Optimization for Hot Water Extraction Process of Cordyceps militaris using Response Surface Methodology

반응표면분석법에 의한 동충하초 열수추출공정의 최적화

  • Published : 2002.06.01

Abstract

This study was conducted to optimize hot water extraction process of cordyceps militaris for development beverage. Optimal condition for hot water extraction was investigated with changes in extraction concentration and temperature by response surface methodology. The content of soluble solid was effected with concentration. The efficiency of extraction for turbidity, total sugar, reducing sugar and protein increased with low concentration. The response variables had significant with concentration and the established polynomial model was suitable (p>0.05) model by lack-of-fit analysis. Optimal extraction conditions as the limited renditions of 2.0∼2.4% extractable solids, 1.5∼2.0% brix, l14∼120 $\mu\textrm{g}$/g total sugar, 10.5∼11.0 $\mu\textrm{g}$/g reducing sugar and 110∼l15 $\mu\textrm{g}$/g protein were 95∼100$\^{C}$ and 4.0∼4.1% of concentration.

동충하초를 음료로 개발하기 위한 전단계로 열수추출공정을 반응표면분석법으로 최적화하였다. 추출시간에 따라서는 큰 변화가 없어 추출온도와 농도를 요인변수로 하여 실험을 수행하였다. 열수추출액의 당도(brix)와 고형분 함량은 농도에 따라서 큰 영향을 받는 것으로 나타났다. 추출액의 탁도와 총당, 환원당 및 단백질 함량은 낮은 농도에서 추출수율이 좋은 것으로 나타났다. 각 반응변수 모두 농도에 대하여는 유의성이 있는 것으로 나타났으며 수립된 이차 회귀식에 대한 적합성 결여분석 결과 수립된 반응표면 모형이 통계적으로 유의하였다. 동충하초 열수추출에 있어 고형분함량은 2.0~2.4%, brix 1.5~l.8, 총당 l14~120 $\mu\textrm{g}$/g, 환원당 10.5~11.0 $\mu\textrm{g}$/g 그리고 단백질함량 110~l15 $\mu\textrm{g}$/g인 영역을 만족하는 조건은 추출온도 95~10$0^{\circ}C$, 농도 4.0~4.1%로 결정할 수 있었다.

Keywords

References

  1. 한대석, 송효남, 김상희 (1998) 동충하초 새로운 기능성 식품소재, 식품기술 11, 107-116
  2. Han D.S., Song, A.N. and Kim Y.E. (1999) State, problem and research direction of Cordyceps sp. Food Science and Industry, 32, 67-82
  3. Shim, J.Y., Lee Y.S., Lim, S.S. Shin, K.H. Hyun, J.E., Kim, S.Y. and Lee, E.B. (2000) Pharmacological activities of Paecilomyces japonica, a new type Cordyseps sp. Kor. J. Pharmacogn., 31, 163-168
  4. Lee, G.D., Lee, J.E. and Kwon, J.H. (2000) Application of response surface methodology in food chemistry, Food and Industry, 33, 33-45
  5. Miller, G.L. (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31, 426-428 https://doi.org/10.1021/ac60147a030
  6. Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A. and Smith, F. (1956) Colorimetric method for determination of sugar and related substance. Anal. Chem., 28, 350-352 https://doi.org/10.1021/ac60111a017
  7. Lowry, O.H. and Rosebrough, N.J. (1951) Protein measurement with the folin-phenol reagents. J. Biol. Chem., 193, 256-265
  8. Kang, K.C., Park, J.H. Baek, S.B., Jhin, H.S. and Rhee, K.S. (1992) Optimization of bebrage preparation from Schizandra chinensis baillon by response surface methodology, Korean J. Food Sci, Technol., 24, 74-81
  9. Kim, S.D., Ku, Y.S, Lee, I.Z, Park, I.K. and Youn K.S. (2001) Optimization for hot water extraction condition of Liriope spicata tuber using response surface methodology, Korean J. Postharvest Sci. Technol., 8, 157-163