Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Detection of Irradiated Astragalus membranaeus Bunge and Havenia duzcis Thumb Using DNA Comet Assay

  • Yi, Jin-Hee (Department of Food Science and Technology, Chungnam National University) ;
  • Song, Kyung-Bin (Department of Food Science and Technology, Chungnam National University)
  • Published : 2002.09.01
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Abstract

Ionizing radiation can be used to sanitize herbs contaminated by various microorganisms. However, health concerns related to irradiation damage to complex molecules in plants necessitate that methods be developed to monitor such damage. To elucidate DNA damage of herbs caused by irradiation, the DNA comet assay was used for Astragalus membranaceus Bunge and Havenia dulcis Thumb, irradiated at 1, 5, 7, and 10 kGy. With increasing irradiation doses, the tails of comets became longer with average tail length increasing from 17 (non-irradiated) to 124 (10 kGy) $\mu$m in Astragalus membranaceus Bunge. Above 7 kGy, some of the tails were separated from the heads of comets. Distribution patterns of the tail length of In comets selected randomly in the irradiated herbs were analyzed to quantify the DNA damage. These results clearly suggest that the DNA comet assay is an effective and inexpensive tool for the detection of irradiation damage to DNA in herbs.

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References

  1. Delince H. 1998. Detection of food treated with ionizing radiation. Trends Food Sci Technol 9: 73-82 https://doi.org/10.1016/S0924-2244(98)00002-8
  2. Sharma A, Gautam S, Thomas P. 1998. Improved bacterial turbidimetric method for detection of irradiated spices. J Agric Food Chem 46: 5110-5112 https://doi.org/10.1021/jf980325n
  3. Ciesla K, Svensson E, Eliasson AC. 1999. Preliminary studies using differential scanning calorimetry of radiation-induced transformations in starch and flour. J Therm Anal Calorim 56: 1197-1202 https://doi.org/10.1023/A:1010173516020
  4. Lewandowicz G, Jankowski T, Formal J. 2000. Effect of microwave radiation on physicochemical properties and structure of cereal starches. Carbohydr Polym 42: 193-199 https://doi.org/10.1016/S0144-8617(99)00155-1
  5. Delincée H. 1993. Control of irradiated foods: Recent developments in analytical methods. Radiat Phys Chem 42: 351- 357 https://doi.org/10.1016/0969-806X(93)90265-V
  6. Östling O, Johanson KT. 1984. Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochem Biophys Res Commun 123: 291-298 https://doi.org/10.1016/0006-291X(84)90411-X
  7. Cerda H. 1998. Detection of irradiated fresh chicken, pork and fish using the DNA comet assay. Lebens Wiss Technol 31: 89-92 https://doi.org/10.1006/fstl.1997.0304
  8. Cerda H, Delince H, Haine H, Rupp H. 1997. The DNA “Comet assay” as a rapid screening technique to control irradiated food. Mutation Res 375: 167-181 https://doi.org/10.1016/S0027-5107(97)00012-2
  9. Klaude M, Eriksson S, Nygren J, Ahnstrm G. 1996. The com-et assay: mechanisms and technical considerations. Mutation Res 363: 89-96 https://doi.org/10.1016/0921-8777(95)00063-1
  10. Cerda H, Koppen G. 1998. DNA degradation in chilled fresh chicken studied with the neutral comet assay. Z Lebensm Unters Forsch 207: 22-25 https://doi.org/10.1007/s002170050289
  11. Olive PL. 1989. Cell proliferation as a requirement for development of the contact effect in Chinese hamster V79 spheroides. Radiant Res 117: 79-92 https://doi.org/10.2307/3577279
  12. Kent CRH, Eady JJ, Ross GM, Steel GG. 1995. The comet moment as a measure of DNA damage in the comet assay. Int J Radiat Biol 67: 655-660 https://doi.org/10.1080/09553009514550771