Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Effect of Bovine Somatotropin (bST) Treatment on Embryo Recovery and Pregnancy Rate in Hanwoo

한우에서 bST 처리가 수정란 회수 및 수태율에 미치는 영향

  • Jung, S. H. (Department of Animal Science, College of Agriculture and Life Sciences, Sunchon National University) ;
  • Lee, J. W. (Department of Animal Science, College of Agriculture and Life Sciences, Sunchon National University) ;
  • Son, B. H. (Department of Animal Science, College of Agriculture and Life Sciences, Sunchon National University) ;
  • Go, J. S. (Joennam Institute of Livestock & Veterinary Science) ;
  • Mun, M. (Joennam Institute of Livestock & Veterinary Science) ;
  • Cho, S. S. (Joennam Institute of Livestock & Veterinary Science) ;
  • Choi, S. B. (Namwon Branch National Livestock Research Institute, R.D.A) ;
  • Son, S. G. (Namwon Branch National Livestock Research Institute, R.D.A) ;
  • Jeong, G. I. (Namhae Artificial Insemination Center) ;
  • Bae, I. H. (Department of Animal Science, College of Agriculture and Life Sciences, Sunchon National University) ;
  • Cho, S. G. (Department of Animal Science, College of Agriculture and Life Sciences, Sunchon National University) ;
  • Kong, I. K. (Department of Animal Science, College of Agriculture and Life Sciences, Sunchon National University)
  • 정세환 (순천대학교 농업생명과학대학 동물자원과학과) ;
  • 이정우 (순천대학교 농업생명과학대학 동물자원과학과) ;
  • 손병훈 (순천대학교 농업생명과학대학 동물자원과학과) ;
  • 고재상 ;
  • 문만 ;
  • 조상신 ;
  • 최성복 ;
  • 손삼규 ;
  • 정경일 ;
  • 배인휴 (순천대학교 농업생명과학대학 동물자원과학과) ;
  • 조성균 (순천대학교 농업생명과학대학 동물자원과학과) ;
  • 공일근 (순천대학교 농업생명과학대학 동물자원과학과)
  • Published : 2002.04.01
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Abstract

The purpose of this study was to determine the effect of bST treatment on embryo recovery and pregnancy rate following embryo transfer. Donor cows were superovulated with Folltropin-V and PGF$_2$$\alpha$ combination method and then inseminated with frozen semen 3 times 12 hrs interval. Donor and recipient cows were assigned to control and bST group, of which was given a single injection of bST (500 mg, im) at insemination or estrus detection. Embryo collection of superovulated cows were flushed nonsurgical method at 7 to 8 days after artificial insemination. The percentare and Mean$\pm$S.E. of transferable embryo was not significantly different between control and bST treatment (72.8%/5.9$\pm$4.5 vs. 83.7%15.1 $\pm$ 1.6). The percentage and Mean$\pm$S.E. of transferable embryo in non-summer season was significantly higher than in summer (81.8%/5.4$\pm$2.1 vs. 68.7%14.774.6; P<0.05). The pregnancy rate after embryo transfer in bST treatment was significantly higher than in control (64.0 vs. 47.1%; P<0.05). There was no significant difference in pregnancy rate between summer and non-summer (51.6 vs. 61.5%; P>0.05). The results indicated that InST treatment in recipient cows could improve the efficiency of transferable embryo production and pregnancy rate after embryo transfer, and non-summer season may be better far superovulation treatment and embryo transfer.

본 연구는 과배란처리에 의한 수정란이식 시 우수한 수정란을 다량 확보하고 이식 후 수태율 향상을 위하여 공란우 및 수란우에 bST처리가 수정란회수를 및 수태율에 어떠한 영향을 미치는가를 조사하고자 실시하였다. 공란우는 Folltropin-V와 PGF$_2$$\alpha$를 이용하여 과배란처리를 유도하여 12시간 간격으로 2 straw치 3회 인공수정을 실시하였다. 공란우와 수란우는 대조구와 bST처리구로 구분하였으며, bST(500 mg)처리는 발정발현 후 미근부에 근육주사하였다. 과배란처리된 공란우의 수정란배란을 수정 후 7~8일째에 비와과적인 방법으로 실시 하였다. 이식가능 수정란의 회수율과 평균숫자는 대조구와 bST처리구 간에 유의적인 차이가 없었다 (72.8%/5.9$\pm$4.5 vs. 83.7%/5.1 $\pm$1.6; P<0.05). 비여름철에 이식가능 수정란의 회수율과 평균숫자가 여름철보다 유의적으로 높았다(81.8%15.4$\pm$2.1 vs. 68.7%14.7$\pm$4.6; P<0.05). 수정란이식 후 수태율은 bST처리구에서 대조구보다 유의적으로 높았다 (64.0 vs. 47.1%; P<0.05). 그러나 이식 후 수태율에 계절간에 유의적인 차이는 없었다(51.6 vs.61.5%; P>0.05). 본 연구결과에서 bST처리는 수정란이식 후 수태율의 향상시킬 수 있을 것으로 판단되며, 비여름철이 과배란처리 및 수정란이식에 유리할 것으로 판단된다.

Keywords

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