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Biological Assay and Cytopathological Characteristics of Grapevine leafroll-associated 3 virus (GLRaV-3) and Grapevine fanleaf virus (GFLV)

  • Kim, Hyun-Ran (Horticultural Environment Division, National Horticultural Research Institute, Rural Development Administration) ;
  • Park, Yong-Mun (Horticultural Environment Division, National Horticultural Research Institute, Rural Development Administration) ;
  • Chung, Bong-Nam (Horticultural Environment Division, National Horticultural Research Institute, Rural Development Administration) ;
  • Park, Gug-Seoun (Horticultural Environment Division, National Horticultural Research Institute, Rural Development Administration) ;
  • Kim, Jeong-Soo (Horticultural Environment Division, National Horticultural Research Institute, Rural Development Administration)
  • Published : 2002.01.01

Abstract

Grapevine leafroll-associated 3 virus (GLRaV-3) and Grapevine fanleaf virus (GFLV) are important viral diseases of grapevine in the world. In this study, the most reliable woody indicator plants were selected for virus indexing. Two grapevines, LN33 (Couderc 1613x vitis berlandieri) and Vitis riparia Gloire, were selected for CLRaV-3 and CFLV graft indexing, respectively. The specific characteristics of Closterovirus isolated from grapevines cultivated in Korea were identified. filamentous virus-like particles only existed in the phloem parenchyma cell. In particular, the vesiculation of mitochondria was observed. This mitochondrial vesicu-lation was considered to be one of the most reliable cytopathic features of Closterovirus. During observation of GFLV-infected Chenopodium quinoa sections, virus-like particles arranged consistently were found forming several layers in cytoplasm. Moreover, virus-like particles in tubules were observed and were associated with plasmodesmata in cytoplasm. This is the first report on cytopathological characteristics of Closterovirus and Nepovirus identified from grapevines in Korea.

Keywords

References

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