A Characteristics of Phagocytic Plaque on Staphylococcus aureus Layer Formed by Leukocytes of the Alcoholics

Staphylococcus aureus를 이용한 주정중독환자 백혈구의 Phagocytic Plaque 형성력

  • 성희경 (인제대학교 상계백병원 임상병리과) ;
  • 서병배 (인제대학교 부산백병원 임상병리과) ;
  • 김용호 (인제대학교 의생명공학대학 임상병리학과)
  • Published : 2002.09.01

Abstract

This study was conducted to develop a method for direct determination of phagocytic activities in human circulatic systems and to measure the phagocytic activities in human leukocytes from the alcoholics, since phagocytic activity was considered to be very important in human immune mechanism at early stage for the health care of the alcoholics. The subjects for this study were 130 among which 95 males and 3 females were diagnosed as alcoholism and 32 was healthy blood donors. A thin layer of heat-killed Staphylococcus aureus Cowan I was placed on a plastic dish and reacted with whole blood to measure the phagocytic plaque formation by human leukocytes. In order to determine the health conditions of the subjects, some clinical laboratory tests, such as white blood cell counts, hemoglobin contents (Hgb), mean corpuscular volume of red blood cells(MCV), serum electrophoresis, B and T-lymphocytes, T-lymphocyte subtypes and phytohemagglutination test were also implemented. Compared to the non-alcoholism, new and old alcoholic inpatients showed statistically significant differences on levels of Hgb and MCV (p<0.05), but showed that T and B-lymphocyte numbers decreased and Helper T cell/Suppressor T cell ratio ($1.6{\pm}0.8$%) increased. Compared to non-alcoholism, phagocytic plaque activities of leukocytes from alcoholic patients decreased significantly and an unusual pattern in phagocytic plaque was observed, showing a strange body and chain shaped phagocytosis. Based upon these results, it is concluded that a phagocytic-plaques of Staphylococcus aureus Cowan I by leukocytes was very simple and useful method for the early immunological determination of phagocytic activities in alcoholic patients without requiring any special equipments.

주정중독환자에서 초기 비특이적 면역력의 주된 반응인 백혈구 탐식작용을 이용하여 이들 환자들의 건강관리를 용이하게 할 수 있는지를 알아 보기위하여 백혈구 탐식 작용력을 측정하고자 실시하였고, 연구대상은 주정중독 의존형으로 진단된 남자 95명, 여자 3명과 대조군으로서는 건강한 헌혈자 32명을 대상으로 실시하였다. 또한 주정중독환자의 건강상태를 확인하기 위하여 백혈구수, 혈색소, 적혈구 평균용적, 혈청 단백질 전기영동, 림프구 아형 분리 및 림파구 유약화 시험도 실시하였따. 분석결과에서 혈색소와 적혈구 평균용적은 대조군에 비하여 신, 장기 입원 환자군에서 유의할만한 차이를 보였으며(P<0.05), 총 T와 B 림프구는 감소하였고, $T_{Helper}/T_{suppressor}$ 비율은 $1.6{\pm}0.8%$로 증가하였다. 특히 신, 장기입원 주정중독자의 Staphylococcus aureus Cowan I을 사용한 백혈구의 phagocytic plaque 형성력에서도 대조군에 비하여 현저하게 저하되었고, 이제까지 보고된 바 없는 특이한 strange body, 사슬상 탐식현상을 관찰 할 수 있었다. 따라서 Staphylococcus aureus Cowan I을 사용한 백혈구의 phagocytic plaque 형성 시험은 특별한 기구가 없이 간단하게 이용 가능하여 주정중독환자의 초기 면역력 측정에 매우 유용한 방법임을 알 수 있었다.

Keywords

References

  1. 임상병리학회지 v.12 no.3 알코올 중독 환자에서의 평균 적혈구 용적에 관한 고찰 권석운;박성호;나덕렬
  2. 신경정신의학 v.27 no.6 알코올의 이화학적소견에 대한 약물남용의 영향 권정화;Michael Chang
  3. 신경정신의학 v.30 no.3 한국형 알코올 중독 선별검사 제작을 위한 예비연구(Ⅲ) 김경빈;한광수;이정국
  4. 인간생명과학 소화기 박재갑
  5. 혈청 단백 분획상 서덕규
  6. 최신의학 v.28 no.8 Ethanol이 생체 blood glucose 및 epinephrine 농도에 미치는 영향 이한기
  7. 신경정신의학 v.33 no.1 주정 중독 환자에서 Fluoxetine이 혈중 알콜 농도와 음주효과에 미치는 영향 정봉주;박제민;김명정
  8. HLA Technical workshop The orignal and leader in microtesting Athens, G.
  9. Br. J. Haematol. v.43 no.4 Mean cell volume in a working population: the effect of age, smoking, alcohol and oral contraception Chalmers, D.M;A.J. Levi;I. Chanarin https://doi.org/10.1111/j.1365-2141.1979.tb03796.x
  10. Lancet v.6 Mean cell volume and gamma-glutamyl-transpeptides as markers of drinking in working men Chick, J.;N. Kreitman;M. Plant
  11. Blood. v.52 no.3 Effect of acute alcohol intoxication on granulocyte mobilization and kinetics Gluckman S.J.;R.R. MacGregor
  12. Practical Immunology Hay, F.C.
  13. J. Leukoc. Biol. v.39 no.5 Effect of ethanol administration on parameters of immunocompetency in rats Jerrells, T.R.;C.A. Marietta;M.J. Eckardt;E. Majchrowicz;F.F. Weight https://doi.org/10.1002/jlb.39.5.499
  14. Cell. Imminol. v.102 no.1 A novel mechanism of immuno suppression mediated by ethanol Kaplan, D.R. https://doi.org/10.1016/0008-8749(86)90320-5
  15. Blood. v.34 no.6 Deteminants of erythocyte size in chronic liver disease Kilbridge, T.M.;P. Heller
  16. Jikeikai med. J. v.15 The papplinance of biological characteistics of Staphylococcus aureus organism for some experimental systems Masuda, S.
  17. Proc. Soc. .Exp. Biol. Med. v.190 no.1 Phytohemagglulutinin skin test responses to evaluate in vivo cellular immune function in rats Mendenhall, C.L.;C.J. Grossman;G.A. Roselle https://doi.org/10.3181/00379727-190-42838
  18. Effects of various alcohols applied in vitro on human lymphocyte and AIDS Parbhhala, R.H.;R.R. Watson
  19. Eng. J. med. v.282 no.3 Effect of alcohol and various disease on leukocyte mobilization, phagocytosis and intracellular bacterial killing Robert, G.;B.R. Ayton;Petere https://doi.org/10.1056/NEJM197001152820303
  20. Alcohol. Clin. Exp. Res. v.10 no.6 Conventional and coming laboratory markers of alcoholism and heavy drinking Salaspuro, M. https://doi.org/10.1111/j.1530-0277.1986.tb05174.x
  21. Jikeikai med.J. v.135 A unusual pattern in phagocytic activity of leukocyte from a patient with Behcet's disease in streptococcal monolayer Seki, K.;M. Murai;J. Sakurada
  22. Microbiol. Immunol. v.33 A simple method for observation of phagocytosis on bacterial thin-layer Seki, K.;M. Murai;J. Sakurada;A. Shirahige;N. Kobayashi;S.M. Hwang;S. Masuda https://doi.org/10.1111/j.1348-0421.1989.tb01500.x
  23. Am. J. Med. Sci. v.267 Red blood cell mean corpuscular volume: a potential indicator of alcohol usage in a working population Unger, K.W.;D. Johnson Jr. https://doi.org/10.1097/00000441-197405000-00004
  24. Alcohol. Clin. Exp. Res. v.9 Cellular immune functions, endorphins, and alcohol consumption in males Watson, R.R.;J.C. Jackson;B. Hartmann;R. Sampliner;D. Mobley;C. Eskelson https://doi.org/10.1111/j.1530-0277.1985.tb05745.x