Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Dandelion on Oxygen Free Radical Generating and Scavenging System of Brain in Streptozotocin-Induced Diabetic Rats

서양민들레가 Streptozotocin으로 유발한 당뇨 횐쥐의 뇌조직 중 유해 활성산소 생성 및 제거 효소계에 미치는 영향

  • 김명주 (대구산업정보대학 식품영양과) ;
  • 조수열 (영남대학교 식품영양학과)
  • Published : 2002.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Many studies have shown that hyperglycemia leads to an increase of lipid peroxidation in diabetic patients and animals, reflecting a rise reactive oxygen species production. It is increasingly recognized that brain is another site of diabetic organ damage. Accordingly, this study was to investigate the effect of dandelion on oxygen free radical generating and scavenging system of brain in streptozotocin (STZ)-induced diabetic rats. Male Wistar rats were divided into diabetic (control) and diabetic-dandelion supplemented groups. Dandelion was supplemented for 4 weeks with dandelion leaf and root powder (DLP, DRP) or dandelion leaf and root water extract (DLW, DRW) based on 11.4 g of raw dandelion/kg diet. Diabetes was induced by single injection STZ (55 mg/kg B.W., i.p.)in a citrate buffer. Oxygen free radical generating enzymes, cytochrome P-450, amino-pyrine N-demethylase, aniline hydroxylase and xanthine oxidase, were lowered in dandelion supplemented-groups compared to the control group. Superoxide dismutase, catalase and gluthathione peroxidase activities of brain were also lower in dandelion leaf and root supplemented-group than in the control group, whereas glutathione S-transferase activity and gluthathione content were increased in dandelion supplemented-groups compared to the control group. With regard to the lipid peroxidation products, the malondialdehyde content of brain was lower in dandelion supplemented groups. Therefore, it could be suggested that powder and water extract of dandelion leaf or root are beneficial in preventing diabetic complication from lipid peroxidation and free radical in brain of diabetic rat brain.

뇌조직에서 고혈당으로 인한 산화적 스트레스에 대한 민들레의 유해 활성산소 생성과 제거효소계에 미치는 영향을 알아보고자 streptozotocin으로 당뇨를 유발한 Wistar계 횐쥐에게 민들레의 잎과 뿌리의 분말과 열수추출물을 각각 4주간 급여하였다. 실험결과 유해 활성산소 생성효소계인 시토크롬 P450 함량, aminopyrine N-demethylase, aniline hydroxylase 및 xanthine oxidase 활성은 당뇨를 유발한 대조군에 비하여 서양민들레 잎과 뿌리 급여군 모두 감소되었다. Superoxide dismutase, catalase와 glutathione peroxidase 활성 역시 서양민들레의 분말과 열수추출물 급여시 대조군에 비하여 유의적인 감소를 나타내었으며 민들레의 부위에 따른 차이는 관찰되지 않았다. 반면 뇌조직 중의 glutathione S-transferase 활성과 GSH 함량은 대조군에 비 하여 서양민들레 급여시 유의적으로 증가되었으며, 과산화지질 함량은 당뇨 대조군에 비하여 서양민들레 급여군 모두 유의적인 감소를 보였다. 이상의 결과에서 서양민들레의 잎과 뿌리의 급여는 당뇨로 인한 횐쥐의 뇌조직 중 유리기 생성과 지질과산화로 인한 합병증 예방에 효과적일 것으로 사료된다.

Keywords

References

  1. Dario G, Antonio C, Giuseppe P. 1996. Oxidative stress and diabetic vascular complications. Diabetes Care 19: 257-267 https://doi.org/10.2337/diacare.19.3.257
  2. Wohaieb SA, Godin DV. 1987. Alteration in free radical tissue-defense mechanism in streptozotocin-induced dia-betes in rat. Diabetes 36: 1014-1019 https://doi.org/10.2337/diabetes.36.9.1014
  3. Enghofer M, Usadel KH, Beck OB, Kusterer K. 1997. Superoxide dismutase reduces islet microvascular injury induced by streptozotocin in the rat. Am J Physiol 273: E376-E382
  4. Camiron NE, Cotter MA. 1995. Neurovascular dysfunction in diabetic rats: Potential contribution of autooxidation and free radicals examined using transition mental chelating agents. J Clin Invest 96: 1159-163 https://doi.org/10.1172/JCI118104
  5. Biessels GJ, Kappelle AC, Bravenboer B, Erkelens DW, Gispen WH. 1994. Cerebral function in diabetes mellitus. Diabetologia 37: 643-650 https://doi.org/10.1007/BF00417687
  6. Emert J, Chaudiere J. 1989. Free radicals and lipid per oxidation in cell pathology. In CRC Handbook of free rad-icals and antioxidants in biomedicine. Miquel J, Quintaniha AT, Weber H, eds. CRC press Inc, Miami. p 178-179
  7. Acker W, Aps E, Majumdar SK, Shaw G, Thomson AD. 1982. The relationship between brain and liver damage in chronic alcoholic patients. J Neur Neurosurg Psych 45: 984-987 https://doi.org/10.1136/jnnp.45.11.984
  8. Jakobsen J, Nedergaard M, Aarslew JM, Diemer NH. 1990. Regional brain glucose metabolism and blood flow in st-reptozotocin-induced diabetic rats. Diabetes 39: 437-440 https://doi.org/10.2337/diabetes.39.4.437
  9. Pekiner C, Cullum NA, Hughes JN, Hargreavesm AJ, Mahon J, Casson IF. 1993. Glycation of brain actin in experimental diabetes. J Neurochem 61: 436-442 https://doi.org/10.1111/j.1471-4159.1993.tb02143.x
  10. Kumar JS, Menon VP. 1995. Effect of diabetes on levels of lipid peroxides and glycolipid in rat brain. Metabolism 42: 1435-1439
  11. Candlish JK, Das NP. 1996. Andoxidants in food and chronic degenerative diseases. Biomed Environ Sci 9: 117-123
  12. Bradley PR. 1992. British Herbal Compenstium. British Herbal Medicine Association, Bournemouth. Vol 1, p 73
  13. Bisset NG. 1994. Herbal Drugs and Phtopharmaceuticals. Medpharm, Stuttgart. p 486
  14. Mascolo N, Autore G, Capasso F, Menghini A, Fasulo MP. 1974. Biological screening of Italian medicinal plants for anti-inflammatory activity. Phytother Res 1: 28-31 https://doi.org/10.1002/ptr.2650010107
  15. Cristinf AW, Fiona G, Jenny G. 996. Flavonids. Cinnamic acids and courmarins from the different tissue and medicinal preparations of Taraxacum officinale. Phytochem 42: 121-127 https://doi.org/10.1016/0031-9422(95)00865-9
  16. Report of American Institute of Nutrition. 1977. Ad Hoc committee on standard for nutritional studies. J Nutr 107: 1340-1348
  17. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with folin phenol reagent. J Biol Chem 193: 265-275
  18. Omura T, Sato R. 1964. The carbon monooxide-binding pigment of liver microsomes. I. Evidence for its hemoprotein nature. J Biol Chem 239: 2370-2378
  19. Omura T, Sato R. 1964. The carbon monooxide-binding pigment of liver microsomes. II. Solubilization, purification and properties. J Biol Chem 239: 2379-2385
  20. Imai T, Ito A, Sato R. 1966. Evidence of biochemically different types of vesicles in the hepatic microsomal fraction. J Biochem 60: 417-428 https://doi.org/10.1093/oxfordjournals.jbchem.a128453
  21. Stirpe F, Della CE. 1969. The regulation of rat liver xanthine oxidase: conversion in vitro of the enzyme activity from dehydrogenase (Type D) to oxidase (Type O). J Biol Chem 244: 3855-3863
  22. Marklund S, Marklund G. 1974. Involvement of the superoxide anion radical in the autooxidation of pyrogallol & a convenient assay for superoxide dismutase. Eur J Biochem 47: 469-474 https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  23. Aebi H. 1988. Catalase in vitro. Methods Enz 10: 121-126
  24. Paglia ED, Valentine WN. 1967. Studies on the quantitative and qualitative characterization of erythrocytes glutathione peroxidase. J Lab Clin Med 70: 158-169
  25. Habig WH, Pabist MJ, Jakoby WB. 1974. Glutathione Stransferase: The first enzymatic step in mercapturic acid formation. J Biol Chem 249: 7130-7139
  26. Ellman GL. 1959. Tissue sulfhydryl group. Arch Biochem Biophy 82: 70-77 https://doi.org/10.1016/0003-9861(59)90090-6
  27. Ohkawa H, Ohishi N, Yaki K. 1979. Assay for lipid per-oxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95: 351-358 https://doi.org/10.1016/0003-2697(79)90738-3
  28. Cohn JA, Alvares AP, Kappas A, 1977. On the occurrenceof cytochrome P-450 and arylhydrocarbon hydroxylase activities in rat brain. J Exp Med 145: 1607-1611 https://doi.org/10.1084/jem.145.6.1607
  29. Sasame HA, Ames MM, Nelson SD. 1977. Cytochrome P450 and NADPH cytochrome C reductase in rat brain: Formation of catechols and reactive catechol metabolites. Biochem Biophys Res Commum 78: 919-926 https://doi.org/10.1016/0006-291X(77)90510-1
  30. Schenkman JB, Thummel KE, Favreau LV. 1989. Physio logical and pathophysiological alteration in rat cytochrome P450. Drug Metab Rev 20: 557-584 https://doi.org/10.3109/03602538909103562
  31. Raza H, Ahmed I, John A, Sharma AK. 2000. Modulation of xenobiotic metabolism and oxidative stree in chronic streptozotocin-induced diebetic rats fed with Momordica charantia fruit extract. J Biochem Molecular Toxicol 14: 131-139 https://doi.org/10.1002/(SICI)1099-0461(2000)14:3<131::AID-JBT2>3.0.CO;2-Q
  32. Watts RWE, Watts JEM, Seegmiler JE. 1965. Xanthine oxidase activity in human tissue and its inhibition by allo-purinol. J Lab Clin Med 66: 688-697
  33. Leibovitz BE, Siegel BV. 1980. Aspects of free radical reaction in biological system. Aging J Gerontol 35: 45-56 https://doi.org/10.1093/geronj/35.1.45
  34. Semsei I, Rao G, Richardson A. 1991. Expression of super-oxide dismutase and catalase in rat brain as a function of age. Mech Ageing Devel 58: 13-19 https://doi.org/10.1016/0047-6374(91)90116-H
  35. O'Brien PS, Little C. 1969. Intracellular mechanism for the decomposition of lipid peroxide, II. Decomposition of lipid peroxide by subcellular fraction. Can J Biochem 47: 493-497 https://doi.org/10.1139/o69-077
  36. Brannan TS, Maker HS, Raes TP. 1981. Regional distribution of catalase in the adult rat brain. J Neurochem 86: 307-309
  37. Warner HR. 1994. Superoxide dismutase, aging and degen erative disease. Free Rad Bio Med 17: 249-258 https://doi.org/10.1016/0891-5849(94)90080-9
  38. Kovachich GB, Mishra OP. 1983. The effect of ascorbic acid in malondialdehyde formation, $K^+$, $Na^+$ and water content of brain slices. Exp Brain Res 50: 62-68
  39. Ruth AS, Frederick MR, John JW. III. 2001. Effects of quer-cetin on antioxidant defense in streptozotocin-induced diabetic rats. J Biochem Molecular Toxicol 15: 143-149 https://doi.org/10.1002/jbt.11
  40. Aebi H, Suter H. 1974. Protective function of reduced glu-tathione (GSH) against the effect of prooxidative substances and of irradiation in the red cell. In Glutathione. Flohe LC, Sids HD, Wendel A, eds. p 192-199
  41. Mooradian AD, Dickerson F, Smith TL. 1990. Lipid order and composition of synaptic membranes in experimental diabetes mellitus. Neurochemical Research 15: 981-985 https://doi.org/10.1007/BF00965743
  42. Yagi K. 1991. Role of lipid peroxides in aging and age related disease, In New trends in biological chemistry. Ozawa T, ed. Springer-Verlag, New York. p 207-223

Cited by

  1. Comparison of Antioxidant Activities of Water Extract from Dandelion (Taraxacum officinale) Aerial Parts, Roots, and Their Mixtures vol.44, pp.8, 2015, https://doi.org/10.3746/jkfn.2015.44.8.1157
  2. Platycarya strobilacea S. et Z. Extract Has a High Antioxidant Capacity and Exhibits Hair Growth-promoting Effects in Male C57BL/6 Mice vol.19, pp.3, 2014, https://doi.org/10.3746/pnf.2014.19.3.136
  3. 민들레 분말의 첨가가 파운드 케이크의 품질특성에 미치는 영향 vol.27, pp.3, 2002, https://doi.org/10.14373/jkda.2021.27.3.191