KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Extraction Method of Carotenoios from Rhodotorula glutinis

Rhodotorula glutinis로 부터 Carotenoios의 추출방법

  • 김의용 (서울시립대학교 공과대학 화학공학과) ;
  • 박평규 (서울시립대학교 공과대학 화학공학과)
  • Published : 2002.02.01
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Abstract

An efficient method of extraction for carotenoids in Rhodotorula glutinis KCTC 7989 was developed. Major carotenoids produced were identified as torularhodin of 61.7%, $\beta$-carotene of 28.8%, and torulene of 9.5%. HCI treatment, as a pretreatment on cell, was necessary to carry out together with thermal treatment unlike DMSO pretreatment. The choice of solvent had an important effect on the composition of carotenoids extracted: benzene and chloroform were effective for the extraction of torularhodin, especially. However, diethyl ether was most effective for the extraction of total carotenoids. Freeze dried type cells showed high efficiency value for the extraction of carotenoids, in compared with dried and wet type cells.

Rhodotorula giutinis KCTC 7989내에 존재하는 카로티노이드를 효율적으로 추출하기 위한 방법에 대해 연구하였다. R. glutinis에 의해 생합성되는 카로티노이드의 조성은 torularhodin 61.7%, $\beta$-carotene 28.8%, torulene 9.5%이었다. 세포 내에 존재하는 카로티노이드를 추출하기 위해 HCI로 세포를 전처리할 경우 열처리의 병행이 중요한 인자였지만, DMSO를 사용한 경우는 열처리 유무에 큰 영향을 받지 않았다. 추출용매에 따라 추출되는 카로티노이드의 조성이 영향을 받았다. 특히 벤젠과 클로로포름은 torularhodin의 추출에 효과적이었으며, 디에틸에테르를 사용했을 때 카로티노이드의 전체 추출농도가 가장 높았다. 건조된 세포나 습한 세포에 비해 냉동 건조된 세포에서 카로티노이드의 추출효율이 높게 나타났다.

Keywords

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