Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Dietary Supplementation of Sea Tangle Extracts on the Excretion of Neutral Steroids and Bile Acid in Diabetic Rats

다시마 추출물의 급여가 당뇨쥐의 중성스테로이드와 담즙산 배설에 미치는 영향

  • 장민아 (영남대학교 식품영양학과) ;
  • 이경순 (영남대학교 식품영양학과) ;
  • 서정숙 (영남대학교 식품영양학과) ;
  • 최영선 (대구대학교 식품영양학과)
  • Published : 2002.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

The present study was conducted to investigate the effect of dietary supplementation of sea tangle extracts on the excretion of neutral steroids and bile acid in diabetic rats. Sprague-Dawley rats were fed on AIN-76 based experimental diets containing methanol extracts (2%, w/w) or water extracts (4%, w/w) of sea tangle for 4 weeks. And then they were induced to be diabetic by receiving streptozotocin (45 mg/kg BW) intramuscularly. The dried fecal weight was increased significantly in water extracts group compared with control group. The fecal content of cholesterol was higher in extracts groups of sea tangle than in control group. But there was no significant difference in fecal excretion of cholesterol between methanol and water extracts groups. The fecal excretion of coprostanol was increased significantly in water extracts group compared with the control group. The fecal excretion of bile acid was increased significantly in sea tangle extracts groups compared to the control group. These data suggest that dietary supplementation of sea tangle extracts might reduce the incidence of atherosclerosis through increasing the excretion of neutral steroids.

본 연구는 메탄올과 물에서 각각 추출한 다시마 추출물을 급여한 흰쥐에게 당뇨를 유도하고 다시마 추출물의 섭취가 당뇨병의 혈관계 합병증의 유발과 관련되는 체내 지질대사의 변화에 미치는 영향을 조사하고자 하였다. 흰쥐에게 다시마의 메탄올추출물(2%, w/w)과 물추출물(4%, w/w)을 식이에 첨가하여 4주간 급여하고, streptozotocin(STZ, 45 mg/kg BW)으로 당뇨를 유도한 후 혈당, 혈중 콜레스테롤과 중성지질, 변의 수분 함량과 중량, 변의 스테로이드 및 담즙산 함량을 측정하였다. 당뇨 유발로 인해 흰쥐의 체중 감소가 현저하였으며 식 이에 따른 유의적인 차이는 나타나지 않았지만 메탄올추출물 급여군보다 물추출물 급여군에서 당뇨에 의한 체중감소가 적은 것으로 나타났다. 식이에 따른 식이효율의 차이는 나타나지 않았으나 식이 섭취량은 물추출물 급여군이 유의적으로 낮은 경향이었다. 혈당 변화는 당뇨를 유도하고 다시마추출물을 급여하지 않은 대조군에 비해 다시마추출물 급여군에서 유의적으로 감소하였으나 추출물의 종류에 따른 차이는 나타내지 않았다. 중성지방 함량은 대조군에 비해 다시마추출물을 급여한 군에서 중성 지질 함량이 유의적으로 감소하는 경향을 나타내었으나 추출물간의 차이는 관찰되지 않았다. 인지질 함량은 다시마추출물 급여군에 비해 대조군에서 약간 높았으나 메탄올추출물과 물추출물간의 차이는 관찰되지 않았다. 혈장에서의 총 콜레스테롤 함량은 실험군간의 차이를 보이지 않았다 HDL-콜레스테롤 함량은 실험군간의 유의적인 차이는 없으나 대조군과 메탄올추출물 급여군에 비해 물추출물 급여군에서 그 함량이 높았다 분변의 건조중량은 다시마의 물추출물 급여군에서 유의적으로 증가하였다. 분변 중의 수분 함량은 유의적이지는 않지만 대조군이나 메탄올추출물 급여군보다 물추출물 급여군에서 증가하는 경향이었다. 변으로 배설되는 콜레스테롤 함량은 메탄올과 물에서 각각 추출한 다시마 추출물 급여군이 대조군에 비해 유의적으로 높았으며, 특히 물추출물 급여군에서 가장 많이 배설되었다. 하루에 변으로 배설되는 coprostanol함량은 다시마의 물추출물 급여군에서 유의적으로 증가되었다 변으로 배설되는 담즙산의 함량은 메탄올과 물추출물 급여군에서 대조군에 비해 유의적으로 높았다 이상과 같은 결과를 통해서 볼 때 다시마추출물 섭취는 콜레스테롤과 같은 중성스테로이드의 배설을 촉진시킴으로써 당뇨로 인해 발생하기 쉬운 합병증인 동맥 경화증의 예방적 효과에 기여할 수 있으리라 사료된다.

Keywords

References

  1. Baynes JW. 1991. Role of oxidative stress in development of complications in diabetes. Diabetes 40: 405-412.
  2. Strachan WJ, Deary IJ. 1997. Is type II diabetes associated with an increased risk of cognitive dysfunction? Diabetes Care 20: 438-445. https://doi.org/10.2337/diacare.20.3.438
  3. Han XL, Abendschein DR, Kelley JG, Gross RW. 2000. Diabetes-induced changes in specific lipid molecular species in rat myocardium. Biochem J 352: 79-89. https://doi.org/10.1042/0264-6021:3520079
  4. Ginsgerg H. 1991. Lipoprotein physiology in non-diabetic and diabetic states: relationship to atherogenesis. Diabetes Care 14: 839-855.
  5. Best JD, O'Neal DN. 2000. Diabetic dyslipidaemia-Current treatment recommendations. Drugs 59: 1101-1111. https://doi.org/10.2165/00003495-200059050-00006
  6. Gibbones GY. 1996. Hyperlipidemia of diabetes. Clin Sci 71: 477-486.
  7. Jonnalagadda SS, Thye FW, Robertson JL. 1993. Plasma total and lipoprotein cholesterol, liver cholesterol and fecal cholesterol excretion in hamsters fed fiber diets. J Nutr 123: 1377-1382.
  8. Song YS, Yang JL, Suh MJ. 1996. Effects of sodium alginate and cellulose on gastrointestinal physiology in rats. J Korean Soc Food Sci Nutr 25: 551-559.
  9. Torsdottir I, Alpsten M, Holm G, Sandberg AS, Tolli J. 1991. A small dose of soluble alginate-fiber affects postprandial glycemia and gastric emptying in humans with diabetes. J Nutr 121: 795-799.
  10. Wursh P, Pi-Sunyer FX. 1997. The role of viscous soluble fiber in the metabolic control of diabetes. Diabetes Care 20: 1774-1780. https://doi.org/10.2337/diacare.20.11.1774
  11. AIN standard for nutrition studies report. 1997. J Nutr 107: 1340-1348.
  12. Crowell MJ, Macdonald IA. 1980. Enzymic determination of 3$\alpha$-, 7$\alpha$-, and 12$\alpha$-hydroxyl groups of fecal bile salts. Clin Chem 26: 1298-1300.
  13. Bennett WG, Cerda JJ. 1996. Benefits of dietary fiber. Postgraduated Medicine 99: 153-175.
  14. Broderick GA, Walgenbach RP, Sterrenburg E. 2000. Performance of lactating dairy cows fed alfalfa or red clover silage as the sole forage. J Dairy Sci Assoc 83: 1543-1551. https://doi.org/10.3168/jds.S0022-0302(00)75026-0
  15. Boden G. 1997. Role of fatty acrd in the pathogenesis of insulin resistance and NIDDM. Diabetes 46: 3-10.
  16. Reaven KM. 1987. Abnormal lipoprotein metabolism in noninsulin dependent diabetes mellitus. Am J Med 83: 31-40.
  17. Park EY, Lee SS. 1996. Effect of dietary fiber on the serum lipid level and bowel function in aged rats. Kor J Nutr 29: 934-942.
  18. Park HS, Kim KH, Yoon HS. 1997. Comparison on the mechanism of hypocholesterolemic effect of dietary fats and fibers in DMH-treated rats. Kor J Lipidology 7: 141-153.
  19. Wang C, Yang G. 1997. Comparison of effects of two kinds of soluble algae polysaccharide on blood lipid, liver lipid, platelet aggregation and growth in rats. NCBI 31: 342-345.
  20. Son HS, Kim HS, Ju JS. 1992. Effect of seaweed intake on the absorption of sodium, calcium, potassium and hypolipidemic mechanism in healthy male subjects. J Korean Soc Food Nutr 21: 471-477.
  21. Lee HS, Choi MS, Lee YK, Park SH, Kim YJ. 1996. A study on the development of high-fiber supplements for the diabetic patients (I) - Effect of seaweed supplementation on the gastrointestinal function and diabetic symptom control in streptozotocin-induced diabetic rats - Korean J Nutr 29: 286-295.
  22. Innami S, Nakamura K, Tabata K, Wada M, Takita T. 1995. Water-soluble viscous substance of Jew's Mellow leaves lowers serum and liver cholesterol concentrations and increases fecal steroid excretion in rats fed a high cholesterol diet. J Nutr Sci Vitaminol 41: 464-475.
  23. Lee KS. 1998. Effect of sea tangle and hypoglycemic agent on nutrient status and intestinal ultrastructure in diabetic rat. PhD Dissertation. Yeungnam University.
  24. Haffmer SM. 1998. Management of dyslipidemia in adults with diabetes. Diabetes Care 21: 160-178. https://doi.org/10.2337/diacare.21.1.160
  25. Palmer AM, Thomas CR, Gopaul N, Dhir S, Anggard EE, Poston L, Tribe RM. 1998. Dietary antioxidant supplementation reduces lipid peroxidation but impairs vascular function in small mesenteric arteries of the streptozotocin-diabetic rat. Diabetologia 41: 148-156. https://doi.org/10.1007/s001250050883
  26. Freitas JP, Filipe PM, Rodrigo FG. 1997. Lipid peroxidation in type 2 normolipidemic diabetic patients. Diabetes Res Clin Prac 36: 71-71. https://doi.org/10.1016/S0168-8227(97)00032-6
  27. Moundras C, Behr SR, Remesy C, Demigne C. 1997. Fecal Losses of sterols and bile acids induced by feeding rats guar gum are due to greater pool size and liver bile acid secretion. J Nutr 127: 1068-1076.
  28. Sandberg AS, Andersson H, Bosoeus I, Carlsson NG, Hasselblad K. 1994. Alginate, small bowl sterol excretion, and absorption of nutrients in ileostomy subjects. Am J Nutr 60:751-756.
  29. Nyman M, Schweizer TF, Tyren S, Reimann S, Asp NG. 1990. Fermentation of vegetable fiber in the intestinal tract of rats and effects on fecal bulking and bile acid excretion. J Nutr 120: 459-466.
  30. Jenkins DJA, Spadafora PJ, Jenkins AL, Rainey-Macdonald CG. 1992. Fiber in the treatment of hyperlipidemia. In CRC Handbook of dietary fiber in human nutrition. 2nd ed. Spiller GA, ed. CRC Press, USA. p 419-438.
  31. Ikegami S, Tsuchihashi F, Harada H, Tsuchihashi N, Nishide E, Innami S. 1990. Effect of viscous indigestible polysaccharides on pancreatic-biliary secretion and digestive organs in rats. J Nutr 120: 353-360.

Cited by

  1. Effects of Dietary Supplementation of Alga Mixtures (Hizikia fusiformis and Ecklonia cava) on Innate Immunity and Disease Resistance Against Edwardsiella tarda in Olive Flounder (Paralichthys olivaceus) vol.42, pp.6, 2009, https://doi.org/10.5657/kfas.2009.42.6.614
  2. Effects of Sea Tangle-added Patty on Postprandial Serum Lipid Profiles and Glucose in Borderline Hypercholesterolemic Adults vol.43, pp.4, 2014, https://doi.org/10.3746/jkfn.2014.43.4.522
  3. Contents of Water Extract for Laminaria japonica and its Antioxidant Activity vol.26, pp.2, 2011, https://doi.org/10.7841/ksbbj.2011.26.2.112
  4. Effect of Seatangle Oligosaccharide Drink on Serum and Hepatic Lipids in Rats Fed a Hyperlipidemic Diet vol.32, pp.8, 2003, https://doi.org/10.3746/jkfn.2003.32.8.1364
  5. Sea Tangle Supplementation Lowers Blood Glucose and Supports Antioxidant Systems in Streptozotocin-Induced Diabetic Rats vol.7, pp.2, 2004, https://doi.org/10.1089/1096620041223996
  6. 다시마추출물이 제2형 당뇨병 환자의 혈당, 지질 및 항산화 체계에 미치는 영향 vol.36, pp.11, 2007, https://doi.org/10.3746/jkfn.2007.36.11.1391