Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지

Biological Activities of Ethanol Extracts and Fractions of Black Olympia Grape(Vitis Labruscana L.)

거봉 포도종의 에탄올 추출물 및 분획물에 대한 생리활성 효능

  • 박성진 (강원대학교 바이오산업공학부) ;
  • 박부길 (강원대학교 바이오산업공학부) ;
  • 이현용 (강원대학교 바이오산업공학부) ;
  • 오덕환 (강원대학교 바이오산업공학부)
  • Published : 2002.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to determine biological activities, such as lipid peroxidation inhibition and cytotoxic effect of ethanol extracts of Black Olympia grape seeds and skins, and of organic solvent fractionated ethanol extracts obtained from grape seeds and skins at different temperatures. Among different extraction temperatures, the ethanol extract of grape seed obtained at 30$\^{C}$ had the strongest lipid oxidation inhibition of 60.1%, while the strongest lipid oxidation inhibitory effect of 71.2% was observed in the presence of 20 $\mu\textrm{g}$/㎖ ethylacetate fraction obtained from ethanol extract of grape seeds at 30$\^{C}$. The ethanol extract of grape seeds showed more strong lipid oxidation inhibition than that of skin extracts. Similar results were observed in cytotoxic effects. The ethanol extract of grape seeds at 30$\^{C}$ exhibited more strong cytotoxicity than that of skin extracts on MCF-7, Hep3B, and A549 cell lines. Among organic solvent fractions extracted from the ethanol extracts of gape seeds and skins, the hexane fraction showed the strongest cytotoxic inhibition of 75.15% and 62.50% on MCF-7 and Hep3B cell in the presence of 1.0 $\mu\textrm{g}$/㎖ respectively. On the other hand, the water fraction showed the strongest cytotoxic inhibition of 65.41% on A549 cell in the presence of 1.0 $\mu\textrm{g}$/㎖. Overall, the ethanol extracts and their fractions of Black Olympia grape seeds showed strong lipid oxidation inhibition and cytotoxicity than those of grape skins.

본 연구는 포도 거봉종의 종자와 과피 추출물의 지질과산화 억제 효과와 암세포들에 대한 세포 독성을 살펴보았다. 추출 온도를 달리하여 추출한 종자와 과피의 에탄올 추출물과 추출물에 대한 분획물들을 이용하여 지질과산화물의 억제효과를 측정한 결과, 종자 및 과피 에탄올 추출물 모두 온도변화에 따른 영향은 없는 것으로 나타났으며, 추출물의 농도가 증가할수록 지질과산화물의 감소효과가 증가하였다. 종자 추출물의 경우, 3$0^{\circ}C$에서 추출한 에탄올추출물이 20 $\mu\textrm{g}$/$m\ell$ 농도에서 60.1%, 분획물 중에서는 ethyl acetate 분획물이 71.2%의 지질과산화를 억제하였다. 과피 에탄올 추출물의 경우, 5$0^{\circ}C$에서 추출한 추출물이 20 $\mu\textrm{g}$/$m\ell$ 농도에서 48.1%, 분획물 중에서는 hexane 분획물이 44.4%의 지질과산화를 각각 억제하였다. 암세포 생육 억제 효과에서는 모든 암세포(MCF-7, Hep3B 및 A549)에서 포도 종자 및 과피 에탄올 추출물 및 분획물들이 1.00 $\mu\textrm{g}$/ML 농도에서 대부분 50% 이상의 생육 억제율을 보였으며, 암세포에 대한 생육 억제와 정상 세포에 대한 selectivity에서는 종자 에탄올 추출물과 과피 에탄을 추출물간의 유의적인 차이는 나타나지 않았다. 본 연구 결과, 거봉포도종자 및 과피의 에탄올 추출물들과 각 분획물은 지질과산화 억제 효과와 암세포들에 세포 독성이 캠벨종의 포도종자 및 과피에 비하여 낮은 활성을 나타내었다.

Keywords

References

  1. Blavo, L. (1998) Polyphenols: Chemistry, dietary sources, metabolism and nutritional significance. Nutr. Rev. 56, 317-333 https://doi.org/10.1111/j.1753-4887.1998.tb01670.x
  2. Lee, J.H. and Lee, S.R. (1994) Some physiological activity of phenolic substances in plant foods. Korean J. Food Sci. Technol. 26, 317-323
  3. Manzocco, L., Anese, M., and Nicoli, M.C. (1998) Antioxidant properties of tea extracts as affected by processing. Lebensm.-Wiss. U. Technol. 31, 694-698 https://doi.org/10.1006/fstl.1998.0491
  4. Shirataki, Y., Kawase, M., Saito, S., Kurihara, T., Tanaka, W., Satoh, K., Sakagami, H., and Motohashi, N. (2000) Selective cytotoxic activity of grape peel and seed extracts against oral tumor cell lines. Anticancer Res. 20, 423-426
  5. Bagchi, D., Garg, A., Krohn, R.L., Bagchi, M., Tran, M.X., ad n Stohs, S.J. (1997) Oxygen free radical scavenging abilities of vitamins C and E, and a grape seed proanthocyanidin extract in vitro. Res. Commun. Mol. Pathol. Pharmacol. 95, 179-189
  6. Zhao, J., Wang, J., Chen, Y., and Agarwal, R. (1999) Anti-tumor-promoting activity of a polyphenolic fraction isolated from grape seeds in the mouse skin two-stage initiation-promotion protocol and identification of procyanidin B5-3'-gallate as the most effective antioxidant constituent. Carcinogenesis 20, 1737-1745 https://doi.org/10.1093/carcin/20.9.1737
  7. Piver, B., Berthou, F., Dreano, Y., and Lucas, D. (2001) Inhibition of CYP3A, CYP1A and CYP2E1 activities by resveratrol and other non volatile red wine components. Toxicology Letters 125, 83-91 https://doi.org/10.1016/S0378-4274(01)00418-0
  8. Palma, M., and Taylor, L.T. (1999) Extraction of polyphenolic compounds from grape seeds with near critical carbon dioxide. J. Chromatogr. A. 849, 117-124 https://doi.org/10.1016/S0021-9673(99)00569-5
  9. Cho, S.Y., Han, Y.B. and Shin, K.H. (2001) Screening for antioxidant activity of edible plants. J. Korean Soc. Food Sci. Nutr. 30, 133-137
  10. Kim S.K., Kim Y.G., Lee M.K., Han J.S., Lee J.H., and Lee H.Y. (2000) Comparison of biological activity according to extracting solvents of four Acanthopanax root Bark. J. Medicinal Crop Sci. 8, 21-28
  11. Carnesecchi, S., Schneider, Y., Lazarus, A.S., Coehlo, D., Gosse, F., and Raul, F. (2002) Flavanols and procyanidins of cocoa and chocolate inhibit growth and polyamine biosynthesis of human colonic cancer cells. Cancer Letters 175, 147-155 https://doi.org/10.1016/S0304-3835(01)00731-5
  12. Singletary, K.W., and Meline, B. (2001) Effect of grape seed proanthocyanidins on colon aberrant crypts and breast tumors in a rat dual-organ tumor model. Nutr. Cancer. 39, 252-258 https://doi.org/10.1207/S15327914nc392_15
  13. Bagchi, D., Garg, A., Krohn, R.L., Bagchi, M., Bagchi, D.J., Balmoori, J., and Stohs, S.J. (1998) Protective effects of grape seed proanthocyanidins and selected antioxidants against TPA-induced hepatic and brain lipid peroxidation and DNA fragmentation, and peritoneal macrophage activation in mice. Gen. Pharmacol. 30, 771-776 https://doi.org/10.1016/S0306-3623(97)00332-7
  14. Ye, X., Krohn, R.L., Liu, W., Joshi, S.S., Kuszynski, C.A., McGinn, T.R., Bagchi, M., Preuss, H.G., Stohs, S.J., and Bagchi, D. (1999) The cytotoxic effects of a novel IH636 grape seed proanthocyanidin extract on cultured human cancer cells. Mol. Cell Biochem. 196, 99-108 https://doi.org/10.1023/A:1006926414683