Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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G$\alpha$12 and G$\alpha$13 Subunits Modulate $Ca^{2+}$-Induced Histamine Release in Human Umbilical Cord Blood-Derived Mast Cells

  • Ro, Jai-Youl (Department of Pharmacology, School of Medicine, Sungkyunkwan University) ;
  • Kim, Ji-Young (Department of Pharmacology, School of Medicine, Sungkyunkwan University) ;
  • Ha, Ji-Hee (Department of Pharmacology and Institute of Biomedical Sciences, Collegeof Medicine, Hanyang University) ;
  • Lee, Chang-Ho (Department of Pharmacology and Institute of Biomedical Sciences, Collegeof Medicine, Hanyang University)
  • Published : 2002.06.01
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Abstract

The role of $G{\alpha}12\;and\;G{\alpha}13$ in modulating the IgE receptor-mediated histamine secretion in the streptolysin-o-permeabilized human cultured mast cell was investigated. The expression of $G{\alpha}12\;and\;G{\alpha}13$ proteins were regulated during human cultured mast cell differentiation, and a significant correlation was observed between the levels of expression of $G{\alpha}12\;and\;G{\alpha}13$ proteins and IgE receptor-mediated histamine secretion capability in human cultured mast cells. Antibodies against $G{\alpha}12\;and\;G{\alpha}13$ effectively inhibited the IgE receptor-induced histamine release, and the concentration of anti-$G{\alpha}12$ antibody used to inhibit histamine secretion was shown to also inhibit the IgE receptor-mediated elevation of intracellular $Ca^2+$. Therefore, the results suggest that $G{\alpha}12\;and\;G{\alpha}13$ play roles in modulating IgE receptor-activated $Ca^2+$ influx, thereby regulating histamine release in cultured human mast cells. This is the first report to show that $G{\alpha}12\;and\;G{\alpha}13$ are involved in the regulation of $Ca^2+$ mediated exocytosis in human cultured mast cells.

Keywords

References

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