Antioxidative and Free Radical Scavenging Activity of Water Extract From Dandelion (Taruaxacum officinale)

민들레 물추출물의 항산화 및 자유라디칼 소거활성

  • 강미정 (영남대학교 식품영양학과) ;
  • 신승렬 (경산대학교 생명자원공학부) ;
  • 김광수 (영남대학교 식품영양학과)
  • Published : 2002.06.01

Abstract

The antioxidative and free radical scavenging activity of water extracts of dandelion were investigated. Antioxidative and radical scavenging activity were assessed by means of different tests; inhibition of peroxidation on linoleic acid model system, scavenging DPPH radical, scavenging of hydroxyl radical by chemiluminescence assay, scavenging of superoxide anion radical by EPR spectroscopy and scavenging of hydrogen peroxide. The leaf extract showed strong antioxidant activity in linoleic acid system. The antioxidant activity of water extracts of dandelion increased with increasing concentrations of extracts. The scavenging activity of the dandelion extracts, on inhibition of the DPPH radical, was related to the reaction time. Hydroxyl radical were generated by lenten reaction and dandelion extract was found to scavenge OH˙in a concentration-dependent manner. The water extract of leaf had effective scavenging activities on hydrogen peroxide and superoxide anion radical. From the these data, it is evident that water extract of dandelion leaf is an effective scavenger for OH˙, O$_2$¨, DPPH˙, hydrogen peroxide. And, the antioxidative effect observed is believed to be partly due to this radical scavenger activity.

민들레 잎과 뿌리로부터 물추출물을 제조하여 항산화 활성 및 라디칼 소거활성을 분석하기 위하여 linoleic acid에 대한 과산화물 생성저해율, DPPH radical에 대한 소거활성, chemiluminescence를 이용한 hydroxyl radical에 대한 소거활성, EPR을 이용한 superoxide anion radical에 대한 소거활성및 hydrogen peroxide에 대한 소거활성을 측정하였다. 그 결과, 민들레 잎의 물추출물이 뿌리의 물추출물 보다 지방산에 대한 과산화물 생성 저해율이 높았고, DPPH radical, hydroxyl radical, superoxide anion radical 및 hydrogen peroxide에 대한 소거활성 역시 매우 높은 것으로 나타났다.

Keywords

References

  1. Halliwell, B. and Gutteridge, J.M.C. (1999) Free radicals in biology and medicine. Third ed., Oxford University Press, New York
  2. Halliwell, B. and Aruoma, O.I. (1991) DNA damamage by oxygen-derived species. FEBS Letters, 281, 9-19 https://doi.org/10.1016/0014-5793(91)80347-6
  3. Poli, G., Albano, E. and Dianzani, M.U. (1993) Free radicals-From basic science to medicine. Molecular and Cell Biology Updates, Birkh?user Verlag
  4. Kim, S.J. (1994) Effect of natural antioxidants on reactive oxygen species. Ph.D. Thesis, Korea Advanced Institute of Science and Technology
  5. Punchard, N.A. and Kelly, F.J. (1996) Free radicals-A Practical approach. Oxford University Press, New York
  6. Park, S.N. (1997) Skin aging and antioxidants. Journal of the Society of Cosmetic Chemists of Korea, 23, 75-132
  7. Lee, J.M. (1997) Protective effect of Ganoderma lucidum and Panax ginseng, C.A. Meyer on oxidative damage. M.S. Thesis, Seoul National University
  8. 이양자, 이종호, 박태선(1998) 항산화영양소와 건강. 연세대학교 식품영양과학연구, 1-58
  9. Chung, S.Y. (1997) Antioxidant nutrients and related biological activities of green yellow vegetable juices. Ph.D. Thesis, Yonsei University
  10. Hong, S.H. (1998) : Antioxidant activity of melatonin and melatonin related indoleamines. M.S. Thesis, Yonsei University
  11. Cho, T.Y. (1999) : Free radical scavenging activity of tryptophan metabolites. M.S. Thesis, Yonsei University
  12. 김현구 (1996) 영양적 항산화제로서 비타민 E, 비타민 C 및 카로테노이드. 식품기술, 9(3), 190-195
  13. Namiki M.o (1990) Antioxidants/antimutagens in food. Critical Reviews in Food Science and Nutrition, 29, 273-300 https://doi.org/10.1080/10408399009527528
  14. 신동화(1997) 천연 항산화제의 연구동향과 방향. 식품과학과 산업, 30, 14-21
  15. 이정수, 최홍식(1994) Free radical scavenger로서의 plant phenolics의 특성과 항산화 활성. 생명과학, 4, 11-18
  16. Kim, I.W. (1999) Isolation and identification of antioxidative components from ethanol extract of Rhus verniciflua STOKES and application to different oil system. Ph.D. Thesis, Chonbuk National University
  17. Shahidi, F., Janitha, P.K. and Wanasundara, P.D. (1992) : Phenolic antioxidants. Critical Reviews in Food Science and Nutrition, 32, 67-103 https://doi.org/10.1080/10408399209527581
  18. Yoshiki, Y., Okubo, K., Onuma, M. and Igarashi, K. (1995) Chemiluminescence of benzoic and cinnamic acids, and flavonoids in the presence of aldehyde and hydrogen peroxide of hydroxyl radical by fenton reaction. Phytochemistry, 39, 225-229 https://doi.org/10.1016/0031-9422(94)00896-2
  19. Salah, N., Miller, N.J., Paganga, G., Tijburg, L., Paul-Bolwell, G. and Rice-Evans, C. (1995) Polyphenolic flavanols as scavengers of aqueous phase radicals and as chain-breaking antioxidants. Archives of Biochemistry and Biophysics, 322, 339-346 https://doi.org/10.1006/abbi.1995.1473
  20. Kim, N.M., Sung, H.S. and Kim W.J. (1993) Effect of solvents and some extaction conditions on antioxidant activity in cinnamon extracts. Korean J. Food Sci. Technol., 25, 204-209
  21. Kye I.S. (1999) Studies on scavenging activity of peroxynitrite and reactive oxygen species of green tea polyphenols. Ph.D. Thesis, Pusan National University
  22. Yokozawa, T., Dong, E., Nakagawa, T., Kashiwagi, H., Nakagawa, H., Takeuchi, S. and Chung, H.Y. (1998) In vitro and in vivo studies on the radical-scavenging activity of tea. J. Agric. Food. Chem., 46, 2143-2150 https://doi.org/10.1021/jf970985c
  23. Ruch, R.J., Cheng, S.J. and Klaunig, J.E. (1989) Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from chines green tea. Carcinogenesis, 10, 1003-1008 https://doi.org/10.1093/carcin/10.6.1003
  24. 기능성식품소재연구회(1996) 식품 중의 생체기능조절물질연구법. 송현문화사
  25. Duh, P.D., Tu, Y.Y. and Yen, G.C. (1999) Antioxidant activity of water extract of Harng Jyur(Chrysanthemum morifolium Ramat). Lebensm.-Wiss.u.-Technol., 32, 269-277
  26. Brand-Williams, W., Cuvelier, M.E. and Berset, C. (1995) Use of a free radical method to evaluate antioxidant activity. Lebensm.-Wiss.u.-Technol., 28, 25-30 https://doi.org/10.1016/S0023-6438(95)80008-5
  27. Bondet, V., Brand-Williams, W. and Berset, C. (1997) Kinetics and mechanism of antioxidant activity using the DPPH˙free radical method. Lebensm.-Wiss.u.-Technol., 30, 609-615 https://doi.org/10.1006/fstl.1997.0240
  28. Hirayama, O., Takagi, M., Hukumoto, K. and Katoh, S. (1997) Evaluation of antioxidant activity by chemiluminescence. Analytical Biochemistry, 247, 237-241 https://doi.org/10.1006/abio.1997.2053
  29. Hirayama, O. and Yida, M. (1997) Evaluation of hydroxyl radical scavenging ability by chemiluminescence. Analytical Biochemistry, 251, 297-299 https://doi.org/10.1006/abio.1997.2282
  30. Ruch, R.J., Cheng, S.J. and Klaunig, J.E. (1989) Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from chines green tea. Carcinogenesis, 10, 1003-1008 https://doi.org/10.1093/carcin/10.6.1003
  31. Duh, P.D. and Yen, G.C. (1997) Antioxidative activity of three herbal water extracts. Food Chemistry, 60, 639-645 https://doi.org/10.1016/S0308-8146(97)00049-6
  32. Kim, B.Y. (1996) Scavenging of hydroxyl radicals by chlorophyllin. M.S. Thesis, The Korea Advanced Institute of Science and Technology
  33. Kang, K.H. (1998) Antioxidant and pro-oxidant activity of plant extracts. M.S. Thesis, Seoul National University
  34. Atanasiu, R.L., Stea, D., Mateescu, M.A., Vergely, C., Dalloz, F., Briot, F., Maupoil, V., Nadeau, R. and Rochette, L. (1998) Direct evidence of caeruloplasmin antioxidant properties. Molecular and Cellular Biochemistry, 189, 127-135 https://doi.org/10.1023/A:1006945713860