Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Evaluation of Fertilizer Additions to Stimulate Oil Biodegradation in Sand Seashore Mesocosms

  • CHOI, SUNG-CHAN (Microbiology Laboratory, Korea Ocean Research and Development Institute, Division of Life Sciences, Hallym University) ;
  • KAE KYOUNG KWON (Microbiology Laboratory, Korea Ocean Research and Development Institute) ;
  • JAE HAK SOHN (Microbiology Laboratory, Korea Ocean Research and Development Institute) ;
  • SANG-JIN KIM (Microbiology Laboratory, Korea Ocean Research and Development Institute)
  • Published : 2002.06.01
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Abstract

Effects of fertilizer additions for oil degradation were examined in sand seashore mesocosms. Within 37 days, up to $85\%$ removal was achieved by the addition of slow-release type fertilizer (SRF) with the initial degradation rate of 423.3 mg oil $(kg sand)^-1\;day^-1$. The removal was mostly of biological origin based on the changes of $C_17$ /pristane and $C_18$/phytane ratios from 2.60 to 0.81 and from 3.55 to 1.29, respectively. The addition of oleophilic fertilizer (Inipol EAP22) was less effective and resulted in the removal of $64\%$ of the added oil ($3\%$, v/v) with a lower initial degradation rate. Petroleum-degrading bacteria had achieved a value of $1{\times}10^8$ CFU $(g sand)^-1$ at Day 3 and this peak exactly coincided with the initial degradation in the SRF-treated mesocosm. In this mesocosm, surface tension values were decreased drastically during Days 3 and 8, suggesting that microbially-produced surface-active agents actively enhanced the oil degradation rate and cell proliferation. Although the Inipol-treated mesocosm appeared to show significantly enhanced oil degradation compared to that of the untreated control mesocosm, Inipol was found to be less effective than SRF in enhancing a true oil-degrader when compared under similar experimental conditions.

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