Appl. Environ. Microbiol. v.61 Bacterial community structures of phosphate-removing and non-phosphate-removing activated sludges from sequencing batch reactors Bond, P.L.;P. Hugenholtz;J. Keller;L. Blackall
  6. Proc. Natl. Acad. Sci. v.75 Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli Brosius, J.;J.L. Palmer;H.P. Kennedy;H.F. Noller https://doi.org/10.1073/pnas.75.10.4801
  7. Wat. Sci. Tech. v.34 Another Carbon Source for BNR System Choi, E.;H.S. Lee;J.W. Lee;S.W. Oa
  8. Wat. Sci. Tech. v.43 Phosphorus removal from SBR with controlled denitrification for weak sewage Choi, E.;H. Park;D. Rhu
  9. Appl Microbiol Biotechnol v.49 Metabolic transformations and characterisation of the sludge community in an enhanced biological phosphorus removal system Christensson, M;L. Blackall;T. Welander https://doi.org/10.1007/s002530051163
  10. Appl. Envir. Microbiol. v.66 Identification of polyphosphate-accumulating organisms and design of 16S rRNA-directed probes for their detection and quantitation Crocetii, R.G;P. Hugenholtz;P.L. Bond;A. Schuler;J. Keller;D. Jenkins;L. Blackall https://doi.org/10.1128/AEM.66.3.1175-1182.2000
  11. Am. Soc. Microbiol. Press. Microbiology Manual of methods for general bacteriology Gerhardt, P;R.G.E. Murrary;R.N. Costilow;E.W. Nester;W.A. Wood;N.R. Krieg;G.R. Pillips
  12. Appl. Envir. Microbiol. v.65 PCR detection of genes encoding nitrite reductase in denitrifying bacteria Hallin, S.;P-E. Lindgren
  13. Appl. Environ. Microbiol. v.58 Dual staining of natural bacterioplankton with 4',6-diamidino-2-phenylindole and fluorescent oligon-ucleotide probes targeting kingdom-level 16S rRNA sequences Hicks, R.E.;R.J. Amann;D.A. Stahl
  14. Bergey's manual of determinative bacteriology(9th ed.) Holt, J.G.;N.R. Krieg;P.H.A. Sneath;J.T. Stalely;S. T. Williams
  15. Wat. Res. v.33 In situ identification of polyphosphate accumulating bacteria in activated sludge by dual staining with rRNA-targeted oligo-nucleotide probes and 4',6-diamidino-2-phenylindol (DAPI) at polyphosphate-probing concentration Kawaharasaki, M.;H. Tanaka;T. Kanagawa;K. Nakamura https://doi.org/10.1016/S0043-1354(98)00183-3
  16. FEMS Microbiol Rev. v.15 Biology of polyphosphate-accumulating bacteria involved in enhanced biological phosphorus removal Kortstee G.J.J;K.J. Appeldorn;C.F.C. Bonting;E.W.J. Niel van;H.W. Veen van https://doi.org/10.1111/j.1574-6976.1994.tb00131.x
  17. Appl. Environ. Microbiol. v.62 Estimation of the abundance of an unculturable soil bacterial strain by a competitive quantiative PCR method Lee, S.Y.;J.B. Bollinger;D. Bezdicek;A. Ogram
  18. J. Mol. Biol. v.53 Calcium dependent bacteriophage DNA infection Mandel, M.;A. Higa
  19. Syst. Appl. Microbiol. v.15 Phylogenetic oligonucleotide probes for the major subclasses of Proteobacteria: problems and solutions Manz, W.;R. Amann;W. Ludwig;M. Wagner;K.H. Schleifer https://doi.org/10.1016/S0723-2020(11)80121-9
  20. Wat. Sci. Tech. v.39 Characterization of the denitrifying fraction of phosphate accumulating organisms in biological phosphate removal Meinhold, J.;C. Filipe;G. Daigger;S. Isaacs
  21. Curr. Microbiol. v.38 Polyphosphate-accumulating and denitrifying bacteria isolated from anaerobic-anoxic and anaerobic-aerobic sequencing batch reactors Merzouki, M.;J.P. Delgenes;N. Bernet;R. Moletta;M. Benlemlih https://doi.org/10.1007/PL00006776
  22. Int. J. Syst. Bacteriol. v.45 Microlunatus phosphorus gen. nov., sp. nov., a new gram-positive polyphosphate-accumulating bacterium isolated from activated sludge Nakamura K.;A. Hiraishi;Y. Yoshimi;M. Kawaharasaki;K. Masuda;Y. Kamagata https://doi.org/10.1099/00207713-45-1-17
  23. Molecular cloning: A laboratory manual Sambrook, J.;E.F. Fritsch;T. Maniatis
  24. Appl. Environ. Microbiol. v.58 Isolation and characterization of a nitrite-reduction gene and its use as a probe for denitrifying bacteria Simith, G.B.;J.M. Tiedje
  25. Appl Environ Microbiol. v.60 Development of an rRNA-targeted oligonucleotide probe specific for the genus Acinetobacter and its application for in situ monitoring in acticated sludge Wagner M.;R. Erhart;W. Manz;R. Amann;H. Lemmer;D. Wedi;K.H. Schleifer