Effect of Ethyl Acetate Extract of the Dried Prunus mume on the Growth of Staphylococcus aureus

오매의 에틸아세테이트 추출물이 Staphylococcus aureus의 증식에 미치는 영향

  • 양미옥 (계명대학교 식품영양학과) ;
  • 배지현 (계명대학교 식품영양학과)
  • Published : 2002.06.01

Abstract

The dried Prunus mume, an alkaline food abundant in organic acids (citric acid, malic acid and tartaric acid), has been largely used in both folklore remedies and Chinese herbal medicine for a long time. This study was performed to investigate the antimicrobial activity of the dried Prunus mume. The fractionation of the methanol extracts from Prunus mume was conducted using petroleum ether, chloroform, ethyl acetate and butanol, respectively. The antimicrobial activity of the Prunus mume extracts was then determined against food-borne pathogens using a paper disc method. The ethyl acetate extract showed the strongest antimicrobial activity against the eigth food-born pathogens used in this present study. Diaion HP 20 column chromatography was performed to remove some sugars that might inhibit the antimicrobial activity of Prunus mume. The strongest antimicrobial activity of ethyl acetate fraction of Prunus mume was shown against Staphylococus aureus. The growth inhibition curve was determined using ethyl acetate extracts of Prunus mume against Staphylococus aureus, which showed the growth inhibition up to 72 hours at 1,000 ppm concentration.

Keywords

References

  1. Food additives dictionary Ministry of Health and Welfare
  2. J. Food Protect v.52 Antimicrobial activity of some Egyptian spice essential oils Farag,R.S.;Daw,Z.Y.;Hewedi,F.M.
  3. Appl. Microbiol. v.18 Isolation of bacterial inhibition from green olives Fleming,H.P.;Walter,W.M.;Etchells,J.R.
  4. J. Food Sci. v.54 Influence of some spice essential oil on Aspergillus parasiticus growth and production of aflatoxin in a synthetic medium Farag,R.S.;Daw,Z.Y.;Abo-raya,S.H.
  5. Phytochemistry Research v.4 Antimicrobial activity of Juglone Clark,A.M.;Jurgen,T.M.;Hufford,C.D.
  6. Food Technol. v.43 Antimicrobials occurring naturally in foods Beuchat,L.R.;Golden,D.A.
  7. Phytochemistry v.25 no.5 Carotene sesquiterpens from Ferura linkii Diaz,J.G.;Fraga,B.M.;Gonzalz,A.G.;Gonzalez,P.;Hernandez,M.G.;Perales,A.
  8. Antimicrobials in Foods Naturally occurring and miscellaneous food antimicrobials Davidson,P.M.;Post,L.S.;Branen,A.L.(ed.);Davidaon,P.M.(ed.)
  9. Masters Thesis of Yonsei University Studies on the changes of physical and chemical compositions in ripening of Prunus mume Choi,C.W.
  10. Masters Thesis of Kyungnam University Effect of Maesil extracts injection on blood lactate density and serum lipid components Youn,M.S.
  11. Masters Thesis of Chosun University Effect of Prunus mume extracts on experimentally alloxan induced diabetesin rabbits Ko,E.Y.
  12. J. Korean Soc. Food Nutr. v.19 no.1 Effect of prunus mume extract on gastric secretion in rats and carbon tetrachloride induced liver damage of rabbits Sheo,H.J.;Lee,M.Y.;Chung,D.L.
  13. Traditional drugs of the east Kim,J.H.
  14. J. East Asian of Dietry Life v.9 no.2 Effect of Prunus mume extract beverages on the growth of food-borne pathogens Bae,J.H.;Kim,G.J.
  15. Pharm. Acta. Hev. v.67 no.5-6 Antimicrobial antibiotics Didry,N.;Dubreuil,L.;Pinkas,M.
  16. J. Food Microbiol. v.10 Inhibitiory effects of anethole and eugenol on the growth and toxin production of Aspergillus parasiticus. International Karapinar,M.
  17. J. Nut. Product. v.55 no.10 Antibacterial activity of totarol and its potentiation Kubo,S.;Muroi,H.;Himejima,M.
  18. J. Nat. Products v.50 no.6 Tradition medical plants of thailand, Ⅳ.10-hydrxy-11- methoxydarcaenone and 7,10-dihydroxy-11-eth-oxydracaenone from dracaena loureiri Meksuriyen,D.;Cordell,G.A.
  19. Korean J. Postharvest Sci. Technol. v.6 no.2 Antimicrobial activities of extracts of Perilla frutescens briton var. acuta kudo on food spoliage or foodborne disease microorganisms Lee,K.S.;Lee,J.C.;Han,K.H.;Oh,M.J.
  20. Kor. J. Food Sci. Technol. v.24 no.6 Studies on antimicrobial substances of Ganoderma lucidum Chung,D.O.;Jung,J.H.
  21. Korean J. Soc. Food. Nutr. v.23 no.6 Screening of antimicrobial activity of leaf Mustard extracts Kang,S.K.;Sung,N.K.;Kim,Y.D.;Shin,S.C.
  22. Korean J. Food Sci. Technol. v.26 no.5 Antimicrobial effect of ethanol extracts of Sinomenium acutum(Thunb) rdhd,et wils and Glycyrrhiza glabar L. var, glandnlifera fegel et zucc on Listeria monocytogenes Shin,D.H.;Han,J.S.
  23. Kor. J. Pharmacogn v.18 Antimicrobial effect of skullcap plant Lee,I.L.;Park,H.S.