상온보관이 가능한 건조체 명태의 DNA size marker

Development of the Method Allowing DNA Size Markers to be Ambient Storage with Lyophilized Type

  • 전복환 (대구광역시 달서구 대천동 739 (주)제일생명공학서비스 세포공학연구소) ;
  • 강성원 (대구광역시 달서구 대천동 739 (주)제일생명공학서비스 세포공학연구소) ;
  • 서정원 (대구광역시 달서구 대천동 739 (주)제일생명공학서비스 세포공학연구소) ;
  • 이규식 (대구광역시 달서구 대천동 739 (주)제일생명공학서비스 세포공학연구소) ;
  • 조유진 (대구광역시 달서구 대천동 739 (주)제일생명공학서비스 세포공학연구소) ;
  • 박종구 (대구광역시 중구 동산동 계명대학교 동산의료원 일반외과/의과학연구소)
  • 발행 : 2002.02.01

초록

Gel electrophoresis of DNA is a well known technique in molecular biology. This technique is simple, rapid to perform, and capable of adequately separating fragments of DNA. A number of mixtures of DNA fragments ("DNA size markers") are frequently employed in a purpose of extrapolating the sizes or the amount of DNA molecules during gel electrophoresis. DNA size markers are constructed by digesting plasmid DNA, bacteriophage DNA, or recombinant DNA molecules with one or more restriction enzymes. However, liquid suspension containing DNA size marker needs to be kept at a low temperature during storage and shipping. In an attempt to maintain the DNA samples at room temperature for extended period of time, lyophilization of DNA with addition of nuclease inhibitor was studied. Gel loading buffer was also added to the lyophilized DNA to provide additional convenience such that DNA size marker was the "ready-to-use" followed by simply reconstituting with distilled water.

키워드

참고문헌

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