Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Distribution of Microflora in Powdered Raw Grains and Vegetables and Improvement of Hygienic Quality by Gamma Irradiation

유통 생식제품의 미생물 분포 및 감마선 조사를 이용한 위생화

  • Kim, Dong-Ho (Team for Radiation Food Science and Biotechnology, Korea Atomic Energy Research Institute) ;
  • Song, Hyun-Pa (Team for Radiation Food Science and Biotechnology, Korea Atomic Energy Research Institute) ;
  • Yook, Hong-Sun (Dept. of Food Nutrition, Chungnam National University) ;
  • Chung, Young-Jin (Dept. of Food Nutrition, Chungnam National University) ;
  • Kim, Yeung-Ji (Dept. of Food Science and Nutrition, Yeungnam College of Science and Technology) ;
  • Byun, Myung-Woo (Team for Radiation Food Science and Biotechnology, Korea Atomic Energy Research Institute)
  • 김동호 (한국원자력연구소 방사선식품·생명공학연구팀) ;
  • 송현파 (한국원자력연구소 방사선식품·생명공학연구팀) ;
  • 육홍선 (충남대학교 식품영양학과) ;
  • 정영진 (충남대학교 식품영양학과) ;
  • 김영지 (영남이공대학 식품영양과) ;
  • 변명우 (한국원자력연구소 방사선식품·생명공학연구팀)
  • Published : 2002.08.01
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Abstract

Improvement of hygienic quality of powdered raw grains and vegetables by gamma irradiation was investigated. Five products of powdered raw grains and vegetables were collected in a local market and analysed. The total viable cell counts of Bacillus were 10$^4$~10$^{7}$ cfu/g, filamentous fungi l0$^2$~10$^3$cfu/g, coliform 10$^1$~10$^4$cfu/g, enteric bacteria on SS agar plate 10$^1$~10$^3$cfu/g. Coliform, enteric bacteria on SS agar plate and filamentous fungi were eliminated by 3 kGy of gamma irradiation. The D values of coliform, enteric bacteria on SS agar plate, Bacillus, and filamentous fungi were 0.68~0.80 kGy, 0.59~0.74 kGy, 1.84~2.18 kGy and 0.36~0.57 kGy, respectively. It was considered that optimal irradiation dose for radappertization was about 25 kGy, while 3~5 kGy of gamma irradiation was effective for radicidation.

유통 생씩 5종의 수분 함량 4.22~7.18%의 범위였으며 수분활성은 0.15~0.26의 분포를 보여 미생물에 의한 변패보다는 낮은 수분활성에서도 생존해 있는 병원성 미생물의 제거가 생식의 중요한 품질관리 요소일 것으로 판단된다. 생식의 미생물분포는 Bacillus group $10^4$~$10^{7}$ cfu/g, 사상균류 $10^2$~$10^3$cfu/g, coliform group $10^1$~ $10^4$cfu/g, SS agar plate 분리 enteric bacteria group $10^1$~$10^3$cfu/g의 수준으로 특히 병원성 미생물의 오염가능성이 컸다. 감마선 조사 결과 coliform group, SS agar plate 분리 enteric bacteria group, 그리고 사상균류는 3 kGy의 조사선량에서 완전살균 수준으로 제거되었다. 생식 분포 미생물의 D값은 coliform group은 0.68~0.80 kGy, SS agar plate 분리 enteric bacteria group은 0.59~0.74 kGy, Bacillus group은 1.84~2.18 kGy, 사상균류는 0.36~0.57 kGy의 범위를 나타내었다. 생식제품의 위생화를 목적으로 할 경우 감마선조사 선량은 생식제품에 분포하는 coliform group과 SS agar plate 분리 enteric bacteria group 미생물의 사멸기준인 3~5 kGy의 수준으로 설정하는 것이 바람직할 것으로 사료된다.

Keywords

References

  1. Kwak NS, Shin HH. 2000. Control of health food. Food Science and Industry. 33(4): 43-51.
  2. Mazza G. 1998. Functional foods. 1st ed. Technomic Publishing Co., Lancaster. p 1-234.
  3. Byun MW. 1997. Application and aspect of irradiation technology in food industry. Food Sci and Industry 30(1): 89-100.
  4. Kilcast D. 1996. Food irradiation: Current problems and future potential. International Biodeterioration & Biodegradation p 279-296.
  5. AOAC. 1980. Official methods of analysis. 13th ed. Association of official analytical chemists, Washington DC.
  6. Difco Laboratories. 1984. Difco manual. 10th ed. Detroit, Michigan, USA.
  7. FAO/IAEA/WHO Study Group. 1999. High-dose irradiation: Wholesomeness of food irradiated with doses above 10 kGy. In WHO technical report series 890. World Health Organization, Geneva. p 54.
  8. Ray B. 2001. Fundamental food microbiology. 2nd ed. CRC Press, New York. p 455-462.
  9. Ray B. 2001. Fundamental food microbiology. 2nd ed. CRC Press, New York. p 43-53.
  10. Maxcy RB, Tiwari NP. 1973. Irradiation of meats for public health protection. In Radiation preservation of food. International Atomic Energy Agency, Vienna. p 491-504
  11. FAO/IAEA/WHO Study Group. 1999. High-dose irradiation: Wholesomeness of food irradiated with doses above 10 kGy. In WHO technical report series 890. World Health Organization, Geneva. p 49-77.
  12. Anellis A, Berkowitz D, Kemper D. 1973. Comparative resistance of non-sporogenic bacteria to low-temperature gamma radiation. Applied Microbiology 25: 517-523.
  13. Davies R. 1976. The inactivation of vegetative bacterial cells by ionizing radiation. In Inhibition and inactivation of vegetative microbes. Skinner FA, Hugo WG, eds. Academic Press, London. p 279-304.
  14. Ma K, Maxcy RB. 1981. Factors influencing radiation resistance of vegetative bacteria and spores associated with radappertization of meat. J Food Sci 46: 612-616. https://doi.org/10.1111/j.1365-2621.1981.tb04923.x
  15. Grant IR, Patterson MF. 1992. Sensitivity of foodborne pathogens to irradiation in the components of a chilled ready meal. Food Microbiology 9: 95-103 https://doi.org/10.1016/0740-0020(92)80017-X
  16. Briggs A. 1966. The resistance of spores of the genus Bacillus to phenol, heat and radiation. J Appl Bacteriol 29: 490-504. https://doi.org/10.1111/j.1365-2672.1966.tb03500.x
  17. Haurnulv BG, Snygg BG. 1973. Radiation resistance of spores of Bacillus subtilis and B. stearothermophilus at various water activities. J Appl Bacteriol 36: 677-682. https://doi.org/10.1111/j.1365-2672.1973.tb04152.x
  18. Blank G, Corrigan D. 1995. Comparison of resistance of fungal spores to gamma and electron beam radiation. International Journal of Food Microbiology 26: 269-277. https://doi.org/10.1016/0168-1605(94)00129-T
  19. Ito H, Iizuka H, Sato T. 1973. Identification of osmiophillic Aspergillus isolated from rice and their radiosensitivity. Agric Biol Chem 37: 789-798. https://doi.org/10.1271/bbb1961.37.789

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