Abstract
The economical producing method of casein phosphopeptide (CPP) and the physicochemical properties related to the solubilization of calcium were studied. Firstly, The compositions of the purified CPP-III were 13.1% of nitrogen, 2.3∼2.4% of phosphate and the ratio of N/P was 5.4∼5.6. In consideration of economic aspects, the preparation method of the CPP- I and II which were lower purity than the CPP-III was established. The physico-chemical property of the CPP was compared with the enzymically dephosphorylated CPP. CPP and polyglutamate effectively inhibited the formation of insoluble calcium phosphates at physiological pH.
본 연구는 생리활성 casein phosphopeptide의 경제적인 생산방법과 CPP의 칼슘 가용화 능력을 검정하기 위하여 수행되었다. 먼저 정제된 CPP-III는 N 13.1%, P 2.3∼2.4%, N/P비 5.4∼5.6 정도로 CPP 순도가 매우 높은 것으로 나타났다. 경제적인 면을 고려하여 CPP-III보다는 순도가 떨어지는 CPP-I, CPP-II의 제조방법을 확립하였다. 한편 CPP의 칼슘가용화 능력을 생리적 pH 조건하에서 검정한 결과 CPP와 polygutamic acid는 Ca 침전 형성 저지효과가 현저하였으나, DPP는 효과가 없었다.
