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Effect of Pretreatment Conditions on Effective Components of Extracts from Safflower (Carthamus tinctorius L.) Seed

전처리조건이 홍화씨 추출물의 유효성분 함량에 미치는 영향

  • Kim, Jun-Han (Dept. of Food Science & Nutrition, Sangju National University) ;
  • Park, Jun-Hong (Uisong Medicinal Plant Experiment Station, Kyungpook Provincial A.T.A.) ;
  • Kim, Jong-Kuk (Dept. of Food Science & Nutrition, Sangju National University) ;
  • Lee, Jin-Man (Dept. of Herbs and Food Science, Kyongbuk College of Science) ;
  • Moon, Kwang-Deog (Dept. of Food Science & Technology, Kyungpook National University)
  • 김준한 (상주대학교 식품영양학과) ;
  • 박준홍 (경북농업기술원 의성약초시험장) ;
  • 김종국 (상주대학교 식품영양학과) ;
  • 이진만 (경부과학대학 약용식품과) ;
  • 문광덕 (경북대학교 식품공학과)
  • Published : 2002.06.01

Abstract

In order to utilize safflower seed effectively as a food material, it was processed at the conditions including roasting temperature/time of 170$\^{C}$/10 min to 210$\^{C}$/30 min, ethanol concentration of 0 to 100% (V/V) and enzyme hydrolysis with $\alpha$-amylase, $\beta$-amylase, amyloglucosidase and cellulase. Safflower seed extracts had the highest soluble solid content at the condition of 60% ethanol concentration, roasting at 190$\^{C}$ for 20 min and hydrolysis with amyloglucosidase. Total phenolic compounds increased with the ethanol concentration, showing the highest at the condition of 80% ethanol, roasting at 170$\^{C}$ for 30 min and hydrolysis with amyloglucosidase. High level total flavonoid was observed at the condition of 80% ethanol, roasting at 210$\^{C}$ for 30 min and hydrolysis with amyloglucosidase. Safflower seed had sucrose as major free sugar as well as xylose and arabinose as minor free sugars. Organic acids in safflower seed included oxalic, citric, magic and fumaric acid. Serotonin I (N-[2-(5-hydroxy-1H-indo-1-3-yl)ethyl]ftrulamide) and serotonin II (N-[2-(5-hydroxy-1H-indol-3yl)ethyl]-p-coumaramide) as antioxidant compounds increased with ethanol concentration, showing the highest revel at 60% ethanol. Acacetin content increased with temperature and roasting time, with a maximum of 69.47 mg% at 210$\^{C}$ for 30 min.

홍화씨의 식품재료적 가치를 높이는 기초적 연구의 일환으로 추출용매와 볶음조건의 확립 및 유효성분 등을 분석한 결과는 다음과 같다. 전처리조건에 따른 홍화씨 추출물의 고형분 함량은 60% ethanol 농도, 19$0^{\circ}C$에서 20분간 볶음 처리 구 및 amylogucosidase 처리구에서 매우 높은 함량의 고형분이 추출되었다. 총페놀 함량은 ehtanol의 농도가 증가함에 따라 큰 증가를 보였고 ethanol 농도 80%, 17$0^{\circ}C$, 30분과 21$0^{\circ}C$, 30분처리구 및 amyloglucosidase 처리구와 celluase 처리구에서 매우 높은 함량을 나타내었다. 총 flavonoid 함량은 80% ethanol 처리구, 21$0^{\circ}C$, 30분과 21$0^{\circ}C$, 20분 처리구 및 amyloglucosidase와 cellulase 처리구에서 매우 높은 함량을 나타내었다. 유리당 조성은 sucrose가 주요 당이었고 xylose와 araginose가 미량으로 확인되었으며, 주요 유기산으로는 citric, oxalic, malic 및 fumaric acid가 확인되었고, 60% ethanol처리구에서 대체적으로 많은 양의 유기산을 함유하고 있었다. 항산화 효과를 가지고 있는 serotonin 유도체인 serotonin(N-[2-(5-hydroxy-1H-indol-3-yl) ethyl]ferulamide)과 serotonin II(N-[2-(5-hydroxy-1H-indol-3yl)ethyl]-p-coumaramide)의 함량은 ethanol의 농도가 증가함에 따라 높은 증가를 나타내었고, 특히 60% ethanol 용액에서 매우 높은 함량을 보였다. Acacetin의 함량은 볶음 온도와 시간의 증가에 따라 대체로 큰 폭으로 증가하였고 21$0^{\circ}C$, 30분 처리구에서 69.47mg%로 가장 높은 함량을 나타내었다.

Keywords

References

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