Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of YK-209 Mulberry Leaves on Antioxidative Defense System of Liver in Streptozotocin-Induced Diabetic Rats

YK-209 뽕잎이 Streptozotocin 유발 당뇨쥐 간조직의 항산화계에 미치는 영향

  • 유수경 (대구카톨릭대학교 식품영양학과) ;
  • 이순재 (대구카톨릭대학교 식품영양학과)
  • Published : 2002.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study was investigated the effects of YK-209 mulberry leaves on antioxidative defense system of liver in diabetic rats induced with streptozotocin (STZ). Male Sprague-Dawley rats weighing 100$\pm$10 g were randomly assigned to one normal and four STZ-induced diabetic groups; YK-209 mulberry leaves free diet (DM group),0.1% YK-209 mulberry leaves diet (DM-0.1Y group),0.2% YK-209 mulberry leaves diet (DM-0.2Y group) and 0.4% YK-209 mulberry leaves diet (DM-0.4Y group). Diabetes was induced by intravenous Injection of 55 mg/kg body weight of STZ in sodium citrate buffer (pH 4.3) via tail vein after 4 weeks feeding of experimental diets. Rats were sacrificed at the 9th day of diabetic states. Liver weight in all four diabetic groups were higher than normal group, but YK-209 mulberry supplementation groups were lower than DM group. Hepatic superoxide dismutase (SOD) activity was significantly decreased in all diabetic groups, compared with normal group. Hepatic glutathione peroxidase (GSHpx) activity was 7.3% decreased in DM group, compared with normal group, but those of DM-0.1Y and DM-0.2Y groups were maintained the normal level. The hepatic thiobarbituric acid reactive substances was markedly increased by 144% in DM group, compared with normal group, but those of DM-0. 1Y, DM-0.2Y groups were maintained the normal level. The contents of lipofuscin in liver were increased by 100% in DM group compared with normal group, but those of DM-0. 1Y, DM-0.2Y and DM-0.4Y groups were decreased to 42% 43% and 44%, respectively, compared with DM group. The hepatic superoxide radical (0$^2$-) contents in DM group were increased to 81%, compared with normal group, but those of DM-0.1Y and DM-0.4Y groups were similar to those of normal group. The present result indicate that YK-209 mulberry leaves regarded to suppress lipid peroxidation as an free radical scavenger system by the inhibition of oxidative stress.

YK 209 뽕잎이 STZ 유발 당뇨쥐 간조직의 항산화계에 미치는 영향을 관찰코져 하였다. Sprague Dawley 흰쥐 수컷을 정상군(normal group)과 STZ 유발 당뇨군으로 나누고 당뇨군은 다시 식이내 YK-209뽕잎 함량에 따라 각각 YK-209뽕잎을 공급하지 않은 당뇨대조군(DM group),0.1% 뽕잎 공급군(DM-0.IY group), 0.2% 뽕잎 공급군(DM-0.2Y group) 및 0.4% 뽕잎 공급군 (DM-0.4Y group)으로 나누어 자유섭식 시켰다. 3주후 STZ으로 당뇨를 유발한 후 9일째에 희생하였다. 단위체중당 간무게는 정상군에 비해 당뇨군 모두 유의적으로 증가되었으나 뽕잎 비공급 당뇨군(DM group)에 비해 뽕잎공급군들은 감소되었다. 간조직중의 SOD 활성은 정상군에 비해 당뇨유발군에서 유의적으로 감소하였으며 뽕잎공급으로 인한 차이는 없었다. GSHpx 활성은 정상군에 비해 DM군은 7.3%저하되었으나, DM-0.1Y, DM-0.2Y군은 정상군 수준이었다. 간조직 중의 지질과산화가는 정상군에 비해 DM군은 144%증가되었으나 DM-0.1Y, DM-0.2Y군은 정상군 수준이었다. 리포푸신 함량은 정상군에 비해 DM군에서 100%증가되었으나 YK-209 뽕잎 공급군은 DM군에 비해 유의적으로 감소되었다. 간조직 중의 superoxide radical(O$_2$ ) 함량은 정상군에 비해 DM군에서는 81% 증가되었으나 DM-0.1Y군과 DM-0.4Y군은 정상군과 유의적인 차이가 없었다. 이상의 결과에서 YK-209 뽕잎은 항산화계를 강화시켜 조직의 산화적 손상을 감소시키므로서 산화적 스트레스 억제 효과가 있었다.

Keywords

References

  1. Baynes JW. 1991. Role of oxidative stress in development of complications in diabetes. Diabetes 40: 405-412. https://doi.org/10.2337/diabetes.40.4.405
  2. West IC. 2000. Radicals and oxidative stress in diabetes. Diabet Med 17: 171-180. https://doi.org/10.1046/j.1464-5491.2000.00259.x
  3. Obrosova IG, Fathallah L, Green DA. 2000. Early changes in lipid peroxidation and antioxdative defense in diabetic rat retina: effect of DL-alpha-lipoic acid. Eur J Pharmacol 398: 139-146. https://doi.org/10.1016/S0014-2999(00)00286-7
  4. Jain SK, McVie R, Jaramillo JJ, Palme M, Smith T, Meachum ZD, Little RL. 1996. The effect of modest vitamin E supplementation on lipid peroxidation products and other cardiovascular risk factors in diabetic patients. Lipids 31: S87-S90. https://doi.org/10.1007/BF02637057
  5. Maytin M, Leopold J, Leopold J, Lascalzo J. 1999. Oxidant stress in the vasculature. Curr Atheroscler Rep 1: 156-164. https://doi.org/10.1007/s11883-999-0012-z
  6. Sato Y, Hotta N, Sakamoto N, Matsuoka S, Ohishi N, Yagi K. 1979. Lipid peroxide level in plasma of diabetic patients. Biochem Med 21: 104-107. https://doi.org/10.1016/0006-2944(79)90061-9
  7. Kwag OG, Yang JA, Rhee SJ. 1999. Effects of vitamin E on the antioxidative defense system of kidney in streptozotocin -induced diabetic rats. J Korean Soc Food Sci Nutr 28: 654-662.
  8. Chai YM, Park MR, Rhee SJ. 1998. Effects of green tea catechins on the antioxidative defense system and lipofuscin levels heart in streptozotocin-induced diabetic rats. Kor J Gerontol 8: 96-103.
  9. Lalla E, Lamster IB, Drury S, Fu C, Schmidt AM. 2000. Hyperglycemia, glycoxidation and receptor for advanced glycation endproducts: potential mechanisms underlying diabetic complications, including diabetes-associated periodontitis. Periodontol 23: 50-62. https://doi.org/10.1034/j.1600-0757.2000.2230104.x
  10. Naitoh K. 1968. Studies on the micro constituent in mulberry leaves part 2. Isolation of rutin and quercetin from mulberry leaves. Nippon Nogei Kagaku Kaishi 42: 422-425.
  11. Shin KH, Young HS, Lee TW, Choi JS. 1995. Studies on the chemical component and antioxidative effects of solanum lyratum. Kor J Pharmacogn 26: 130-138.
  12. Kim JS, Kang SS, Lee MW, Kim OK. 1995. Isolation offlavonoids from the leaves of Aralia continentails. Kor J Phamacogn 26: 239-243.
  13. Onogi A, Osawa K, Yasuda H, Sakai A, Morita H, Tokawa H. 1993. Flavonol glycosides from the leaves of Morus alba. Shoyakugaku Zasshi 47: 423-425.
  14. Kang SS, Woo WS. 1984. Flavonol glycosides from the leaves of Zizyphus jujuba. Kor J Pharmacogn 15: 170-178.
  15. Kim SY, Lee WC, Kim HB, Kim SK. 1998. Antihyperlipidemic effects of methanol extracts from mulberry leaves in cholesterol induced hyperlipidemia in rats. J Kor Soc Food Sci Nutr 27: 1217-1222.
  16. Kim SY, GA JJ, Lee WC, Ryu KS, Lee KR, Kim YC. 1999. Antioxidative flavonoids from the leaves of Morus alba. Arch Pharm Res 22: 81-85. https://doi.org/10.1007/BF02976442
  17. Marklund S, Marklund G. 1974. Involvement of the superoxide anion radical in the antioxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47: 469-474. https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  18. Lawrence RA, Burk RF. 1976. Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Commun 71: 952-958. https://doi.org/10.1016/0006-291X(76)90747-6
  19. Satoh K. 1978. Serum lipid peroxide in cerebrovascular disorders determined by a new colormetric method. Clin Chim Acta 90: 37-43. https://doi.org/10.1016/0009-8981(78)90081-5
  20. Fletcher BL, Dillard CJ, Tappel AL. 1973. Measurement of fluorescent lipid peroxidation products in biological systems and tissues. Anal Biochem 52: 1-9. https://doi.org/10.1016/0003-2697(73)90327-8
  21. Haper MJK, Catherine J, Norris WE. 1981. Friedrichs and A Moreno Poiy I Caccelerates ovum transport in the rabbit by a prostaglandin-mediated mechanism. J Reprod Fert 63: 81-89. https://doi.org/10.1530/jrf.0.0630081
  22. Azzi A, Montecucco C, Richter C. 1975. The use of acetylated ferricytochrome c for the detection of superoxide radicals produced in biological membranes. Biochem Biophys Res Commun 65: 597-603. https://doi.org/10.1016/S0006-291X(75)80188-4
  23. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the folin phenol reagent. J Biol Chem 193: 265-275.
  24. Griesmacher A, Kindhauser M, Andert SE, Schreiner W, Toma C, Knoebl P, Pietschmann P, Prager R, Schnack C, Schernthaner G. 1995. Enhanced serum levels of thiobarbituricacid-reactive substances in diabetes mellitus. Am J Med 98: 469-475. https://doi.org/10.1016/S0002-9343(99)80347-7
  25. Bus JS, Aust SD, Gibson JE. 1975. Lipid peroxidation: a possible mechanism for paraquat toxicity. Res Commun Chem Pathol Pharmacol 11: 31-38.
  26. Bompart GJ, Prevot DS, Bascands JL. 1990. Rapid automated analysis ofglutathion reductase, peroxidase and S-transferase activity: application to cisplatin-induced toxicity. Clin Biochem 23: 501-504. https://doi.org/10.1016/0009-9120(90)80039-L
  27. Awasthi YC, Beutler E, Srivastava SK. 1975. Purification and properties of human erythrocyte glutathione peroxidase. J Biol Chem 250: 5144-5149.
  28. Chance B, Sies H, Boveris A. 1979. Hydroperoxide metabolism in mammalian organs. Physiol Rev 59: 527-605. https://doi.org/10.1152/physrev.1979.59.3.527
  29. Robak J, Gryglewski RI. 1988. Flavonoids are scavengers of superoxide anions. Biochem Pharmacol 37: 837-841. https://doi.org/10.1016/0006-2952(88)90169-4
  30. Wolff SP, Jiang ZY, Hunt JV. 1991. Protein glycation and oxidative stress in diabetes mellitus and aging. Free Radic Biol Med 10: 339-352. https://doi.org/10.1016/0891-5849(91)90040-A

Cited by

  1. Saengshik, a Formulated Health Food, Decreases Blood Glucose and Increases Survival Rate in Streptozotocin-Induced Diabetic Rats vol.7, pp.2, 2004, https://doi.org/10.1089/1096620041224030
  2. Effect of Red Garlic-Composites on the Fecal Lipid Level and Hepatic Antioxidant Enzyme Activity in Rats Fed a High Fat-Cholesterol Diet vol.42, pp.1, 2013, https://doi.org/10.3746/jkfn.2013.42.1.017
  3. Effects of Dietary Supplementation of Powdered Mulberry Leaves on Egg Production, Egg Quality and Blood Characteristics in Laying Hens vol.39, pp.3, 2012, https://doi.org/10.5536/KJPS.2012.39.3.215
  4. Effects of Dietary Supplementation of Mulberry Leaves Powder on Carcass Characteristics and Meat Color of Broiler Chicken vol.32, pp.6, 2012, https://doi.org/10.5851/kosfa.2012.32.6.789
  5. 뽕잎과 누에가루 혼합환의 Streptozotocin유발 당뇨쥐에서의 혈당강하 효과 vol.33, pp.10, 2004, https://doi.org/10.3746/jkfn.2004.33.10.1611
  6. 고지혈증 환자에서 의학영양치료와 병행하여 섭취한 기능성차(상엽, 구기자, 국화, 대추, 참깨, 나복자)의 혈중 지질 농도 저하 및 항산화 효과 vol.34, pp.1, 2002, https://doi.org/10.3746/jkfn.2005.34.1.042