The Effect of Citric Acid and Sodium Chlorite Mixtures on the Growth of Microorganisms from Broiler Thigh Surface

구연산과 아염소산나트륨 혼합물이 육계 넓적다리 표면의 미생물 증식에 미치는 영향

  • 김태현 (서울산업대학교 식품공학과 및 식품생물공학연구소) ;
  • 이영현 (서울산업대학교 식품공학과 및 식품생물공학연구소)
  • Published : 2002.03.01

Abstract

The effect of citric acid and sodium chlorite(NaClO$_2$) mixtures at 100, 200 and 500 ppd(w/v) on the growth of microorganisms from broiler thigh surface was investigated. Absorbance and aerobic plate counts(APC) of inoculated nutrient broth refrigerated storage at 4∼5$^{\circ}C$ were measured after each additive was added. APC on thigh surface after immersion in each selected solution was enumerated during storage at 4∼5$^{\circ}C$ Absorbance of all citric acid and sodium chlorite mixture(0:100, 25:75, 50:50, 75:25 and 100:0, w/w) except 200 and 500 ppm of 100:0 at Day 0 were lower than the control during storage (p<0.05). And higher concentration of mixture tended to have lower absorbance. APC on thigh surface treated with 500 ppm citric acid and sodium chlorite mixture at 50 : 50 and 75 : 25 ratio were lower than others (p<0.05). Similar antimicrobial activity of additives was found in nutrient broth and on broiler thigh surface.

구연산과 아염소산나트(NaClO$_2$)이 0:100, 25:75, 50:50, 75:25와 100:0(w:w)의 비율로 된 혼합물이 육계 넓적다리 표면에 존재하는 호기성 미생물 증식에 미치는 영향을 조사하였다. 접종된 nutrient broth에 각각의 혼합물을 0, 100, 200과 500 ppm(w/v) 넣어 4~5$^{\circ}C$에서 배양한 후 흡광도와 호기성 미생물 수를 측정하였다. 선택된 혼합물 용액에 넓적다리를 침지한 후 냉장 저장(4~5$^{\circ}C$)하여 호기성 미생물 수를 측정하였다. 구연산과 아염소산나트륨의 혼합물을 첨가한 nutrient broth 흡광도는 100:0의 200 및 500 ppm(w/v)의 처리 직후를 제외하고 저장 기간 동안 모든 비율 및 농도에서 대조구보다 낮은 수치를 나타내었다 (p<0.05). 그리고 첨가물의 농도가 높을수록 흡광도는 낮은 경향을 나타내었다.

Keywords

References

  1. APHA (1992) Compendium of Methods for the Microbiotogical Examination of Food, 3rd ed., American Public Health Association, Washington, DC, USA
  2. Cunningham, F. E. (1979) Shelf-life and quality characteristics of poultry parts dipped in potassium sorbate. J. Food Sci. 44, 863-864 https://doi.org/10.1111/j.1365-2621.1979.tb08522.x
  3. Davidson, P. M., Brekke, C. T. and Branen, A. L. (1981) Antimicrobial activity of butylated hydroxyanisole, tertiary butylhydroquionone and potassium sorbate in combination. J. Food Sci. 46, 314-316 https://doi.org/10.1111/j.1365-2621.1981.tb14596.x
  4. Duncan, D. B. (1955) Multiple range and multiple F tests. Biometrics 11, 1-42 https://doi.org/10.2307/3001478
  5. Harrigan, W. F. and McCance, M. E. (1976) Laboratory Methods in Food and Dairy Microbiology. Academic Press, London
  6. Kotula, A. W. (1966) Variability in microbiological sampling of chickens by the swab method. Poult. Sci. 45, 233-236 https://doi.org/10.3382/ps.0450233
  7. Mermelstein, H. N. (1998) Continuous on-line trials eliminate need for reprocessing poultry. Food Technol. 52, 108-110
  8. Perry, G. A., Lawrence, R. L. and Melnick, D. (1964) Extension of poultry shelf-life by processing with sorbic acid. Food Technol. 18, 891-897
  9. Restaino, L. O., Komatsu, K. K.. and Syracuse, M. J. (1981) Effects of acids on potassium sorbate inhibition of food-related microorganisms in culture media. J. Food Sci. 47, 134-143 https://doi.org/10.1111/j.1365-2621.1982.tb11044.x
  10. Robach, M. C. and Ivey, F. J. (1978) Antimicrobial efficacy of potassium sorbate dip on freshly processed poultry. J. Food Prot. 41, 284-288 https://doi.org/10.4315/0362-028X-41.4.284
  11. SAS/STAT (1988) SAS/STAT User's Guide. Release 6.03, SAS Institute Inc., Cray, NC, USA
  12. Yi, Y. H. and Kim, T. H. (1999) The effects of quaternary ammonium salt, trisodium phosphate, sodium hypochlorite and calcium hypochlorite on the growth of microorganisms from broiler thigh surface. Journal of Korean Society for Food Science of Animal Resources, 19, 160-168
  13. Yi, Y. H., Tanteeratarm, K. and Chen. T. C. (1995) The Growth-interfering effects of G+cocci isolates on poultry spoilage microorganisms. Foods and Biotech. 4, 273-279
  14. Yoo, I. J. (1990) Effects of potassium sorbate or ascorbic acid dip on microbial and sensory quality of refrigerated chicken. K. J. Poult. Sci. 17, 193-202