Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Hepatoprotective Effects of Semisulcospira libertina and Garlic on the Liver Damage Induced by Carbon Tetrachloride in Rats

다슬기와 마늘이 사염화탄소로 유발된 랫드의 간손상에 미치는 보호효과

  • 김효정 (경산대학교 한의과대학) ;
  • 김광중 (경상대학교 한의과대학) ;
  • 전태원 ((재)경북테크노파크 경산대학교 한방생명자원특화센터 효능검증원) ;
  • 이은실 ((재)경북테크노파크 경산대학교 한방생명자원특화센터 효능검증원) ;
  • 이영선 ((재)경북테크노파크 경산대학교 한방생명자원특화센터 효능검증원) ;
  • 한옥경 ((재)경북테크노파크 경산대학교 한방생명자원특화센터 효능검증원) ;
  • 박무현 (한국생명과학(주))
  • Published : 2002.06.01
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Abstract

This study was designed to investigate the hepatoprotective effects of Semisulcospira libertina and garlic on the acute hepatotoxicity induced by carbon tetrachloride (CCl$_4$) of rats. Male Sprague-Dawley rats weighing 200∼220g were pretreated with dehydrated powder of Semisulcospira libertina (2.1 g/kg, po; SL) and dehydrated powder mixture of Semisulcospira libertina and garlic (3g/kg, 7:3 ratio, po; SG) once daily for 3 consecutive days, and then given a single dose of CCl$_4$(1g/kg in 5ml/kg corn oil, po) and liver function was determined 24 hrs later. Liver damage was assessed by quantitating activities of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), sorbitol dehydrogenase (SDH) and alkaline phosphatase (ALP) as well as by histopathological examination. Pretreatments with SL and SG significantly decleased CCl$_4$-elevated ALT (48% and 61% respectively), AST (32% and 47%) and SDH (51% and 76%), but had no effect on ALP. SL and SG had revealed hepatoprotective effects against CCl$_4$-induced histopathological changes such as severe necrosis, inflammatory cell infiltration and congestion in the central gene of hepatic lobule. These findings demonstrate that SL and SG may haute the hepatoprotective effect on CCl$_4$-induced liver damage.

다슬기와 마늘 혼합건조분말(3 g/kg b.w. in saline, 7 : 3 ratio, p.o.)의 전처리가 $CCl_4$(1 g/hg b.w in corn oil)의 경구 투여로 유도된 간손상 실험동물모델에 미치는 간보호 효능을 확인하기 위하여 혈청 중 간기능 지표효소 활성도 및 광학현미경적 관찰을 실시하여 다음과 같은 결과를 얻었다. 간기능 지표효소인 혈청 ALT, AST 및 SDH 활성도는 다슬기와 마늘의 전처리로 $CCl_4$단독 투여군에 비해 통계적으로 유의성 있게 감소하였으나 혈청 ALP 활성도는 $CCl_4$ 투여로 증가된 활성을 다슬기와 마늘의 전처리로 감소시키지 못했다. 광학현미경적 관찰에서 $CCl_4$단독 투여군의 경우 인접한 괴사부위와 bridge를 형성한 central necrosis 영역에서 염증 세포의 침윤과 울혈현상이 나타났으나, 다슬기와 마늘의 전처리군에서는 중심영역에서 소핵을 갖는 부푼 형태의 초기 가역적 손상을 입은 간세포가 관찰되었을 뿐 $CCl_4$ 단독 투여군과 같은 염증 세포의 침윤이나 괴사는 관찰되지 않았다. 이상의 실험 결과를 종합해 볼 때, 다슬기와 마늘의 혼합 건조 분말이 간세포의 기능을 유지시킴으로써 사염화탄소 투여에 의해 유발된 간손상을 억제하여 간조직을 보호할 수 있을 것으로 사료된다.

Keywords

References

  1. Davis GM. 1969. A taxonomic study of some species of Semisulcospira in Japan (Mesogastropoda: Pleuroceridae). Malacologia 7: 211-294
  2. 김창민, 신민교, 이경순, 안덕균 1998. 중약대사전. 도서출판 정담, 서울. p 4860-4863
  3. 김세진 1999. 해독보양식품 2선. 도서출판 디딤21, 서울. p 49- 85
  4. Bordia A, Bansal HC. 1973. Letter: Essential oil of garlic in prevention of atherosclerosis. Lancet 2 (7844): 1491-1492
  5. Chang ML, Johnson MA. 1980. Effect of garlic on carbohydrate metabolism and lipid synthesis in rats. J Nutr 110: 931-936 https://doi.org/10.1093/jn/110.5.931
  6. Chi MS, Koh ET, Stewart TJ. 1982. Effects of garlic on lipid metabolism in rats fed cholesterol or lard. J Nutr 112: 241- 248 https://doi.org/10.1093/jn/112.2.241
  7. Fanelli SL, Castro GD, de Toranzo EG, Castro JA. 1998. Mechanisms of the preventive properties of some garlic components in the carbon tetrachloride-promoted oxidative stress. Diallyl sulfide; diallyl disulfide; allyl mercaptan and allyl methyl sulfide. Res Commun Mol Pathol Pharmacol 102: 163-174
  8. Recknagel RO. 1967. Carbon tetrachloride hepatotoxicity. Pharmacol Rev 19: 145-208
  9. Rechnagel RO, Glende EA. 1973. Carbon tetrachloride hepatotoxicity: an example of lethal cleavage. CRC Crit Rev Toxicol 2: 263-297 https://doi.org/10.3109/10408447309082019
  10. Villarruel MC, Diaz Gomez MI, Castro JA. 1975. The nature of the in vitro irreversible binding of carbon tetrachloride to microsomal lipids. Toxicol Appl Pharmacol 33: 106-114 https://doi.org/10.1016/0041-008X(75)90249-5
  11. Glende EA, Pushpendran CK. 1986. Activation of phospholipase A2 by carbon tetrachloride in isolated rat hepatocytes. Biochem Pharmacol 35: 3301-3307 https://doi.org/10.1016/0006-2952(86)90427-2
  12. Long RM, Moore L. 1986. Inhibition of liver endoplasmic reticulum calcium pump by $CCl_4$ and release of a sequestered calcium pool. Biochem Pharmacol 35: 4131-4137 https://doi.org/10.1016/0006-2952(86)90687-8
  13. Plaa GL, Hewitt WR. 1982. Quantative evaluation of indicies of hepatotoxicity. In Toxicology of the Liver. Zakim D, Boyer TD, eds. Raven Press, New York. p 103-120
  14. Recknagel RO. 1983. A new direction in the study of carbon tetrachloride hepatotoxicity. Life Sci 33: 401-408
  15. Reitman S, Frankel S. 1957. A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. Am J Clin Pathol 28: 50-63 https://doi.org/10.1093/ajcp/28.1.50
  16. Gerlach U. 1983. Methods of Enzymatic Analysis. Bergmeyer HU, ed. Academic Press, New York. Vol 3, p 112-117
  17. Bowers GN, McComb RB. 1966. A continuous spectrophotometric method for measuring the activity of serum alkaline phosphatase. Clin Chem 12: 70-89
  18. Robbins LS, Cotran RS. 1979. Pathological basis of disease. WB Saunder Company, London. p 1009
  19. Plaa GL, Hewitt WR. 1989. Detection and evaluation of chemically induced liver injury. In Principles and methods of toxicology. Hayes AW, ed. 2nd ed. Raven Press, New York. p 599-628
  20. Yamamoto H. 1990. Relation of $Ca+^+$ accumulation and lipid peroxidation with $CCl_4$-induced toxicity in the rat liver. Pharmacol Toxicol 66: 213-216 https://doi.org/10.1111/j.1600-0773.1990.tb00735.x
  21. Boyd JW. 1983. The mechanism relating to increases in plasma enzymes and isoenzymes in diseases of animals. Vet Clin Pathol 12: 9-24
  22. Kosrud GO, Grice HC, McLaughlin JM. 1972. Sensitivity of several enzymes in detecting carbon tetrachloride-induced liver damage. Toxicol Appl Pharmacol 22: 474-483 https://doi.org/10.1016/0041-008X(72)90255-4
  23. Perrillo RP, Grifin R, Kecskemeto KD, Launder JJ, Zuckerman GR. 1978. Alcoholic liver disease presenting with marked elevation of serum alkaline phosphatase. Digest Dis 23: 1061-1066 https://doi.org/10.1007/BF01072879
  24. Farombi EO, Nwankwo JO, Emerole GO. 1997. Possible modulatory effect of browned yam flour diet on chemically- induced toxicity in the rat. Food Chem Toxicol 35: 975-979 https://doi.org/10.1016/S0278-6915(97)87266-3
  25. Gole MK, Dasgupta S. 2002. Role of plant metabolites in toxic liver injury. Asia Pac J Clin Nutr 11: 48-50 https://doi.org/10.1046/j.1440-6047.2002.00265.x

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