한국가축번식학회지 (Korean Journal of Animal Reproduction)

한국동물번식학회 (The Korean Society of Animal Reproduction)
권호 표지

항산화제와 Growth Factor 혼합첨가가 돼지 체외수정란의 체외배양에 미치는 영향

Effect of Antioxidants Plus Growth Factors on In Vitro Development of Porcine IVM/IVF Embryos

  • 최영진 (강원대학교 동물자원과학대학) ;
  • 박춘근 (강원대학교 동물자원과학대학) ;
  • 정희태 (강원대학교 동물자원과학대학) ;
  • 김정익 (강원대학교 동물자원과학대학) ;
  • 박동헌 (강원대학교 동물자원과학대학) ;
  • 장현용 (강원대학교 동물자원과학대학) ;
  • 장원경 (축산기술연구소) ;
  • 박진기 (축산기술연구소) ;
  • 양부근 (강원대학교 동물자원과학대학)
  • 발행 : 2002.09.01
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초록

본 연구는 일정량의 항산화제(NAC, ebselen 및 GSH)와 성장인자(EGF, PDGF)의 혼합첨가가 돼지 체외수정란의 체외배양에 미치는 영향을 검토하였다. 1. NCSU 23 배양액에 NAC 1nm과 NAC에 EGF 100ng/$m\ell$비 및 PDGF 5ng/$m\ell$를 각각 혼합 첨가하여 체외배양을 실시한 결과, 상실배기 이상 발육된 체외발육율은 각각 28.1%, 32.3% 및 35.3%로서 NAC와 PDGF 혼합처리구가 대조 구보다 다소 높은 체외발육율을 나타냈으나 통계적 유의성은 없었다(P>0.05). 2. NCSU 23 배양액에 ebselen 10$\mu\textrm{m}$과 ebselen에 EGF 100ng/$m\ell$ 및 PDGF 5ng/$m\ell$를 각각 혼합첨가하여 체외발육율을 조사한 결과, 상실 배기 이상 발육된 수정란의 체외발육율은 각각 17.8%, 36.9% 및 40.3%로 ebselen과 성장인자의 혼합처리구가 대조구보다 통계적 유의하게 높은 체외발육율을 나타냈다(P<0.05). 3. NCSU 23 배양액에 GSH 100$\mu\textrm{m}$과 GSH에 EGF 100ng/$m\ell$ 및 PDGF 5ng/$m\ell$를 각각 혼합 첨가배양하여 상실배기 이상 발육된 수정란의 체외발육율은 각각 24.4%, 30.5% 및 27.7%로 GSH과 EGF 혼합처리구가 여타구보다 다소 높은 체외발육율을 나타냈지만 통계적 유의성은 없었다(P>0.05). 4. 모든 처리구에서 배반포기 수정란의 세포수는 커다란 차이가 인정되지 않았으나(P>0.05), 체외배양액에 ebselen과 성장인자를 혼합첨가하여 체외배양한 처리구에서는 대조구에 비해 통계적으로 유의하게 높은 세포수를 나타냈다.(P<0.05).

Antioxidants(N-acetyl-1-cysteine, ebselen and glutathione) and growth factors(EGF, PDGF) were studied as a mean of increasing the development of porcine embryos produced by in vitro maturation (IVM) and in vitro fertilization(IVF). Porcine embryos developed to the 2~8 cell stage after IVF were cultured fer 6 to 7 days at 38.5$^{\circ}C$ in NCSU 23 medium containing antioxidants plus growth factors. Cell numbers of blastocysts were counted by fluorescence staining method. The developmental rate beyond morula stages in NCSU 23 containing NAC(1nm) or NAC(1nm) plus EGF(100ng/$m\ell$) or PDGF(5ng/$m\ell$) were 28.1, 32.3 and 35.3%, respectively. NAC plus PDGF group was slightly higher than control group(P>0.05). The developmental capacity in NCSU 23 containing ebselen(10 $\mu$M) or ebselen(10 $\mu$M) plus EGF(100ng/$m\ell$) or PDGF(5ng/$m\ell$) were 17.8, 36.9 and 40.3%, respectively Ebselen plus growth factor groups were significantly higher than control group(P<0.05). The developmental capacity in NCSU 23 containing glutathione(100 $\mu$M) or glutathione(100 $\mu$M) plus EGF (100ng/$m\ell$) or PDGF(5ng/$m\ell$) were 24.1, 30.5 and 27.7%, respectively. There were not difference in all experimental groups(P>0.05). In all experimental groups, there was no significantly differences on the cell number of blastocysts, but ebselen plus growth factor groups were significantly higher than control group. These studies indicate that antioxidants plus growth factors can increase the proportion of embryos that developed beyond morulae stage.

키워드

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