Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Adenovirus-Mediated Antisense Telomerase with Cisplatin Increased the Susceptibility of Cisplatin Resistant Ovarian Cancer Cell Line

  • Kim, Dae-Shick (Department of Pathology, Sung Kyun Kwan University, School of Medicine) ;
  • Song, Joon-Seok (Institute of Biotechnology, Anam Hospital Korea University) ;
  • Lee, Kyu-Wan (Department of Obsterics and Gynecology, Anam Hospital, Korea University) ;
  • Kim, Mee-Hye (Sewha Pediatric Clinic) ;
  • Kim, Kyung-Tai (Department of Obstetrics and Gynecology, Hanyang University) ;
  • Kim, Hysook (Department of Pathology, Sung Kyun Kwan University, School of Medicine) ;
  • Kim, Young-Tae (Department of Obsterics and Gynecology, Anam Hospital, Korea University)
  • Published : 2002.10.01
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Abstract

Telomerase adds telomeric repeats to chromosomal ends and is known to play an important role in carcinogenesis through cellular immortalization. Since telomerase is an essential pathogenomic factor in malignant tumors, inhibiting telomerase activity is thought to be possible to make telomerase positive tumors more sensitive to cisplatin treatment, which is effective in ovarian cancers, but clinical success Is limited by chemo-resistance. In the present study, cisplatin-sensitive ovarian cancer cell line A2780 and cisplatin-resistant A2780/cp70 cell line were infected with antisense telomerase adenovirus Ad-OA. It was found that the Ad-OA suppressed ovarian cancer cell growth and this effect was mainly due to the induction of caspase-dependent apoptosis. Next, we infected the cisplatin resistant ovarian cancer cell line A2780/ cp70 with Ad-OA and cisplatin concurrently. Interestingly, cisplatin treatment with Ad-Oh was more effective to cisplatin-induced cell death in A2780/cp70 cells compared to cisplatin or the vector group only. These data suggest that cisplatin treatment with Ad-OA may be a new chemo-sensitizer for cisplatin resistant ovarian cancer.

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