Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Cloning of Genomic DNAs of Trametes versicolor Acting as Autonomously Replicating Sequences in Saccharomyces cerevisiae

  • Sora An (Division of Life Science, College of Natural Science, Kangwon National University) ;
  • Park, Kyoung-Phil (Division of Life Science, College of Natural Science, Kangwon National University) ;
  • Park, Hyoung-Tae (Division of Life Science, College of Natural Science, Kangwon National University) ;
  • Kim, Kyu-Joong (Department of Biology, Kangnung National University) ;
  • Kim, Kyunghoon (Division of Life Science, College of Natural Science, Kangwon National University)
  • Published : 2002.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

A genomic DNA library of the fungus Trametes versicolor was constructed in a yeast integration vector which contains the URA3 gene of the budding yeast Saccharomyces cerevisiae and the gene responsible for hygromycin B resistance, and fragments acting as autonomously replicating sequences (ARSes) in the budding yeast were identified from the genomic DNA library. Sixteen recombinant plasmids from the library transformed the budding yeast Saccharomyces cerevisiae to Ura+ at high frequencies. They were maintained stably under selective conditions, but were gradually lost from yeast cells at different rates under nonselective conditions, indicating that they contain eukaryotic origins of DNA replication and exist as extrachromosomal plasmids. Base sequences of four ARS DNAs among the 16 cloned fragments revealed that all or the four contain at least one 11 bp ［(A/T)TTTA(T/C)(A/G)TTT(A/T)］consensus sequence of the budding yeast ARS.

Keywords

References

  1. Handbook of Applied Mycology, Fungal Biotechnology v.4 Fungal degradation of lignin biotechnological applications Boominathan, K.;C.A. Reddy;D.K. Arora(eds.);R.P. Elander(eds.);K.G. Mukerji(eds.)
  2. Cold Spring Harbor Symp. Quant. Biol v.47 Localization and sequence analysis of yeast origins of DNA replication Broach, J.R.;Y.-Y. Li;J. Feldman;M. Jayaram;J. Abraham;K.A. Nasmyth;J.B. Hicks
  3. Science v.228 Oxidation of persistent environmental pollutants by a white rot fungus Bumpus, J.A.;M. Tien;D. Wright;S.D. Aust.
  4. Appl. Microbiol. Biotechnol v.52 Characterization of a gene encoding Trametes versicolor laccase A and improved heterologous expression in Saccharomyces cerevisiae by decreased cultivation temperature Cassland, P.;L. Jonsson
  5. Appl. Environ. Microbiol v.65 Cloning and characterization of a cDNA encoding a novel extracellular peroxidase from Trametes versicolor Collins, P.;M. O'Brien;A. Dobson.
  6. Enzyme Microbiol. Technol v.7 Mineralization of polychlorinated biphenyls by Phanerochaete chrysosporium Eaton, D.C.
  7. Microbiol. Rev. v.57 Molecular biology of the lignin-degrading basidiomycete Phanerochate chrysosporium Gold, M.H.;M. Alic.
  8. J. Basic Microbiol v.38 Degradation of 2-chlorophenol and formation of 2-chloro-1,4-benzoquinone by mycelia and cell-free crude liquids of Trametes versicolor in relation to extracellular laccase activity Grey, R.;C. Hofer;C. Schlosser
  9. Appl. Environ. Microbiol v.58 Oxidative degradation of phenanthrene by the ligninolytic fungus Phanerochaete chrysosporium Hammel, K.E.;W.Z. Gai;B. Green;M.A. Moen
  10. Proc. Natl. Acad. Sci. USA v.76 High-frequency transformation of yeast plasmids containing the cloned yeast ARG4 gene Hsiao, C.L.;J. Carbon.
  11. Gene v.170 A cluster of genes encoding major isozymes of lignin peroxidase and manganese peroxidase from the white-rot fungus Trametes versicolor Johansson, T.;P. Nyman
  12. Cell v.37 Structural requirements for the function of a yeast chromosomal replicator Kearsey, S.
  13. FEMS Microbiol. Lett v.209 Transformation of the medicinal basidiomycete Trametes versicolor to hygromycin B resistance by restriction enzyme mediated integration Kim, K.;Y. Leem;K. Kim;K. Kim;H. Choi
  14. Annu. Rev. Microbiol v.41 Enzymatic combustion; the microbial degradation of lignin Kirk, T.K.;R.L. Farrell.
  15. FEMS Microbiol. Lett v.172 Transformation and laccase mutant isolation in Coprinus congregatus by restriction enzyme-mediated integration Leem, Y.;S. Kim;I.Ross;H. Choi.
  16. Gene v.196 Cloning and sequence analysis of two laccase complementary DNAs from the ligninolytic basidiomycete Trametes versicolor Ong, E.;W. Pollck;M. Smith.
  17. Gene v.56 Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli Punt, P.J.;R.P. Oliver;M.A. Dingemanse;P.H. Pouwels;C. van den Hondel.
  18. FEMS Microbiol. Rev v.13 Physiology and molecular biology of the lignin peroxidases of Phanerochaete chrysosporium Reddy, C.A.;T.M. D'Souza.
  19. Genetics v.122 A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae Sikorski, R.S.;P. Hieter
  20. Nature v.282 Isolation and characterization of a yeast chromosomal replicator Stinchcomb, K.T.;K. Struhl;R.W. Davis.
  21. Mol. Cell. Biol. v.10 Mutational analysis of the consensus sequence of a replication origin from yeast chromosome III van Houten, J.V.;C.S. Newlon.